Detection of neoplastic lymphocytes in peripheral blood of dogs with lymphoma by polymerase chain reaction for antigen receptor gene rearrangement

Keller, Rachel L.; Avery, Anne C.; Burnett, Robert; Walton, Judy A.; Olver, Christine S.

doi:10.1111/j.1939-165x.2004.tb00364.x

Cited by 41 publications

(32 citation statements)

References 3 publications

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“…4 More recently, a similar assay was developed based on the canine genome database by targeting the T-cell receptor gamma chain (TCRc) gene 15 and the immunoglobulin heavy chain (IgH) gene. 9 Although the diagnostic value of PCR analysis has been demonstrated in several types of lymphoproliferative disorders, 7,[11][12][13][14] only limited studies have been done to identify neoplastic proliferation of lymphocytes in dogs with small-cell lymphocytosis. In the present study, immunophenotypical evaluation was performed on the blood of dogs with small lymphocytosis.…”

Section: Introductionmentioning

confidence: 99%

Immunophenotyping and Gene Rearrangement Analysis in Dogs with Lymphoproliferative Disorders Characterized by Small-Cell Lymphocytosis

Yagihara

Uematsu²,

Koike³

et al. 2009

J VET Diagn Invest

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Abstract. Lymphocytosis caused by neoplastic proliferation of small lymphocytes is occasionally difficult to distinguish by morphological examination from nonneoplastic lymphocytosis. To examine the clinical utility of gene rearrangement analysis for demonstrating neoplastic proliferation of small lymphocytes, gene rearrangement analysis was performed in comparison with immunophenotyping using peripheral lymphocytes in dogs with small lymphocytosis. Thirty-one dogs with small-cell lymphocytosis (8,100-884,300/ml) were enrolled. By immunophenotyping, lymphocytosis of all dogs was suggested to be neoplastic in nature based on the detection of marked expansion of phenotypically homogeneous lymphocytes or the presence of an aberrant antigen-expressing population of lymphocytes. In contrast, gene rearrangement analysis represented clonality in 27 dogs (detection rate of 87%). From the present study, gene rearrangement analysis was considered to be worthwhile to strengthen the evidence of neoplastic proliferation of small lymphocytes when coupled with immunophenotyping and to be a suitable diagnostic substitute if immunophenotyping is not available in clinical practice.

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Section: Introductionmentioning

confidence: 99%

Immunophenotyping and Gene Rearrangement Analysis in Dogs with Lymphoproliferative Disorders Characterized by Small-Cell Lymphocytosis

Yagihara

Uematsu²,

Koike³

et al. 2009

J VET Diagn Invest

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“…So PARR is an objective and highly sensitive method to detect clonal populations of B or T lymphocytes (Burnett et al, 2003). The sensitivity of PARR for detecting lymphoma cells may provide a noninvasive method for detecting lymphoma in its early stages, determining phenotype, monitoring therapy, screening breeds at risk for lymphoma, and possibly for assessing prognosis (Keller et al, 2004). Also, this method can be applied for the diagnosis of lymphoma from limited biopsy samples, formalin-fixed/paraffin-embedded tissues (Wan et al, 1990;Tai and Peh, 2003), or even sections already mounted on slides and stained (Fend et al, 1999;Yu et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…The stage of canine lymphoma is currently diagnosed primarily by morphological assessment of lymphoid tissue based on subjective lightmicroscopic assessments (Keller et al, 2004;Tamura et al, 2006). Although this definition of stage V lymphoma appears easy, microscopic criteria are subjective and varied to detect neoplastic cells in lymph node and peripheral blood.…”

Section: Discussionmentioning

confidence: 99%

“…According to a study, the rate of leukemic involvement of peripheral blood ranges from 28% to 65% in dogs with multicentric lymphoma, and depends on the stringency of criteria used (Keller et al, 2004). Thus, the assessment of clonal rearrangements of antigen receptor genes using PCR has been introduced into veterinary medicine, recently (Vernau and Moore, 1999;Burnett et al, 2003;Keller et al, 2004;Tamura et al, 2006;Yagihara et al, 2007). Also, in Korean veterinary clinics, diagnosis of canine B cell or T cell lymphoma using PCR was often carried out in case of uncertain if it is polyclonal or monoclonal lymphocytes.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of polymerase chain reaction for antigen receptor gene rearrangement and flow cytometric analysis for the diagnosis of canine lymphoma

Song

Yu²,

Kim³

et al. 2011

Korean Journal of Veterinary Service

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Lymphoma is the most common hematopoietic malignancy in dogs. Diagnosis of lymphoma is classically performed by morphological assessment and immunohistochemistry. But some cases in the early stage are difficult to distinguish and need more objective and accurate methods. So, Polymerase chain reaction (PCR) for antigen receptor rearrangements (PARR) and flow cytometric immunophenotype of lymphoma have been developed continuously. In this study, we performed these two methods to classify lymphoma type in 3 cases. According to PARR analysis, B cell origin lymphoma was diagnosed in two of three cases by testing PBMC and lymph node. All fine needle aspiration (FNA) samples of lymph nodes had high expression of CD21 on ＞88% of total cell population and PBMC samples also showed high expression of CD21 on ＞30% of total lymphocytes in those two cases, while the expression of CD3, CD4 and CD8 was absent. These results suggest that concurrent use of PARR and flow cytometric immunophenotype is more effective and valuable tool for the diagnosis and monitoring of canine lymphoma patients.

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“…In PARR, clonal expansion of tumor lymphocytes that contain a rearranged immunoglobulin heavy chain (IgH) gene or a T cell receptor gamma (TCR) gene can be detected in B-cell and T-cell lymphoid neoplasias, respectively. PARR is an objective and highly sensitive method, and its usefulness in diagnosis of canine lymphoid neoplasia has recently been proven [3,8,10,17]. In the present study, we evaluated the usefulness of PARR using endoscopic biopsy specimens in diagnosis of alimentary lymphoma.…”

mentioning

confidence: 99%

Application of Polymerase Chain Reaction to Analysis of Antigen Receptor Rearrangements to Support Endoscopic Diagnosis of Canine Alimentary Lymphoma

Kaneko

Yamamoto

Wada

et al. 2009

The Journal of Veterinary Medical Science

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ABSTRACT. We evaluated the usefulness of polymerase chain reaction for antigen receptor gene rearrangement analysis (PARR) of endoscopic biopsy specimens for diagnosis of canine alimentary lymphoma. Two endoscopic biopsy specimens were obtained from each lesion in 78 dogs with gastrointestinal symptoms. One specimen was histopathologically examined by a pathologist, and the other was analyzed by PARR. All samples were categorized into three groups [lymphoma (n=4), adenocarcinoma (n=5) and enteritis groups (n=69)] based on the histopathological diagnosis. In the lymphoma group, one case was IgH major-positive, and three cases were TCR-positive, representing clonal expansion of B-and T-cells, respectively. PARR produced negative results for all cases in the adenocarcinoma group. In the enteritis group, six cases were TCR-positive. Two of the six TCR-positive enteritis cases were cytologically diagnosed as lymphoma by fine needle aspiration during a laparotomy. In the enteritis group, the survival times were compared between the TCR-positive and TCR-negative cases. The overall survival time of the TCR-positive enteritis cases was significantly shorter than that of the TCR-negative enteritis cases according to a log-rank test (p<0.0001). With regard to other factors, such as age, clinical signs and the serum albumin concentration, there were no significant differences between the TCR-positive and TCR-negative enteritis cases. In conclusion, PARR is capable of detecting alimentary lymphoma and latent alimentary lymphoma, which cannot be histopathologically diagnosed using endoscopic biopsy specimens. Furthermore, a TCR-positive result in PARR may imply a poor prognosis.

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Detection of neoplastic lymphocytes in peripheral blood of dogs with lymphoma by polymerase chain reaction for antigen receptor gene rearrangement

Cited by 41 publications

References 3 publications

Immunophenotyping and Gene Rearrangement Analysis in Dogs with Lymphoproliferative Disorders Characterized by Small-Cell Lymphocytosis

Immunophenotyping and Gene Rearrangement Analysis in Dogs with Lymphoproliferative Disorders Characterized by Small-Cell Lymphocytosis

Comparison of polymerase chain reaction for antigen receptor gene rearrangement and flow cytometric analysis for the diagnosis of canine lymphoma

Application of Polymerase Chain Reaction to Analysis of Antigen Receptor Rearrangements to Support Endoscopic Diagnosis of Canine Alimentary Lymphoma

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