Detection of Mycoplasma haemocanis, Mycoplasma haematoparvum, Mycoplasma suis and other vector-borne pathogens in dogs from Córdoba and Santa Fé, Argentina

Mascarelli, Patricia E.; Tartara, Gustavo P.; Pereyra, N. B.; Maggi, Ricardo G.

doi:10.1186/s13071-016-1920-8

Cited by 27 publications

(23 citation statements)

References 31 publications

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“…Evidences of infection with some of the pathogens studied here were found in other little populated areas, like in a range of jackal and fox species from northern Africa (Maia et al, 2014) and in arctic foxes (Mascarelli et al, 2016). In consequence, we cannot discard that the pathogens studied here were brought to Patagonia by dogs during the last two centuries.…”

Section: Discussionsupporting

confidence: 52%

“…Hemoplasma DNA was detected in some foxes and in a remarkable proportion of the flea pools analysed. Mycoplasma haemocanis infects dogs worldwide (Shapiro et al, 2017; Soto et al, 2017; Aktas and Ozubek, 2018), including Argentina (Mascarelli et al, 2016). As many as 77% of dogs from northern Argentina were infected with hemoplasmas; M. haemocanis was the most prevalent species (Mascarelli et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

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Assessing the natural circulation of canine vector-borne pathogens in foxes, ticks and fleas in protected areas of Argentine Patagonia with negligible dog participation

Millán

Travaini

Sacristán

et al. 2019

International Journal for Parasitology: Parasites and Wildlife

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We collected blood and/or ectoparasites from 49 South American grey foxes (Lycalopex griseus) and two Andean foxes (L. culpaeus) caught in two National Parks of southern Argentine Patagonia (Bosques Petrificados, BPNP; and Monte León, MLNP) where dogs are nearly absent (density < 0.01 dog/km2). Common ectoparasites were the flea Pulex irritans (88% prevalence) and the tick Amblyomma tigrinum (29%). Conventional PCR and sequencing of 49 blood samples, 299 fleas analysed in 78 pools, and 21 ticks revealed the presence of DNA of the following canine vector-borne pathogens: in grey foxes, Rickettsia sp. (3%), hemoplasmas (8%), including Mycoplasma haemocanis, and Hepatozoon sp. (50%); in P. irritans, Bartonella spp. (72% of flea pools from 76% of foxes), mostly B. vinsonii subsp. berkhoffii but also B. rochalimae, Anaplasmataceae (Wolbachia sp.; 60% and 54%), and M. haemocanis/haemofelis (29% and 18%); and in A. tigrinum, Hepatozoon sp. (33% of ticks in 4 of 7 foxes). No piroplasmid DNA was detected in any sample. Andean foxes were negative for all tested pathogens. Two different Hepatozoon haplotypes were detected: the most prevalent was phylogenetically associated with H. felis, and the other with H. americanum and related sequences. Amblyomma tigrinum and Hepatozoon sp. were more abundant and/or prevalent in BPNP than in colder MLNP, 300 km southwards, perhaps located close to the limit for tick suitability. Bartonella v. berkhoffii was also significantly more prevalent in fleas of foxes in BPNP than in MLNP. This study provides novel information about natural host-pathogen associations in wildlife, markedly extends the distribution area in South America of arthropods and vector-borne pathogens of veterinary and public health interest, and contributes preliminary evidence about the potential role of A. tigrinum and P. irritans as vectors, respectively, for potentially new species of Hepatozoon from Lycalopex spp. and for M. haemocanis that should be further investigated.

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Assessing the natural circulation of canine vector-borne pathogens in foxes, ticks and fleas in protected areas of Argentine Patagonia with negligible dog participation

Millán

Travaini

Sacristán

et al. 2019

International Journal for Parasitology: Parasites and Wildlife

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“…All dogs included in the Control Group (exposed, without CME signs) were negative both by PCR and the SPEED  EHRLI serological assay. These findings coincide with previous reports from other authors in which no DNA of E. canis was detected in samples obtained from clinically healthy dogs (Cicuttin et al 2015, Mascarelli et al 2016. However, some studies carried out in Buenos Aires city have reported a prevalence value of 13.5 for Anaplasma platys (Cicuttin et al 2011(Cicuttin et al , 2014a.…”

Section: Discussionsupporting

confidence: 91%

“…Cicuttin et al (2016) in samples of canines with symptoms compatible with EMC of the Metropolitan Area of Buenos Aires described a prevalence of 6.7% (15/223) to E. canis. On the other hand, in clinical healthy dogs from Buenos Aires, San Luis, Córdoba and Santa Fe, only the presence of Anaplasma platys DNA, but not E. canis, was demonstrated (Cicuttin et al 2011, 2014a, 2014b, 2017a, Mascarelli et al 2016. Serological studies published in our country are scarce.…”

Section: Introductionmentioning

confidence: 73%

Canine monocytic ehrlichiosis in Buenos Aires, Argentina: comparison of serological and molecular assays

Martín

Salvo²,

Cicuttin³

et al. 2019

Pesq. Vet. Bras.

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Canine monocytic ehrlichiosis (CME) is an infectious disease caused by the bacterium Ehrlichia canis and transmitted by Rhipicephalus sanguineus sensu lato, a tick with worldwide distribution. When not diagnosed and treated early, disease can be severe. Currently, the disease is confirmed by serological or molecular assays. The objective of this study was to compare a serological assay based on immunochromatography (SPEED® EHRLI immunochromatographic test; BVT, France) and a molecular assay (a screening PCR followed by a nested PCR specific for E. canis) for the diagnosis of E. canis in suspected dogs from Buenos Aires city and southern Greater Buenos Aires, Argentina. Blood samples from 20 clinically healthy dogs (Control Group) and from 80 sick dogs suspected of having CME (Groups 1 to 4) were tested in parallel. Neither the immunochromatographic test nor the PCR assay was able to detect the presence of E. canis in the Control Group. In the group which had been previously tested by serology, the agreement between the tests was low (kappa: 0.200), whereas in the group which had been previously tested by PCR, the concordance between the tests was adequate (kappa: 0.650). The concordance between the tests evaluated in the total population studied was moderate (kappa: 0.496). The results of our study suggest that the use of rapid serological tests as a first approach, together with subsequent confirmation by PCR, will improve the diagnosis of CME.

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“…Babesiosis due to B. vogeli has been described in three dogs from Buenos Aires [12, 251] and detected in 10% (2/21) and 6.8% (3/41) of shelter dogs from Córdoba and Santa Fé, respectively, by molecular methods [252]. Large piroplasms have furthermore been detected in 0.2% of tested animals in a large canine survey with more than 16,000 dogs [12, 251].…”

Section: Country Filesmentioning

confidence: 99%

A review on the occurrence of companion vector-borne diseases in pet animals in Latin America

2019

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Companion vector-borne diseases (CVBDs) are an important threat for pet life, but may also have an impact on human health, due to their often zoonotic character. The importance and awareness of CVBDs continuously increased during the last years. However, information on their occurrence is often limited in several parts of the world, which are often especially affected. Latin America (LATAM), a region with large biodiversity, is one of these regions, where information on CVBDs for pet owners, veterinarians, medical doctors and health workers is often obsolete, limited or non-existent. In the present review, a comprehensive literature search for CVBDs in companion animals (dogs and cats) was performed for several countries in Central America (Belize, Caribbean Islands, Costa Rica, Cuba, Dominican Republic, El Salvador, Guatemala, Honduras, Mexico, Nicaragua, Panama, Puerto Rico) as well as in South America (Argentina, Bolivia, Brazil, Chile, Colombia, Ecuador, French Guiana, Guyana (British Guyana), Paraguay, Peru, Suriname, Uruguay, Venezuela) regarding the occurrence of the following parasitic and bacterial diseases: babesiosis, heartworm disease, subcutaneous dirofilariosis, hepatozoonosis, leishmaniosis, trypanosomosis, anaplasmosis, bartonellosis, borreliosis, ehrlichiosis, mycoplasmosis and rickettsiosis. An overview on the specific diseases, followed by a short summary on their occurrence per country is given. Additionally, a tabular listing on positive or non-reported occurrence is presented. None of the countries is completely free from CVBDs. The data presented in the review confirm a wide distribution of the CVBDs in focus in LATAM. This wide occurrence and the fact that most of the CVBDs can have a quite severe clinical outcome and their diagnostic as well as therapeutic options in the region are often difficult to access and to afford, demands a strong call for the prevention of pathogen transmission by the use of ectoparasiticidal and anti-feeding products as well as by performing behavioural changes.

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Detection of Mycoplasma haemocanis, Mycoplasma haematoparvum, Mycoplasma suis and other vector-borne pathogens in dogs from Córdoba and Santa Fé, Argentina

Cited by 27 publications

References 31 publications

Assessing the natural circulation of canine vector-borne pathogens in foxes, ticks and fleas in protected areas of Argentine Patagonia with negligible dog participation

Assessing the natural circulation of canine vector-borne pathogens in foxes, ticks and fleas in protected areas of Argentine Patagonia with negligible dog participation

Canine monocytic ehrlichiosis in Buenos Aires, Argentina: comparison of serological and molecular assays

A review on the occurrence of companion vector-borne diseases in pet animals in Latin America

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