Detection of new hosts for white spot syndrome virus of shrimp using nested polymerase chain reaction

Hossain, Md. Shahadat; Chakraborty, Anirban; Joseph, Biju; Otta, Subhendu Kumar; Karunasagar, Indrani

doi:10.1016/s0044-8486(00)00571-8

Cited by 95 publications

(66 citation statements)

References 27 publications

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“…At least 18 cultured and/or wild penaeid shrimp (Park et al, 1998), eight caridean species (Pramod-Kiran et al, 2002), seven species of lobster (Rajendran et al, 1999), seven species of crayfish (Edgerton, 2004), 38 crab species (Yoganandhan et al, 2003) six non-decapod crustacean species (Hossain et al, 2001), members of the phyla Chaetognata and Rotifera (Yan et al, 2004), polychaete worms (Supak et al, 2005) and some aquatic insect larva (Ramírez-Douriet et al, 2005) have been found susceptible to the virus. Histopathological observations during WSV infection in brief is available regarding shrimp species such as Penaes monodon (Durand et al, 1997;Wang et al, 1999Wang et al, , 2000Mishra and Shekhar, 2005) Litopenaeus vannamei and Marsupenaeus japonicus (Lu et al, 1997;Lightner et al, 1998;Escobedo-Bonilla et al, 2007;Perez et al, 2005;Pantoja and Lightner, 2003), species of Crabs (Kanchanaphum et al, 1998;Kou et al, 1998) and lobster (Rajendran et al, 1999;Wang et al, 1998;Jiravanichpaisal et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Pathological changes in Fenneropenaeus indicus experimentally infected with white spot virus and virus morphogenesis

Manjusha

Varghese

Philip

et al. 2009

Journal of Invertebrate Pathology

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a b s t r a c tTo demonstrate pathological changes due to white spot virus infection in Fenneropenaeus indicus, a batch of hatchery bred quarantined animals was experimentally infected with the virus. Organs such as gills, foregut, mid-gut, hindgut, nerve, eye, heart, ovary and integument were examined by light and electron microscopy. Histopathological analyses revealed changes hitherto not reported in F. indicus such as lesions to the internal folding of gut resulted in syncytial mass sloughed off into lumen, thickening of hepatopancreatic connective tissue with vacuolization of tubules and necrosis of rectal pads in hindgut. Virus replication was seen in the crystalline tract region of the compound eye and eosinophilic granules infiltrated from its base. In the gill arch, dilation and disintegration of median blood vessel was observed. In the nervous tissues, encapsulation and subsequent atrophy of hypertrophied nuclei of the neurosecretory cells were found. Transmission electron microscopy showed viral replication and morphogenesis in cells of infected tissue. De novo formed vesicles covered the capsid forming a bilayered envelop opened at one end inside the virogenic stroma. Circular vesicles containing nuclear material was found fused with the envelop. Subsequent thickening of the envelop resulted in the fully formed virus. In this study, a correlation was observed between the stages of viral multiplication and the corresponding pathological changes in the cells during the WSV infection. Accordingly, gill and foregut tissues were found highly infected during the onset of clinical signs itself, and are proposed to be used as the tissues for routine disease diagnosis.

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Section: Introductionmentioning

confidence: 99%

Pathological changes in Fenneropenaeus indicus experimentally infected with white spot virus and virus morphogenesis

Manjusha

Varghese

Philip

et al. 2009

Journal of Invertebrate Pathology

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“…Within a decade, it has become a serious threat to the shrimp culture industry throughout Asia and Latin America (Hill 2002). WSSV also infects many other crustacean species from several regions of the world (Lo et al 1996, Chang et al 1998, Kasornchandra et al 1998, Wang et al 1998, Rajendran et al 1999, Corbel et al 2001, Hossain et al 2001, Sahul-Hameed et al 2003.…”

Section: Introductionmentioning

confidence: 99%

Standardized white spot syndrome virus (WSSV) inoculation procedures for intramuscular or oral routes

Escobedo-Bonilla¹,

Audoorn²,

Wille³

et al. 2006

Dis. Aquat. Org.

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In the past, strategies to control white spot syndrome virus (WSSV) were mostly tested by infectivity trials in vivo using immersion or per os inoculation of undefined WSSV infectious doses, which complicated comparisons between experiments. In this study, the reproducibility of 3 defined doses (10, 30 and 90 shrimp infectious doses 50% endpoint [SID 50 ]) of WSSV was determined in 3 experiments using intramuscular (i.m.) or oral inoculation in specific pathogen-free (SPF) Litopenaeus vannamei. Reproducibility was determined by the time of onset of disease, cumulative mortality, and median lethal time (LT 50 ). By i.m. route, the 3 doses induced disease between 24 and 36 h post inoculation (hpi). Cumulative mortality was 100% at 84 hpi with doses of 30 and 90 SID 50 and 108 hpi with a dose of 10 SID 50 . The LT 50 of the doses 10, 30 and 90 SID 50 were 52, 51 and 49 hpi and were not significantly different (p > 0.05). Shrimp orally inoculated with 10, 30 or 90 SID 50 developed disease between 24 and 36 hpi. Cumulative mortality was 100% at 108 hpi with doses of 30 and 90 SID 50 and 120 hpi with a dose of 10 SID 50 . The LT 50 of 10, 30 and 90 SID 50 were 65, 57 and 50 hpi; these were significantly different from each other (p < 0.05). A dose of 30 SID 50 was selected as the standard for further WSSV challenges by i.m. or oral routes. These standardized inoculation procedures may be applied to other crustacea and WSSV strains in order to achieve comparable results among experiments.

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“…This further suggests that Japanese shrimp also have long history of association with WSSV with the viral gene segments inserted into the host chromosome. WSSV can often be detected in wild shrimp tested in many parts of the world [3,8,12,23], but this has not caused any significant decline in natural populations of these shrimp as evident from capture fisheries data from Asia since the mid nineties [6]. It is possible that aquaculture systems provided the environment in which WSSV outbreaks could be triggered.…”

Section: Disease Transmission Risk From Shrimps Exported For Human Comentioning

confidence: 99%

Shrimp Viral Diseases, Import Risk Assessment and International Trade

Karunasagar

Ababouch

2012

Indian J. Virol.

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Shrimp is an important commodity in international trade accounting for 15 % in terms of value of internationally traded seafood products which reached $102.00 billion in 2008. Aquaculture contributes to over 50 % of global shrimp production. One of the major constraints faced by shrimp aquaculture is the loss due to viral diseases like white spot syndrome, yellow head disease, and Taura syndrome. There are several examples of global spread of shrimp diseases due to importation of live shrimp for aquaculture. Though millions of tonnes of frozen or processed shrimp have been traded internationally during the last two decades despite prevalence of viral diseases in shrimp producing areas in Asia and the Americas, there is no evidence of diseases having been transmitted through shrimp imported for human consumption. The guidelines developed by the World Animal Health Organisation for movement of live animals for aquaculture, frozen crustaceans for human consumption, and the regulations implemented by some shrimp importing regions in the world are reviewed.

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Detection of new hosts for white spot syndrome virus of shrimp using nested polymerase chain reaction

Cited by 95 publications

References 27 publications

Pathological changes in Fenneropenaeus indicus experimentally infected with white spot virus and virus morphogenesis

Pathological changes in Fenneropenaeus indicus experimentally infected with white spot virus and virus morphogenesis

Standardized white spot syndrome virus (WSSV) inoculation procedures for intramuscular or oral routes

Shrimp Viral Diseases, Import Risk Assessment and International Trade

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