Detection of natural infection in Lutzomyia cruzi and Lutzomyia forattinii (Diptera: Psychodidae: Phlebotominae) by Leishmania infantum chagasi in an endemic area of visceral leishmaniasis in Brazil using a PCR multiplex assay

Pita-Pereira, Daniela de; Cardoso, Maria Angélica; Alves, Carlos Roberto; Brazil, Reginaldo Peçanha; Britto, Constança

doi:10.1016/j.actatropica.2008.04.015

Cited by 90 publications

(65 citation statements)

References 19 publications

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“…En el período estudiado, el promedio anual fue de 40,6 casos de LVA humana y hubo un total de 27 óbitos (tasa de letalidad/año = 9,50%), siendo clasificado como municipio "de transmisión intensa" (promedio de casos humanos n ³ 4,4) por el 15 Ministerio de Salud de Brasil . Las localidades consideradas de transmisión intensa de LVA y en donde la abundancia relativa de Lu.…”

Section: Resultsunclassified

“…En Mato Grosso do Sul, en la región de Corumbá y Ladário, Lutzomyia cruzi (Mangabeira, 1938) y Lutzomyia forattinii (Galati, Rego, Nunes & Teruya, 1985) son las especies predominantes y simpátricas; ambas han sido consideradas 11 transmisoras del agente de la leishmaniasis visceral . Otra especie de la fauna flebotomínica de la Serra da Bodoquena, la Lutzomyia almerioi (Galati & Nunes, 1999), presentó infección natural por L. (L.) chagasi y Leishmania (Viannia) sp., alta densidad y acentuada antropofilia, sugestivas de la posibilidad de estar involucrada en la 12,13,14,15,16,17 transmisión de la enfermedad en esa región .…”

Section: Introductionunclassified

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Flebotomíneos (Diptera: Psychodidae: Phlebotominae) no Município de Três Lagoas, área de transmissão intensa de leishmaniose visceral, Estado de Mato Grosso do Sul, Brasil

et al. 2010

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Section: Resultsunclassified

Section: Introductionunclassified

Flebotomíneos (Diptera: Psychodidae: Phlebotominae) no Município de Três Lagoas, área de transmissão intensa de leishmaniose visceral, Estado de Mato Grosso do Sul, Brasil

et al. 2010

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“…Although these techniques are considered the gold standards, in the last two decades the development of PCR and other molecular biology assays have made testing in less-advanced laboratories practical and have enabled the use of highly sensitive diagnostic tools in areas where such diseases strongly impact the population. In particular, the usefulness of these techniques with respect to leishmaniasis lies in their ability to quickly identify Leishmania species, allowing for better surveillance of the vector directly involved in the transmission cycle (Cruz et al 2006, Moreira et al 2007, de Pita-Pereira et al 2008). …”

Section: Resultsmentioning

confidence: 99%

“…longipalpis sibling species (and proven vector of L. infantum) Lutzomyia cruzi (de Pita-Pereira et al 2008), respectively. Aside from differences in the sampling strategies and techniques used, the differences could be related to the eco-epidemiology of each urban VL foci.…”

Section: Resultsmentioning

confidence: 99%

Detection of Leishmania infantum in naturally infected Lutzomyia longipalpis (Diptera: Psychodidae: Phlebotominae) and Canis familiaris in Misiones, Argentina: the first report of a PCR-RFLP and sequencing-based confirmation assay

Acardi

Liotta

Santini

et al. 2010

Mem. Inst. Oswaldo Cruz

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In this study, a genotypification of Leishmaniawas performed using polimerase chain reaction-restriction fragment length polymorfism (PCR-RFLP) and sequencing techniques to identify species of Leishmaniaparasites in phlebotomine sand flies and dogs naturally infected. Between January-February of 2009, CDC light traps were used to collect insect samples from 13 capture sites in the municipality of Posadas, which is located in the province of Misiones of Argentina. Sand flies identified as Lutzomyia longipalpiswere grouped into 28 separate pools for molecular biological analysis. Canine samples were taken from lymph node aspirates of two symptomatic stray animals that had been positively diagnosed with canine visceral leishmaniasis. One vector pool of 10 sand flies (1 out of the 28 pools tested) and both of the canine samples tested positively for Leishmania infantumby PCR and RFLP analysis. PCR products were confirmed by sequencing and showed a maximum identity with L. infantum. Given that infection was detected in one out of the 28 pools and that at least one infected insect was infected, it was possible to infer an infection rate at least of 0.47% for Lu. longipalpisamong the analyzed samples. These results contribute to incriminate Lu. longipalpis as the vector of L. infantumin the municipality of Posadas, where cases of the disease in humans and dogs have been reported since 2005

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“…such as the quantitative polymerase chain reaction (qPCR), nested PCR, and multiplex PCR (20)(21)(22)(23)(24)(25)(26)(27)(28). The possibility of combining multiple targets in the same assay led to expansion of applications of the multiplex PCR technique on leishmaniasis diagnosis, since the identification of complexes and species of the parasite to evaluation of sample integrity and PCR performance on sandfly pools (23)(24)(25)(26)(27)(28).…”

Section: Introductionmentioning

confidence: 99%

Application of the mammalian glyceraldehyde-3-phosphate dehydrogenase gene for sample quality control in multiplex PCR for diagnosis of leishmaniasis

Gonçalves¹,

Régis-da-Silva²,

Brito³

et al. 2012

J. Venom. Anim. Toxins incl. Trop. Dis

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Leishmaniasis is a neglected disease endemic in five continents. It is a severe disease that may lead to death, and its early detection is important to avoid severe damage to affected individuals. Molecular methods to detect Leishmania are considered alternatives to overcome the limitations presented by conventional methods. The aim of this study was to develop multiplex PCR systems able to detect small amounts of target DNA of Leishmania infantum and Leishmania braziliensis, and the gene coding for glyceraldehyde-3-phosphate dehydrogenase (G3PD) in mammals, enabling quality evaluation of the sample simultaneously with detection of the specific target. The systems created for G3PD recognition were combined with detection systems for L. infantum and L. braziliensis to compose multiplex PCR systems for visceral (mVL) and cutaneous (mACL) leishmaniasis diagnosis. The multiplex PCR systems developed were assessed in blood samples from five different species of mammal reservoirs involved in the disease cycle in Brazil, and 96 and 52 human samples from patients with suspected visceral leishmaniasis (VL) and cutaneous leishmaniasis (ACL), respectively. Three G3PD detection systems were created (G3PD1, G3PD2 and G3PD3) with different product sizes, G3PD2 was chosen for the formation of multiplex PCR systems. The two multiplex PCR systems (mVL and mACL) were reproducible in all species evaluated. Results of test samples (sensitivity, specificity and efficiency) suggest its use in routine diagnosis, research activities in medicine and veterinary medicine. Additionally, the systems designed to detect the G3PD gene are capable of combining with other targets used for molecular diagnosis of infectious diseases. Concerning leishmaniasis, the multiplex PCR systems can be used in epidemiological studies for the detection of new and classic reservoirs, which may contribute to the reliability of results and development of actions to control the disease.

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Detection of natural infection in Lutzomyia cruzi and Lutzomyia forattinii (Diptera: Psychodidae: Phlebotominae) by Leishmania infantum chagasi in an endemic area of visceral leishmaniasis in Brazil using a PCR multiplex assay

Cited by 90 publications

References 19 publications

Flebotomíneos (Diptera: Psychodidae: Phlebotominae) no Município de Três Lagoas, área de transmissão intensa de leishmaniose visceral, Estado de Mato Grosso do Sul, Brasil

Flebotomíneos (Diptera: Psychodidae: Phlebotominae) no Município de Três Lagoas, área de transmissão intensa de leishmaniose visceral, Estado de Mato Grosso do Sul, Brasil

Detection of Leishmania infantum in naturally infected Lutzomyia longipalpis (Diptera: Psychodidae: Phlebotominae) and Canis familiaris in Misiones, Argentina: the first report of a PCR-RFLP and sequencing-based confirmation assay

Application of the mammalian glyceraldehyde-3-phosphate dehydrogenase gene for sample quality control in multiplex PCR for diagnosis of leishmaniasis

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