Detection of Mutant p53 Protein in Workers Occupationally Exposed to Benzidine

Cui-qin, Xiang; Shen, Chunlin; Wu, Zirong; Qin, Yi-qiu; Zhang, Yunying; Liu, Chazhen; Chen, Jigang; Zhang, Shengnian

doi:10.1539/joh.49.279

Cited by 14 publications

(3 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mutations of p53 also lead to change or even loss of p53 binding activity to its downstream genes, such as the mdm2 gene, resulting in aberrant cell proliferation and malignant cellular transformation . An abnormal expression of mutant p53 protein could lead to carcinogenesis of several major types of human cells . Thus, determination of the extent of p53 mutation is of great significance in clinical research and early diagnosis of cancers.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Simultaneous and Label-Free Determination of Wild-Type and Mutant p53 at a Single Surface Plasmon Resonance Chip Preimmobilized with Consensus DNA and Monoclonal Antibody

Wang

Zhu

et al. 2009

Anal. Chem.

View full text Add to dashboard Cite

Simultaneous determination of wild-type and total p53 proteins (wild-type and mutant combined) present in cancer cell lysates has been performed with a dual-channel surface plasmon resonance (SPR) instrument. To achieve specificity, each channel of the SPR chip was modified with a consensus double-stranded (ds-) DNA and a monoclonal antibody. The high affinity of the consensus ds-DNA to the wild-type p53 and the antibody to total p53 results in remarkably low detection levels (10.6 and 1.06 pM for the wild-type and total p53, respectively). The difference between the SPR signals reveals the extent of p53 mutation, which is indicative of cancer development. The SPR signals increase with the p53 concentration across a wide range (from low picomolar to nanomolar levels) that amply encompasses the typical cellular p53 concentrations. The applicability of the method to real sample analysis has been demonstrated with the comparative analyses of normal and cancer cell lysates. The normal cell samples all displayed significantly higher levels of wild-type p53. In contrast, elevated levels of mutant p53 were observed from the cancer cell lysates. In comparison with enzyme-linked immunosorbant assay (ELISA), SPR obviates the need of a second antibody labeled with an enzyme in the "sandwich enzyme immunoassay" format and is capable of real-time monitoring of the binding events. Thus, SPR could potentially serve as an attractive technique for rapid, sensitive, reliable, and label-free cancer diagnoses.

show abstract

mentioning

confidence: 99%

“…15 An abnormal expression of mutant p53 protein could lead to carcinogenesis of several major types of human cells. 16 Thus, determination of the extent of p53 mutation is of great significance in clinical research and early diagnosis of cancers.…”

mentioning

confidence: 99%

Simultaneous and Label-Free Determination of Wild-Type and Mutant p53 at a Single Surface Plasmon Resonance Chip Preimmobilized with Consensus DNA and Monoclonal Antibody

Wang

Zhu

et al. 2009

Anal. Chem.

View full text Add to dashboard Cite

show abstract

“…Subsequently, immuo-PCR is employed as a high-sensitivity detection method, taking advantage of the conjugates produced by the self-assembly of the STV, biotinylated dsDNA and antibodies directed against the protein. This assay allowed us to detect very low amounts of antigen, typically 100-1,000-fold less than is detectable by conventional sandwich ELISAs (34). In addition, this immunoassay can be performed in a single step by simultaneously tagging the protein with capture and detection reagents, thereby significantly reducing handling time.…”

Section: Discussionmentioning

confidence: 99%

The establishment of supramolecular immunobead real-time PCR and the identification of Cox-2 as a metastasis-related marker in colorectal carcinoma

Wang²,

Chang³

et al. 2012

Oncol Rep

View full text Add to dashboard Cite

Abstract. Cyclooxygenase-2 (Cox-2) is an inducible enzyme that converts arachidonic acid to prostaglandins, and it is hypothesized to induce carcinogenesis and metastasis in colorectal cancer. Our previous data also indicated that a higher expression level of Cox-2 was correlated with colorectal cancer metastasis. The Cox-2 protein was detected in the glandular cavity of colorectal cancer and the surrounding interstitial tissues. The usefulness of the Cox-2 gene as a gene therapy target and diagnostic marker remains unknown. In this study, a method using immuno-PCR and real-time PCR followed by supramolecular immunobead real-time PCR was established and used to detect the expression of Cox-2 in serum samples of nude mice with colorectal carcinoma. In addition, we established a Cox-2 gene stable knockdown colorectal cell line (SW480-EGFP-Cox-2 shRNA) using lentiviral vector-mediated RNA interference (RNAi) technology and established an imageable colorectal cancer metastasis mouse model. We found that the proliferation, invasion and tumorigenesis of SW480-EGFP-Cox-2 shRNA cells were attenuated compared with SW480 cells. In vivo experiments also demonstrated that angiogenesis in the Cox-2 knockdown colorectal cancer cells was decreased. The whole body optical imaging revealed that the SW480-EGFP-Cox-2 shRNA cells had an abrogated ability to develop metastases in the lymph nodes, lungs or liver in vivo. The improved immunobead PCR assay detected significantly lower Cox-2 protein levels in the serum samples of the SW480-EGFP-Cox-2 shRNA group compared with those of the SW480-EGFP-Cox-2-Ctrl shRNA group. In conclusion, our results indicated that the knockdown of Cox-2 expression suppressed the proliferation and invasion of colorectal cancer cells both in vitro and in vivo. This study also demonstrated that silencing Cox-2 in vivo reduced the metastastic potential of colorectal cancer. Thus, Cox-2 is a promising marker for the diagnosis of colorectal metastasis and a potential therapeutic target for colorectal cancer.

show abstract

Aromatic Amino and Nitro–Amino Compounds and Their Halogenated Derivatives

Darby

2023

Patty's Toxicology

View full text Add to dashboard Cite

The fundamental aromatic hydrocarbon benzene, when substituted by one or more functional groups of the amine, nitro, or halogen classes, alone or in various combinations, provides an enormous number of compounds of immense practical value, ubiquitous presence in the industrial world, and often heightened toxicity relative to benzene itself, a notorious cause of blood cancer. This chapter begins by providing a general overview of aromatic compounds possessing amino and/or nitro substituents, with or without halogen substituents. The production and uses, exposure and biomonitoring, toxicity, and toxic mechanism of action are introduced. The remainder of the chapter focuses on specific compounds in a roughly systematic order based upon chemical structure, expanding on the same subheadings as covered in the general overview. The nomenclature of these compounds can be very confusing since many have traditional names that sometimes markedly differ from their systematized formal names. In this chapter, the same traditional names are used as were used in the previous edition of this publication, but if there is ambiguity of nomenclature, the Chemical Abstracts Service (CAS) numbers provided herein correspond to unique structures, each of which may have several names depending upon the naming convention. In general, when an aromatic compound has an amino or a nitro substituent, it is referred to as an “aniline” or a “nitrobenzene,” respectively, based upon the names of the parent compounds, even though other substituents may be present. When amino and nitro groups are present on the same benzene ring, “aniline” takes precedence, and both “aniline” and “nitrobenzene” take precedence over halogens. There are many exceptions to these general rules, however.

show abstract

Detection of Mutant p53 Protein in Workers Occupationally Exposed to Benzidine

Cited by 14 publications

References 19 publications

Simultaneous and Label-Free Determination of Wild-Type and Mutant p53 at a Single Surface Plasmon Resonance Chip Preimmobilized with Consensus DNA and Monoclonal Antibody

Simultaneous and Label-Free Determination of Wild-Type and Mutant p53 at a Single Surface Plasmon Resonance Chip Preimmobilized with Consensus DNA and Monoclonal Antibody

The establishment of supramolecular immunobead real-time PCR and the identification of Cox-2 as a metastasis-related marker in colorectal carcinoma

Aromatic Amino and Nitro–Amino Compounds and Their Halogenated Derivatives

Contact Info

Product

Resources

About