Detection of new candidate genes controlling seed weight by integrating gene coexpression analysis and QTL mapping in Brassica napus L.

Dong, Hongli; Yang, Lei; Liu, Yilin; Tian, Guifu; Tang, Huan; Xin, Shuangshuang; Cui, Yixin; Xiong, Qing; Wan, Heng; Li, Zhi; Jung, Christian; Qian, Wei

doi:10.1016/j.cj.2022.09.009

Cited by 2 publications

(2 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Members of the SCPL class have been identified and characterized in wheat ( Triticum aestivum ) (Xu et al ., 2021), rapeseed ( Brassica napus ) (Liu et al ., 2022), grape ( Vitis vinifera ) (Wang et al ., 2021), cotton ( Gossypium hirsutum ) (Wang et al ., 2022) and tea ( Camellia sinensis ) (Ahmad et al ., 2020). Previous studies have ascertained that SCPLs play essential functions in determining seed weight and size (Dong et al ., 2023; Li et al ., 2011), signal transduction (Xu et al ., 2015), development and senescence of organs (Cercós et al ., 2003; Domínguez et al ., 2002), plant abiotic stress responses (Xu et al ., 2021) and plant disease resistance (Mugford et al ., 2009; Wang et al ., 2022). For instance, a SCPL protein GS5 positively regulates grain size by promoting mitosis in Oryza sativa (Li et al ., 2011).…”

Section: Discussionmentioning

confidence: 99%

“…For instance, a SCPL protein GS5 positively regulates grain size by promoting mitosis in Oryza sativa (Li et al ., 2011). In rapeseed, SCPL19 influences seed weight through abscisic acid signalling (Dong et al ., 2023). In cotton, GhSCPL42 plays a crucial role in resistance to Verticillium wilt through its involvement in the salicylic acid and jasmonic acid signalling pathways (Wang et al ., 2022).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A 5.2‐kb insertion in the coding sequence of PavSCPL, a serine carboxypeptidase‐like enhances fruit firmness in Prunus avium

Qi,

Dong,

Liu

et al. 2024

Plant Biotechnology Journal

View full text Add to dashboard Cite

SummaryFruit firmness is an important trait in sweet cherry breeding because it directly positively influences fruit transportability, storage and shelf life. However, the underlying genes responsible and the molecular mechanisms that control fruit firmness remain unknown. In this study, we identified a candidate gene, PavSCPL, encoding a serine carboxypeptidase‐like protein with natural allelic variation, that controls fruit firmness in sweet cherry using map‐based cloning and functionally characterized PavSCPL during sweet cherry fruit softening. Genetic analysis revealed that fruit firmness in the ‘Rainier’ × ‘Summit’ F1 population was controlled by a single dominant gene. Bulked segregant analysis combined with fine mapping narrowed the candidate gene to a 473‐kb region (7418778–7 891 914 bp) on chromosome 6 which included 72 genes. The candidate gene PavSCPL, and a null allele harbouring a 5244‐bp insertion in the second exon that completely inactivated PavSCPL expression and resulted in the extra‐hard‐flesh phenotype, were identified by RNA‐sequencing analysis and gene cloning. Quantitative RT‐PCR analysis revealed that the PavSCPL expression level was increased with fruit softening. Virus‐induced gene silencing of PavSCPL enhanced fruit firmness and suppressed the activities of certain pectin‐degrading enzymes in the fruit. In addition, we developed functional molecular markers for PavSCPL and the Pavscpl5.2‐k allele that co‐segregated with the fruit firmness trait. Overall, this research identified a crucial functional gene for fruit firmness. The results provide insights into the genetic control and molecular mechanism of the fruit firmness trait and present useful molecular markers for molecular‐assisted breeding for fruit firmness in sweet cherry.

show abstract