Detection of Mycoplasmalike Organisms in Gladiolus Using Microscopy and Dna-Probes

Bertaccini, Assunta; Bellardi, M.G.; Vibio, M.; Lee, I.M.; Davis, Robert E.

doi:10.17660/actahortic.1992.325.101

Cited by 8 publications

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“…Symptomless plants collected in the field were used as negative controls. Similar samples of Brassica oleracea from Italy (Bertaccini et al ., 1994) were used to compare ribosomal and other uncharacterized chromosomal sequences. Isolates of an American aster yellows strain (AAY) obtained from E. Seemüller (Dossenheim, Germany), Maryland aster yellows (MAY) and clover phyllody (CPh) strains obtained from I.‐M.…”

Section: Methodsmentioning

confidence: 74%

“…Phytoplasmas infecting plants of the Brassica family were described and characterized by PCR amplification of the 16S rDNA gene fragment, RFLP analyses and dot‐hybridization in cabbage (Bertaccini et al ., 1990, 19921994; Marcone & Ragozzino, 1995a), sprouting broccoli (Bertaccini et al ., 1983; Marcone & Ragozzino, 1995a), turnip and kale (Marcone & Ragozzino, 1995a), and wild radish (Marcone & Ragozzino, 1995b).…”

Section: Discussionmentioning

confidence: 99%

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Comparison of phytoplasmas infecting winter oilseed rape in the Czech Republic with Italian Brassica phytoplasmas and their relationship to the aster yellows group

et al. 1998

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Winter oilseed rape grown in several areas in South Bohemia showed symptoms of stunting, leaf reddening and extensive malformation of floral parts. Phytoplasmas were consistently observed by using electron microscopy only in phloem tissue of symptomatic plants. DNA isolated from infected and healthy control plants was used in PCR experiments. Primer pairs R16F2/R2, P1/P7 and rpF2/R2, amplifying, respectively, 16S rDNA, 16S rDNA plus spacer region and the beginning of the 23S and ribosomal protein gene L22 specific for phytoplasmas, were used. According to RFLP and sequence analyses of PCR products, the phytoplasma from rape was classified in the aster yellows phytoplasma group, subgroup 16SrI-B. The PCR products from the Czech phytoplasma-infected rape also had RFLP profiles identical to those of phytoplasma strains from Italian Brassica. This first molecular characterization of phytoplasmas infecting rape compared with strains from Brassica does not, however, clearly indicate differences among isolates of the same 16SrI-B subgroup. Further studies on other chromosomal DNA portions could help the research on host specificity or on geographical distribution of these phytoplasmas.

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Section: Methodsmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Comparison of phytoplasmas infecting winter oilseed rape in the Czech Republic with Italian Brassica phytoplasmas and their relationship to the aster yellows group

et al. 1998

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“…Later, mycoplasma-like organisms (MLOs) were found to be associated with aster yellows symptoms, floral spiralization, malformed and virescent flowers in Gladiolus (Bertaccini and Marani 1980). MLOs in Gladiolus corms with fine symptoms have been detected using microscopy and DNA probes (Bertaccini et al 1990(Bertaccini et al , 1992, and PCR (Bertaccini et al 1994). In addition, aster yellows phytoplasma in Gladiolus was identified in Poland (Kamińska et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of a new isolate of phytoplasma associated with malformation and twisting of floral spikes of Gladiolus in India

et al. 2010

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In 2007In -2009, severe virescence, malformation and twisting of flower spikes and yellowing of entire plants were observed in various Gladiolus cultivars growing in the gardens of the National Botanical Research Institute, Lucknow, India. The disease symptoms were very similar to symptoms in Gladiolus caused by the Aster yellows phytoplasma identified from Poland. Disease incidence was low (1.1-3.4%), but the severity of symptoms was high. A phytoplasma infection was detected in nine of 13 cultivars by PCR followed by nested PCR using universal phytoplasma primers P1/P6 or R16F2n/R16R2, respectively. An amplicon of *1.2 kb obtained from the nested PCR was cloned and sequenced. Sequence analysis of the PCR amplicon revealed high (94-98%) identities and the closest phylogenic relationships with several isolates of Aster yellows phytoplasma of 'Candidatus Phytoplasma asteris' (16SrI group). Thus, the phytoplasma isolate of Gladiolus was identified as a new isolate of 'Ca. P. asteris' (16SrI group). In silico analysis of the phytoplasma isolate clearly indicated that the isolate was distinct from other Indian isolates of this phytoplasma.

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Phytoplasma Diseases in Ornamental Crops

Bellardi

Bertaccini

Madhupriya

et al. 2018

Phytoplasmas: Plant Pathogenic Bacteria - I

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Detection of Mycoplasmalike Organisms in Gladiolus Using Microscopy and Dna-Probes

Cited by 8 publications

References 0 publications

Comparison of phytoplasmas infecting winter oilseed rape in the Czech Republic with Italian Brassica phytoplasmas and their relationship to the aster yellows group

Comparison of phytoplasmas infecting winter oilseed rape in the Czech Republic with Italian Brassica phytoplasmas and their relationship to the aster yellows group

Molecular characterization of a new isolate of phytoplasma associated with malformation and twisting of floral spikes of Gladiolus in India

Phytoplasma Diseases in Ornamental Crops

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