Detection of multiple respiratory pathogens during primary respiratory infection: nasal swab versus nasopharyngeal aspirate using real-time polymerase chain reaction

Meerhoff, T J; Houben, Michiel L.; Coenjaerts, Frank E. J.; Kimpen, Jan L. L.; Hofland, Regina W.; Schellevis, François G.; Bont, Louis

doi:10.1007/s10096-009-0865-7

Cited by 83 publications

(81 citation statements)

References 30 publications

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“…These results are similar to other reported non-invasive airway sampling techniques (28,29), but indicated that nasosorption had poorer diagnostic utility than NPA, when using a multiplex PCR assay. However, using a more sensitive single qPCR assay, RSV was detected at 100% sensitivity and 100% specificity, relative to NPA, in line with our previous report (25), thereby validating nasosorption as a tool for respiratory virus detection when using high sensitivity qPCR assays (data not shown).…”

Section: Patients and Ethical Approvalsupporting

confidence: 81%

Reduced Nasal Viral Load and IFN Responses in Infants with Respiratory Syncytial Virus Bronchiolitis and Respiratory Failure

Thwaites

Coates²,

Ito³

et al. 2018

Am J Respir Crit Care Med

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Section: Patients and Ethical Approvalsupporting

confidence: 81%

Reduced Nasal Viral Load and IFN Responses in Infants with Respiratory Syncytial Virus Bronchiolitis and Respiratory Failure

Thwaites

Coates²,

Ito³

et al. 2018

Am J Respir Crit Care Med

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“…Moreover, false negatives are possible, especially when rapid antigen testing is used, (19, 20) if the specimen is obtained by a nasal swab rather than a nasal aspirate,(21) or if unsatisfactory specimens are obtained.…”

Section: Diagnostic Testingmentioning

confidence: 99%

Recent evidence on the management of bronchiolitis

Schroeder

Mansbach²

2014

Current Opinion in Pediatrics

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Purpose of Review Bronchiolitis is a common condition in children < 2 years and is a leading cause of infant hospitalization. While there is significant variability in testing and treatment of children with bronchiolitis, diagnostic testing rarely improves care, and no currently available pharmacologic options have been proven to provide meaningful benefit or improve outcomes. Recent Findings Beta-agonists continue to be used frequently despite evidence that they do not reduce hospital admissions or length-of-stay. In general, therapies initially considered promising were subsequently proven ineffective, a pattern seen in studies on corticosteroids, and more recently with nebulized racemic epinephrine and hypertonic saline. Recent research has improved our understanding of the viral epidemiology of bronchiolitis, with increasing recognition of viruses other than respiratory syncytial virus and better awareness of the role of viral co-infections. How these findings will translate into improved outcomes remains uncertain. Summary Much of the emphasis of the last few decades of bronchiolitis clinical care and research has centered on the identification and testing of novel therapies. Future quality improvement efforts should focus more on the limitation of unnecessary testing and treatments. Future research should include identification of subgroups of children with bronchiolitis that may benefit from focused clinical interventions.

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“…Although the sensitivity of the nasal swab is lower than that of the NPA, it is still used to detect mild cases of respiratory syncytial virus (RSV) infection, for community-based studies and surveillance purposes (Meerhoff et al, 2010). In the present study, the sensitivity of a salivary sample to detect the H1N1 virus was as good as that of the "classic" nasopharyngeal swab samples.…”

Section: Discussionmentioning

confidence: 76%

Salivary Detection of H1N1 Virus: A Clinical Feasibility Investigation

Bilder¹,

Machtei²,

Shenhar³

et al. 2011

J Dent Res

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The fast and efficient transportation among continents will continue to play a role in the spread of airborne pandemics. The objective of this study was to detect H1N1 virus in the saliva of individuals who visited the emergency department and were diagnosed as having H1N1 influenza. Nasopharyngeal swabs and saliva samples from those who presented to the emergency department with flu-like symptoms were sent to the laboratory. RNA was extracted from both samples. Real-time RT-PCR tests were performed, and the saliva and nasopharyngeal swab tests were compared. Samples were drawn from 26 individuals. A positive nasopharyngeal swab test and salivary test was found in 14 persons, and negative tests were found in 12 persons. Saliva sampling for H1N1 has excellent predictive value, is highly accurate and reliable, and is more convenient than the nasopharyngeal swab. Clinical trial with the Helsinki Committee at Rambam Health Care Campus, registration number 036309-RMB.

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Detection of multiple respiratory pathogens during primary respiratory infection: nasal swab versus nasopharyngeal aspirate using real-time polymerase chain reaction

Cited by 83 publications

References 30 publications

Reduced Nasal Viral Load and IFN Responses in Infants with Respiratory Syncytial Virus Bronchiolitis and Respiratory Failure

Reduced Nasal Viral Load and IFN Responses in Infants with Respiratory Syncytial Virus Bronchiolitis and Respiratory Failure

Recent evidence on the management of bronchiolitis

Salivary Detection of H1N1 Virus: A Clinical Feasibility Investigation

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