Detection of Mycoplasma hyopneumoniae by ELISA and nested PCR from blood samples and nasal swabs from pigs in Slovakia

Prokeš, Marián; Zendulková, D.; Rosenbergová, Kateřina; Treml, F.; Ondrejková, Anna; Benísek, Z; Ondrejka, R.; Korytár, Ľuboš; Slepecká, E.; Süli, Judit; Haladová, E.; Mazensky, David

doi:10.2754/avb201281040327

Cited by 3 publications

(1 citation statement)

References 18 publications

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“…Based on serological examination, piglets that were treated with antiparasitics showed substantially higher concentrations of specific antibodies (Prokeš et al 2012). The study confirmed that parasitic infection affects the efficacy of oral vaccination.…”

Section: Discussionsupporting

confidence: 57%

Does the infection with endoparasites influence the effect of oral vaccination against classical swine fever in wild boar?

et al. 2015

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Classical swine fever is a highly contagious viral disease that affects domestic and wild suids and could cause important economic losses. It is the most dangerous infectious disease of the wild boar that can cause severe death in densely populated areas. The aim of the study was to determine the effect of endoparasites on the oral vaccination against classical swine fever in wild boar. The study compared classical swine fever antibody titres in wild boar treated and untreated with antiparasitics. Fourteen six-month-old wild boar piglets were tested via direct ELISA to detect specific antibodies in blood serum after vaccination. Before the vaccination, one group of piglets was administered antiparasitic therapy; the other group of animals remained untreated. Twenty-eight days post vaccination, piglets from the first group (free of parasites) showed significantly (P = 0.0015) higher concentrations of specific antibodies than the infected animals. Obtained results proved that parasitic infections substantially influence the efficacy of oral vaccination against classical swine fever and may support the ability of the virus to produce infectious diseases and its transmission in the wild boar population. For that reason, antiparasitic therapy of wild boar populations before their vaccination is highly recommended in order to increase the vaccine's efficacy.

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Section: Discussionsupporting

confidence: 57%

Does the infection with endoparasites influence the effect of oral vaccination against classical swine fever in wild boar?

et al. 2015

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Sero-prevalence and associated risk factors of Mycoplasma hyopneumoniae infection in Kailali and Kanchanpur District of Far Western, Nepal

Acharya

Bhatta²,

Prajapati

et al. 2018

Comp Clin Pathol

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Heterologous prime-boost H1N1 vaccination exacerbates disease following challenge with a mismatched H1N2 influenza virus in the swine model

Pliasas,

Neasham,

Naskou

et al. 2023

Front. Immunol.

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Influenza A viruses (IAVs) pose a significant threat to both human and animal health. Developing IAV vaccine strategies able to elicit broad heterologous protection against antigenically diverse IAV strains is pivotal in effectively controlling the disease. The goal of this study was to examine the immunogenicity and protective efficacy of diverse H1N1 influenza vaccine strategies including monovalent, bivalent, and heterologous prime-boost vaccination regimens, against a mismatched H1N2 swine influenza virus. Five groups were homologous prime-boost vaccinated with either an oil-adjuvanted whole-inactivated virus (WIV) monovalent A/swine/Georgia/27480/2019 (GA19) H1N2 vaccine, a WIV monovalent A/sw/Minnesota/A02636116/2021 (MN21) H1N1 vaccine, a WIV monovalent A/California/07/2009 (CA09) H1N1, a WIV bivalent vaccine composed of CA09 and MN21, or adjuvant only (mock-vaccinated group). A sixth group was prime-vaccinated with CA09 WIV and boosted with MN21 WIV (heterologous prime-boost group). Four weeks post-boost pigs were intranasally and intratracheally challenged with A/swine/Georgia/27480/2019, an H1N2 swine IAV field isolate. Vaccine-induced protection was evaluated based on five critical parameters: (i) hemagglutination inhibiting (HAI) antibody responses, (ii) clinical scores, (iii) virus titers in nasal swabs and respiratory tissue homogenates, (iv) BALf cytology, and (v) pulmonary pathology. While all vaccination regimens induced seroprotective titers against homologous viruses, heterologous prime-boost vaccination failed to enhance HAI responses against the homologous vaccine strains compared to monovalent vaccine regimens and did not expand the scope of cross-reactive antibody responses against antigenically distinct swine and human IAVs. Mismatched vaccination regimens not only failed to confer clinical and virological protection post-challenge but also exacerbated disease and pathology. In particular, heterologous-boosted pigs showed prolonged clinical disease and increased pulmonary pathology compared to mock-vaccinated pigs. Our results demonstrated that H1-specific heterologous prime-boost vaccination, rather than enhancing cross-protection, worsened the clinical outcome and pathology after challenge with the antigenically distant A/swine/Georgia/27480/2019 strain.

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Detection of Mycoplasma hyopneumoniae by ELISA and nested PCR from blood samples and nasal swabs from pigs in Slovakia

Cited by 3 publications

References 18 publications

Does the infection with endoparasites influence the effect of oral vaccination against classical swine fever in wild boar?

Does the infection with endoparasites influence the effect of oral vaccination against classical swine fever in wild boar?

Sero-prevalence and associated risk factors of Mycoplasma hyopneumoniae infection in Kailali and Kanchanpur District of Far Western, Nepal

Heterologous prime-boost H1N1 vaccination exacerbates disease following challenge with a mismatched H1N2 influenza virus in the swine model

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