Tuberculosis (TB) is a notable disease as well as a major global health problem; it is also one of the major infectious diseases in our country. Early diagnosis and treatment is a key step in the process of stopping Tuberculosis. In recent years, with the development of biotechnology, the Tuberculosis diagnosis methods have been fully improved, more and more new detection methods are constantly emerging, especially the molecular biological techniques for timely diagnosis of Tuberculosis and pathological research is of great significance. This article summarizes the main domestic detection techniques from the traditional bacteriological, immunological and molecular biological detection aspects, to provide some suggestions and ideas for medical staff and researchers.