Detection of Mycobacterial Antigen and Antibodies in the Cerebrospinal Fluid of Patients With Tuberculous Meningitis

Chandramuki, A; Allen, Patrick; Keen, Michael; Iványi, Juraj

doi:10.1099/00222615-20-2-239

Cited by 65 publications

(21 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition to measuring M. tuberculosisspecific antibodies or antibody-secreting cells, assays to measure M. tuberculosis-specific antigens directly in the CSF have also been evaluated (32,82,86,92,103,125,202,203,205,236) (Table 3). A theoretical advantage of antigen detection over antibody detection would be that they would be released only as a result of the host's immune response or the result of treatment.…”

Section: Molecular and Biochemical Analysismentioning

confidence: 99%

Central Nervous System Tuberculosis: Pathogenesis and Clinical Aspects

et al. 2008

View full text Add to dashboard Cite

SUMMARY Tuberculosis of the central nervous system (CNS) is a highly devastating form of tuberculosis, which, even in the setting of appropriate antitubercular therapy, leads to unacceptable levels of morbidity and mortality. Despite the development of promising molecular diagnostic techniques, diagnosis of CNS tuberculosis relies largely on microbiological methods that are insensitive, and as such, CNS tuberculosis remains a formidable diagnostic challenge. Insights into the basic neuropathogenesis of Mycobacterium tuberculosis and the development of an appropriate animal model are desperately needed. The optimal regimen and length of treatment are largely unknown, and with the rising incidence of multidrug-resistant strains of M. tuberculosis, the development of well-tolerated and effective antibiotics remains a continued need. While the most widely used vaccine in the world largely targets this manifestation of tuberculosis, the BCG vaccine has not fulfilled the promise of eliminating CNS tuberculosis. We put forth this review to highlight the current understanding of the neuropathogenesis of M. tuberculosis, to discuss certain epidemiological, clinical, diagnostic, and therapeutic aspects of CNS tuberculosis, and also to underscore the many unmet needs in this important field.

show abstract

Section: Molecular and Biochemical Analysismentioning

confidence: 99%

Central Nervous System Tuberculosis: Pathogenesis and Clinical Aspects

et al. 2008

View full text Add to dashboard Cite

show abstract

“…The assay detected antigen in 89% of culture-positive and in 73% of culture-negative cerebrospinal fluid samples from a total of 89 patients with clinically diagnosed tuberculous meningitis. False positives were seen in 21 % of pyogenic meningitis cases and in 8% of Indian and in 1 % of United Kingdom control cerebrospinal fluid samples [12]. The same group found cerebrospinal fluid antibody to the mycobacterial antigen in 68% of cases of tuberculous meningitis, but false positives were found in 37% of cases of pyogenic meningitis.…”

Section: Mycobacterial Meningitismentioning

confidence: 81%

“…Chandramuki and coworkers [12] developed a reverse passive hemagglutination assay utilizing an IgM monoclonal antibody to a soluble nonprotein mycobacterial antigen. The assay detected antigen in 89% of culture-positive and in 73% of culture-negative cerebrospinal fluid samples from a total of 89 patients with clinically diagnosed tuberculous meningitis.…”

Section: Mycobacterial Meningitismentioning

confidence: 99%

Cerebrospinal Fluid Infections

Graves¹

1989

The Cerebrospinal Fluid

View full text Add to dashboard Cite

The diagnosis of central nervous system infection, especially bacterial meningitis, remains the most compelling reason for lumbar puncture and examination of the cerebrospinal fluid. The traditional tests performed include the opening pressure, the protein and glucose concentrations, the total cell count and differential, and cultures of microbiological agents. These tests are obtained in virtually all cases of suspected central nervous system infection. The routine cerebrospinal fluid profile of cell count, protein, and glucose is an important guide to the diagnosis of noninfectious central nervous system disorders as well, including those of neoplastic, vascular, autoimmune, and degenerative etiologies. The routine availability of computed tomographic (CT) scanning and other imaging techniques has made it possible to visualize mass lesions rapidly and avoid lumbar puncture in many patients with increased intracranial pressure and risk of cerebral herniation.A number of newer tests of cerebrospinal fluid components beyond the routine profile are presently available, and others are being actively developed. A long list of enzymes, antigens, and ionic components can be determined on cerebrospinal fluid. In multiple sclerosis, studies of IgG and oligo clonal bands are now a major diagnostic aid. IgG studies are also of value in patients with autoimmune disease and chronic inflammatory and infectious disorders. In neoplastic disorders, a limited number of tumor markers and antigens may be presently measured on cerebrospinal fluid to guide diagnosis and treatment. The most important of the newer cerebrospinal fluid tests are those

show abstract

“…A cultura do Mycobacterium tuberculosis, apesar de representar a prova-ouro de confirmação diagnóstica, tem a grande limitação do tempo necessário para o crescimento do bacilo, que chega a 45 dias 3 . As técnicas mais modernas de confirmação diagnóstica, como a PCR (polymerase chain reaction), tem ainda o seu uso limitado a laboratórios de pesquisa clínica, não estando disponíveis na prática clínica diária 6 .…”

unclassified