Detection of new SHV-12, SHV-5 and SHV-2a variants of extended spectrum Beta-lactamase in Klebsiella pneumoniae in Egypt

Newire, Enas; Ahmed, Salwa F.; House, Brent; Valiente, Esmeralda; Pimentel, Guillermo

doi:10.1186/1476-0711-12-16

Cited by 31 publications

(29 citation statements)

References 21 publications

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“…Alignment of the sequences with wild type of SHV-5, SHV-12 and SHV-1 in E. coli and K. pneumoniae isolates on the GenBank with accession numbers (E. coli NC_004998.1, E. coli NC_024967.1 and K. pneumoniae X98098.1), respectively, was 90-100% ( Figure 1). This matched with Newire et al [30], who explained that comparison of the corresponding amino acid sequence of the 11 SHV positive isolates demonstrated the predominance of SHV-12, while SHV-5 and SHV-2a was the most closely related sequencing match in the remaining isolates. SHV-12 is thought to have evolved from SHV-2a, and SHV-5 from SHV-2.…”

Section: Concerningsupporting

confidence: 86%

Molecular Characterization of β-lactamase Genes in Antibiotic Resistant Bacteria

Attia

Hassan

Elwan

2017

Zagazig Veterinary Journal

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Beta-lactamases (bls) are implicated seriously in resistance to β -lactam antibiotics. β-lactam resistance related to Sulfa hydral variabl-beta lactamases (SHV-bls) were detected in E. coli and Klebsiellae isolated from human and chickens. In this work, resistance profile of the isolates, extended spectrum β-lactamases (ESBLs) activity and SHV genes sequencing were examined. E. coli was isolated from humans, broiler and day-old chicks samples, meanwhile, K. pneumonia was isolated only from human samples (88.8%). Sixty-four (88.8 %) of human isolates contained betalactamases (iodometric positive), 28% were ESBLs and 35% contained SHV. In case of day-old chick isolates, 50% contained beta-lactamases (iodometric positive), 5.5% were ESBLs. Out of 45 broiler isolates, 37.7% contained beta-lactmases (iodometric positive), 8.8% were ESBLs and 80% contained SHV. It was found that SHV were of SHV-5 and SHV-12. It could be concluded that, wide spread of ESBLs SHV-5 and SHV-12 in humans and chicken is a major concern in resistance to β -lactam drugs.

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Section: Concerningsupporting

confidence: 86%

Molecular Characterization of β-lactamase Genes in Antibiotic Resistant Bacteria

Attia

Hassan

Elwan

2017

Zagazig Veterinary Journal

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“…However, there exists some reports that tend to suggest that TEM-1 may also contribute to the ESBL trait in concert with other β-lactamases. 7,40 Upon DNA sequencing of PCR products from all strains with bla group genes, the distribution of the β-lactamase subtypes corresponded with the M-PCR results. CTX-M-14 and CTX-M-3 were the most prevalent among the ESBL-producing clinical K. pneumoniae and E. coli strains.…”

Section: Discussionmentioning

confidence: 85%

Development of a multiplex PCR system and its application in detection of blaSHV, blaTEM, blaCTX-M-1, blaCTX-M-9 and blaOXA-1 group genes in clinical Klebsiella pneumoniae and Escherichia coli strains

et al. 2015

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Resistance to β-lactam antibiotics through β-lactamase production by Enterobacteriaceae continues to burden the health-care sector worldwide. Traditional methods for detection of β-lactamases are time-consuming and labor-intensive and newer methods with varying capabilities continue to be developed. The objective of this study was to develop a multiplex PCR (M-PCR) system for the detection of bla SHV , bla TEM , bla CTX-M-1 , bla CTX-M-9 and bla OXA-1 group genes and to apply it in clinical Klebsiella pneumoniae and Escherichia coli strains. To do this, we used group-specific PCR primers in singleplex reactions followed by optimization into multiplex reactions. Specificity and sensitivity of the M-PCR were then evaluated using 58 reference strains before its application to detect bla group genes in 203 clinical Enterobacteriaceae strains. PCR amplicons were sequenced to determine the β-lactamase subtypes. The M-PCR system exhibited 100% specificity and sensitivity. In all, 83.7% of K. pneumoniae and 89.8% of E. coli clinical strains harbored bla group genes with 46.9%, 40.1%, 15.0%, 21.1% and 6.1% of K. pneumoniae having bla SHV , bla TEM , bla CTX-M-1 , bla CTX-M-9 and bla OXA-1 group genes, respectively, whereas 12.2%, 77.6%, 22.4%, 36.7% and 8.2% of E. coli had bla SHV , bla TEM , bla CTX-M-1 , bla CTX-M-9 and bla OXA-1 group genes, respectively. Bla SHV-1 , bla SHV-11 , bla SHV-27 , bla SHV-33 , bla SHV-144 , bla TEM-1 , bla TEM-135 , bla OXA-1 , bla CTX-M-3 , bla CTX-M-9 , bla CTX-M-14 , bla CTX-M-15 , bla CTX-M-27 , bla CTX-M-55 , bla CTX-M-65 and bla CTX-M-104 were detected. In conclusion, the M-PCR system was efficient and versatile with an advantage of simultaneously detecting all the targeted bla group genes. Hence, it is a potential candidate for developing M-PCR kits for the screening of these genes for clinical or epidemiological purposes.

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“…K. pneumoniae isolates carrying multiple b-lactamase genes have been previously reported in other studies. 13,[43][44][45] Four isolates coexpressed bla CTX-M-15 along with other ESBLs. Three isolates harbored both SHV-5 and CTX-M-15, and only one isolate coproduced both SHV-12 and CTX-M-15.…”

Section: Discussionmentioning

confidence: 98%

“…9,10 The clonal dissemination of CTX-M-15-producing K. pneumoniae isolates has been reported in Saudi Arabia, Egypt, Iran, Oman, Lebanon, Gulf Council, Iraq, Kuwait, and Turkey. 9,[11][12][13][14][15][16][17][18][19] No data are available, so far, on ESBL-producing K. pneumoniae in Gaza Strip, Palestine and very scarce data in West Bank, Palestine. There have been reports of bla CTX-M-15 , bla CTX-M-56 , bla OXA-1 , bla SHV-1 , bla SHV-12 , bla SHV-32 , and bla TEM-1 genes in Gram-negative bacteria isolated from Palestinian patients in West Bank.…”

Section: Introductionmentioning

confidence: 99%

First Report of Extended-Spectrum β-Lactamases Among Clinical Isolates ofKlebsiella pneumoniaein Gaza Strip, Palestine

Tayh

SallemRym

YahiaHoussem

et al. 2017

Microbial Drug Resistance

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Sixteen broad-spectrum cephalosporin-resistant Klebsiella pneumoniae isolates were recovered between April and June 2013 from Palestinian hospitals, Gaza Strip. Genes encoding extended-spectrum beta-lactamases (ESBLs) and other resistance genes were characterized by polymerase chain reaction and sequencing. The following β-lactamase genes were detected: bla+ bla+ bla (six isolates), bla+ bla+ bla (two isolates), bla (two isolates), bla+ bla (one isolate), bla+ bla (one isolate), bla+ bla+ bla(one isolate), bla+ bla (one isolate), bla+ bla (one isolate), and bla (one isolate). The ISEcp1 (in four cases truncated by IS26), orf477, or IS903 sequences were found upstream or downstream of bla genes. The aac(6')-Ib-cr gene was found in seven isolates. The qnrS1 and qnrB1 genes were detected in five isolates and two isolates, respectively. Seven isolates contained class 1 integrons with four gene cassette arrangements: dfrA5 (three isolates), dfrA12-orf-aadA2 (two isolates), dfrA17-aadA5 (one isolate), and aadA1 (one isolate). A high clonal diversity was also observed among studied isolates by pulsed-field gel electrophoresis (12 unrelated profiles). This study demonstrates for the first time the emergence and polyclonal spread of multidrug-resistant ESBL-producing K. pneumoniae isolates among patients in a hospital setting in Gaza Strip, Palestine.

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Detection of new SHV-12, SHV-5 and SHV-2a variants of extended spectrum Beta-lactamase in Klebsiella pneumoniae in Egypt

Cited by 31 publications

References 21 publications

Molecular Characterization of β-lactamase Genes in Antibiotic Resistant Bacteria

Molecular Characterization of β-lactamase Genes in Antibiotic Resistant Bacteria

Development of a multiplex PCR system and its application in detection of blaSHV, blaTEM, blaCTX-M-1, blaCTX-M-9 and blaOXA-1 group genes in clinical Klebsiella pneumoniae and Escherichia coli strains

First Report of Extended-Spectrum β-Lactamases Among Clinical Isolates ofKlebsiella pneumoniaein Gaza Strip, Palestine

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