Detection of Mycoplasma bovis in Pneumonic Calves

Bovine respiratory disease (BRD) is a complex syndrome associated with high mortality in young calves and causes severe economic losses in the cattle industry worldwide. The current study investigated the prevalence and molecular characterization of common bacterial pathogens associated with respiratory symptoms in young calves from Sadat City, one of the largest industrial cities in Menoufiya Governorate, Egypt. In between December 2020 and March 2021, 200 mixed-breed young calves of 6–12 months were examined clinically. Of them, sixty (30%) calves showed signs of respiratory manifestations, such as coughing, serous to mucopurulent nasal discharges, fever, and abnormal lung sound. Deep nasal (Nasopharyngeal) swabs were collected from the affected calves for bacteriological investigation. Phenotypic characterization and identification revealed Mycoplasma bovis, Mycoplasma bovigenitalium, Pasteurella multocida, and Staphylococcus aureus in 8.33%, 5%, 5%, and 5% of the tested samples, respectively. The PCR technique using species-specific primer sets successfully amplified the target bacterial DNA in all culture-positive samples, confirming the identity of the isolated bacterial species. Partial gene sequencing of 16S rRNA gene of M. bovigenitalium, P. multocida, and S. aureus, and mb-mp 81 gene of M. bovis revealed high nucleotide similarity and genetic relationship with respective bacterial species reported from Egypt and around the world, suggesting transmission of these bacterial species between animal host species and localities. Our study highlights the four important bacterial strains associated with respiratory disorders in calves and suggests the possible spread of these bacterial pathogens across animal species and different geographic locations. Further studies using WGS and a large number of isolates are required to investigate the realistic lineage of Egyptian isolates and globally.

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“…A recent study recommended PCR as the principal molecular technique for detecting Mycoplasma spp. and M. bovis in pneumonic calves [44].…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Molecular Characterization of Mycoplasma Species, Pasteurella multocida, and Staphylococcus aureus Isolated from Calves with Respiratory Manifestations

Hashem

Mousa

Abdeen

et al. 2022

Animals

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“…The control positive in both PCR runs was supplied by Hamad et al . (2019a and 2019b ). Thermocycler programs were explained in Table 2 .…”

Section: Methodsmentioning

confidence: 97%

“…DNA has extracted from broilers’ specimens ( Kilic et al ., 2013 ; Hamad et al ., 2019a , 2019b ), for that 25 mg of each lung was cut and summed in the disposable container and kept at −80°C to be used later ( Santos et al ., 2010 ). DNA extraction kit was supplied by gSYNC™ Geneaid extraction kit, Korea.…”

Section: Methodsmentioning

confidence: 99%

“…For the diagnoses of MG by PCR technique, two pairs of PCR primers were used Table 1 and consisted of universal primer pair for the genus of Mycoplasma depending on 16S rRNA sequences and the second pair was specific for MG according to ( Aghabalaei and Hedaiati, 2012 ; Malekhoseini et al ., 2017 ), and the efficacy of these primers was confirmed by ( Hamad et al ., 2019a , 2019b ; Al-dabbagh et al ., 2021 ). primers were synthesized by Bioneer Co., Korea.…”

Section: Methodsmentioning

confidence: 99%

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Detection of Mycoplasma gallisepticum in broiler by PCR

Mahmmoud¹,

Hamad²,

Khudhur³

2022

Open Vet J

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Background: Mycoplasma is a significant microorganism of poultry, which can cause respiratory infections and synovial inflammation, bringing about huge financial misfortunes to poultry workmanship worldwide. Aim: The goal of existing research was to determine the infection rate of Mycoplasma gallisepticum (MG) from chronic respiratory disease (CRD) cases among broilers fields in Mosul/ Iraq using the polymerase chain reaction (PCR) technique. Methods: All 92 lungs samples were collected from broilers with classical respiratory signs in different regions of the Nineveh governorate for three months from February to April 2021.PCR tests were performed using two couple primers, one for the qualitative amplification of 16S rRNA genes (285 base pairs) in Mycoplasma spp. and the second couple for detection of M. gallisepticum (580 base pairs). Results: Among the samples obtained from broilers, 87 (94.7%) were positive for Mycoplasma, and 79 (85.9%) were positive for M. gallisepticum. Conclusion: Our results showed that MG infection in broiler chickens leads to serious clinical symptoms and severe lesions. The rate of Mycoplasma isolation in this study is high despite the short lifespan of broiler chickens.

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“…prebiotics, probiotics, vaccines, coccidiostats) have been major scientific achievements (Cangiano et al, 2020). As with calf diarrhoea, we have moved on from calf pneumonia being attributed to a "virus" (Maier et al, 2020) to polymerase chain reaction (PCR) tests for specific viruses and bacteria (Hamad et al, 2019). This achievement has allowed targeted respiratory vaccination protocols to be developed for young calves, even in the presence of maternally derived antibodies (Richeson & Falkner, 2020).…”

Section: Young Dairy Calf Healthmentioning

confidence: 99%

Ruminant health research – progress to date and future prospects, with an emphasis on Irish research

Mee

Barrett

Boloña

et al. 2022

Irish Journal of Agricultural and Food Research

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This review addresses the progress that has been made in ruminant health research over the last 60 yr, with an emphasis on Irish research. The review focuses on the economically important infectious diseases of dairy and beef cattle and of sheep, calf diseases, regulated and non-regulated infectious diseases, lameness, mastitis and parasitoses. The progress to date, current knowledge and future challenges are all addressed. Paradigm shifts have occurred in many of these diseases, the most profound of which is the change from increasing antimicrobial usage (AMU) to the realisation of the challenge of antimicrobial resistance (AMR) and the current reduction in AMU. Another major change in thinking is the move away from focus on the pathogen exclusively towards a more holistic view of the roles of host immunity and adequacy of management. In the last 60 yr, many new diseases have emerged but in parallel many new technologies have rapidly evolved to monitor and control these threats to animal health. Irish research has contributed substantially to improved current ruminant health. The major future challenge is how to manage ruminant health in a OneHealth world where animal, human and environmental health and sustainability are intimately intertwined and interdependent.

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Detection of Mycoplasma bovis in Pneumonic Calves

Cited by 8 publications

References 36 publications

Prevalence and Molecular Characterization of Mycoplasma Species, Pasteurella multocida, and Staphylococcus aureus Isolated from Calves with Respiratory Manifestations

Prevalence and Molecular Characterization of Mycoplasma Species, Pasteurella multocida, and Staphylococcus aureus Isolated from Calves with Respiratory Manifestations

Detection of Mycoplasma gallisepticum in broiler by PCR

Ruminant health research – progress to date and future prospects, with an emphasis on Irish research

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