Detection of Mycobacterium avium subsp. paratuberculosis in intestinal and lymph node tissues of water buffaloes (Bubalus bubalis) by PCR and bacterial culture

Pandian, Sivakumar; Tripathi, Bhupendra Nath; Singh, Neeraj Kumar

doi:10.1016/j.vetmic.2005.04.002

Cited by 67 publications

(55 citation statements)

References 23 publications

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“…High seroprevalence of JD average 29% (29.8% in cattle and 28.6% in buffalo) has been found in domestic animals by using indigenous, sensitive and MAP-specific ELISA kits in North India. The seroprevalence of JD in Uttar Pradesh (31.9%), Punjab (23.3%), Gujarat (13.39%) and Andhra Pradesh (16.26%) has been reported (Sivakumar et al, 2005;Tripathi, 2008;Singh et al, 2011;Mohan et al, 2013). In spite of very high morbidity rates and lower productivity, economic losses in production go unnoticed in India due to chronicity.…”

Section: Paratuberculosis (Or) Johne's Diseasementioning

confidence: 99%

Prevalence, diagnosis, management and control of important diseases of ruminants with special reference to indian scenario

Saminathan¹,

Rana²,

Ramakrishnan³

et al. 2016

JEBAS

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India possess huge livestock population, which is endangered by different endemic infectious diseases (bacterial, viral, protozoan and parasitic), which collectively causes significant economic losses to the landless poor farming community. Infectious diseases impose economic losses by causing morbidity, mortality, decreased production (milk, meat, wool etc.), decreased feed conversion ratio which results in reduced weight gain, decreased draught power and fertility. Furthermore, economic burden is also due to the cost of treatment, abortion, consequences on internal livestock movement, germplasm and international trade. In addition, some of the diseases are zoonotic and inflicts considerable impact on public health. Uncertain agrarian climate, unpredictable weather, drought, floods, migration of livestock, scarcity of fodders, and unhygienic zoo-sanitary and healthcare practices together resulted in endemicity of diseases ultimately leads to more incidence and prevalence of livestock and poultry diseases throughout the year. Synchronized monitoring and surveillance of disease throughout the country is a fundamental requirement for sustainable livestock production. With fairly developed telecommunication in India, following technologies like interactive voice response system, SMS through mobile/cell phones and toll-free landline phones (voice mail) are required for enhancing the effectiveness and efficiency of

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Section: Paratuberculosis (Or) Johne's Diseasementioning

confidence: 99%

Prevalence, diagnosis, management and control of important diseases of ruminants with special reference to indian scenario

Saminathan¹,

Rana²,

Ramakrishnan³

et al. 2016

JEBAS

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“…In a mega study by Singh et al, (2014a) based on multiple tests (microscopy, indigenous ELISA kit, culture and IS900 PCR), 28.3% average bio-load of MAP infection was reported in buffaloes from North India in last 28 years . Sivakumar et al (2005) reported similar high (70.0 and 30.0%) incidence in buffaloes from North India using PCR and culture. In another large study by Singh et al, (2008a) In another study, Ziehl-Neelsen"s stained tissue sections revealed acid-fast bacilli in grade-3 and grade-2 buffaloes and acid-fast granular debris were present in grade-1 buffalo.…”

Section: Discussionmentioning

confidence: 66%

Bio-incidence of bovine Johne’s disease in dairy buffaloes in Central and North India using sensitive goat based ‘indigenous elisa kit’ and traditional tests

Audarya¹,

Chhabra²,

Sahzad³

et al. 2016

JEBAS

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Present study was aimed to estimate the bio-incidence of Bovine Johne"s disease in native population of buffaloes (Murrah, Zaffarabadi and Bhadawari) in dairy farms and farmer"s herds located in Central and North India. Total 156 serum samples were screened from a dairy herd in Kiratpur (Malwa region), and 41.0% buffaloes were positive for Mycobacterium avium subsp. paratuberculosis (MAP) infection using "Indigenous ELISA kit". In another study, 101 young males (farmer"s herds) from the native tract (Jind / Rohtak, Haryana) of Murrah breed were screened and 80.1% were reported positive by kit. In a farm herd of Bhadawari buffaloes in its native tract (Etawah district in Uttar Pradesh) and dairy herd of Murrah buffaloes procured from native tract and kept in Mathura district of Uttar Pradesh, In the two farms 20.0% buffaloes were screened and 71.4% were positive by fecal Microscopy. Study reported high bio-incidence of BJD in native breeds of buffaloes in Malwa region of Central India, Etawah and Mathura (Uttar Pradesh) and Jind / Rohtak (Haryana) regions of North India, despite high slaughter rate, for meat harvesting. Goat based 'Indigenous ELISA kit' was employed for screening since commercial kits were costly for the screening of buffaloes. Moreover Imported 'ELISA kits' were based on PPA from 'Cattle Type' biotype', a different strain from other geographical regions / country. "Indigenous ELISA kit" using native 'Indian Bison type' biotype was efficient, specific and cost effective 'mass screening' test to estimate bio-incidence of BJD in large population of buffaloes at National level.

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“…Animais em estágios iniciais da enfermidade, geralmente apresentam lesões leves e localizadas principalmente nas porções de íleo, que com a evolução da enfermidade, se estende até o reto (Mota et al 2010, Dalto et al 2012. Exames histológicos e PCR em tecidos já foram realizados por Sivakumar et al (2005) e Mota et al (2010) em búfalos com paratuberculose e esses autores demonstraram que a pesquisa em amostras de tecido pode ser realizada e que apresenta resultados satisfatórios, assim como observado no presente trabalho. Com base nesses resultados, a biópsia retal mostrou-se eficiente para detecção de animais positivos e negativos em rebanhos onde existem casos de paratuberculose.…”

Section: Discussionunclassified

Diagnóstico de paratuberculose por biópsia retal em búfalos

et al. 2015

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Paratuberculosis in a herd of buffaloes was studied in the municipality of São Mateus, Maranhão, Brazil. Rectal biopsies were performed in 140 male and female Murrah, Mediterranean and crossbreed buffaloes older than 3 years. Postmortem examination of 11 buffaloes was performed to compare the rectal biopsies with possible lesions in mesenteric nodes and the intestine. The history of the herd and clinical examination revealed progressive weight loss and non-responsive antimicrobial diarrhea, dehydration and submandibular edema. Rectal biopsies showed in six buffaloes microscopically suggestive lesions for paratuberculosis through hematoxilin-eosin staining (HE), characterized by moderate multifocal granulomatous enteritis with epithelioid cell infiltration. In four buffaloes Langhans giant cells were found. In 15 buffaloes lymphocytic infiltrate was observed in the lamina propria of the large intestine. Ziehl-Neelsen staining (ZN) revealed in 4.3% (6/140) acid-fast bacilli in the rectal mucosa. Real time PCR amplified to 5.71% (7/140) Mycobacterium avium subsp. paratuberculosis (Map) DNA. 11 buffalos were submitted to postmortem examination, gross examination revealed augmented mesenteric nodes with whitish areas in the cut surface. The mucosa of the small intestine was irregular and thickened, with evident traverse folds and Peyer plates. The brownish intestinal content was fluid, the ileocecal valve area thickened and edematous with evident lymphatic vessels. Histological lesions in the mesenteric lymph node and small intestine four buffalo were compatible with those already described in the literature, and presented acid-fast bacilli by ZN staining and amplification of Map genetic material in qPCR. The concordance between the rectal biopsy and the postmortem samples was in agreement with the Kappa test (K=0.792) and was considered substantial or high. The rectal biopsy showed to be promising and can be used by practitioners, together with other techniques, for antemortem diagnosis in buffalo herds suspected to be affected by paratuberculosis. Samples obtained by rectal biopsy were suitable for ZN staining and real time PCR. Rectal biopsy can be used as a tool for diagnosis and control of Johne´s disease in buffaloes to select and eliminate positive animals within the herd and decrease gradually the spread of Map.

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Detection of Mycobacterium avium subsp. paratuberculosis in intestinal and lymph node tissues of water buffaloes (Bubalus bubalis) by PCR and bacterial culture

Cited by 67 publications

References 23 publications

Prevalence, diagnosis, management and control of important diseases of ruminants with special reference to indian scenario

Prevalence, diagnosis, management and control of important diseases of ruminants with special reference to indian scenario

Bio-incidence of bovine Johne’s disease in dairy buffaloes in Central and North India using sensitive goat based ‘indigenous elisa kit’ and traditional tests

Diagnóstico de paratuberculose por biópsia retal em búfalos

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