Detection of Mycoplasma-Like Organisms (Mlos) in Apricot by Fluorescence Microscopy (D.A.P.I.) in the South of Italy

Pastore, Mary P.; Marcone, Carmine; Pennone, F.; Cristinzio, Gennaro; Ragozzino, A.

doi:10.17660/actahortic.1995.386.72

Cited by 2 publications

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“…Morvan (1977) named the disease, which is associated with leptonecrosis ( Goidanich, 1934) or with new sprouting in winter, ‘apricot chlorotic leaf roll’ (ACLR). The association of these symptoms with a phytoplasma infection has been recognized for only 20 years, since phytoplasmas were detected as single cells in sieve tubes by electron or fluorescence microscopy, and transmission experiments to other stone fruits and indicator plants were successfully carried out ( Morvan, 1977; Goidanich et al ., 1980 ; Giunchedi et al ., 1982 ; Pastore et al ., 1995 ).…”

Section: Introductionmentioning

confidence: 99%

Presence of European stone fruit yellows (ESFY or 16SrX‐B) phytoplasmas in apricots in Austria

Machado¹,

Paltrinieri

Hanzer³

et al. 2001

Plant Pathology

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A severe decline of apricot trees, present in Austria for several years, has recently reached notifiable levels. Initial symptoms on affected trees resemble deficiencies in water and nutrient supplies, expressed as leaf rolling, chlorosis and early reddening, leading to sudden dieback during the growing season. Small, wilted fruit and dried leaves may also persist during the autumn. Phytoplasmas belonging to the 16SrX‐B subgroup of European stone fruit yellows (ESFY) were identified by nested polymerase chain reaction/restriction fragment length polymorphism analysis in a high percentage of affected trees from two different regions of Austria.

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Section: Introductionmentioning

confidence: 99%

Presence of European stone fruit yellows (ESFY or 16SrX‐B) phytoplasmas in apricots in Austria

Machado¹,

Paltrinieri

Hanzer³

et al. 2001

Plant Pathology

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Identification of Mycoplasma Contamination in Cell Culture

Тkachenko,

Kokovin,

Drozd

et al. 2023

Biological Systems: Theory and Innovation

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Mycoplasmas are the smallest and simplest prokaryotes wich were found in the endosomes of mammalian cells. They are widespread contaminants in cell cultures. It was identified a mycoplasma infection in a human breast cancer cell line. It was used fluorescence microscopy and nuclear affinity 4,6-diamidino-2-phenylindole (DAPI) staining. For the treatment of mycoplasma infection in cell culture, two antibiotics of the macrolide series (Tiamulin) and tetracyclines (Minocycline) was used. The effectiveness of combined antibiotic therapy against mycoplasmas has been proven, which was confirmed by the microscopic method. Therefore, treatment with combined antibiotics can completely eradicate mycoplasma infection from cultured cells.

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Detection of Mycoplasma-Like Organisms (Mlos) in Apricot by Fluorescence Microscopy (D.A.P.I.) in the South of Italy

Cited by 2 publications

References 0 publications

Presence of European stone fruit yellows (ESFY or 16SrX‐B) phytoplasmas in apricots in Austria

Presence of European stone fruit yellows (ESFY or 16SrX‐B) phytoplasmas in apricots in Austria

Identification of Mycoplasma Contamination in Cell Culture

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