Detection of non-dairy fat in milk based on quantitative assay of anionic detergent using azure A dye

Barui, Amit Kumar; Sharma, Rajan; Rajput, Y. S.

doi:10.1016/j.idairyj.2011.11.007

Cited by 27 publications

(16 citation statements)

References 8 publications

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“…2). The labolene is laboratory grade detergent having about 21 % (w/w) active ingredients (Barui et al 2012) while purity of SDBS was nearly 100 % (w/w). It appears that absence or presence of diffused spot is dependent on the concentration of active ingredients in commercial or analytical preparation.…”

Section: Resultsmentioning

confidence: 99%

“…Detergent is believed to be one of the essential components of such milk-like preparation and is foreign to pure milk (Paradkar et al 2000a(Paradkar et al , b, 2001Sadat et al 2006). Detergent is added to emulsify foreign fat from non-milk origin, which is added as a substitute for milk fat (Paradkar et al 2001;Barui et al 2012). Anionic detergents are used most widely in such milk-like preparations due to their low cost and ease in availability (Barui et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Detergent is added to emulsify foreign fat from non-milk origin, which is added as a substitute for milk fat (Paradkar et al 2001;Barui et al 2012). Anionic detergents are used most widely in such milk-like preparations due to their low cost and ease in availability (Barui et al 2012). Several attempts have been made to detect anionic detergent in such milk samples.…”

Section: Introductionmentioning

confidence: 99%

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A rapid paper chromatographic method for detection of anionic detergent in milk

et al. 2013

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A paper chromatographic method for the detection of adulteration of anionic detergent in milk is described. The method is based on the complexing of anionic detergent with methylene blue dye and separation of complex from free dye using simple paper chromatographic method. Since complexing of detergent is with dye, visualization is direct without involvement of subsequent detection of complex. The method is simple and results are available in 10 min. The method is sensitive to detect 0.1 % (w/v) labolene (laboratory grade detergent) or 0.01 % (w/v) sodium dodecylbenzene sulfonate (pure anionic detergent) in milk. The method can be adopted at quality control laboratories in dairies for ascertaining the quality of milk.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A rapid paper chromatographic method for detection of anionic detergent in milk

et al. 2013

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“…It is well-known staining dye, used to stain blood smear and also in electrochemical biosensing [21]. In a report, azure A is employed as an agent who is capable of detecting the anionic detergents present in milk [22].…”

Section: Thioninementioning

confidence: 99%

Spectroscopic Studies of the Binding Interactions of Phenothiazininum Dyes (Thionine Acetate, Azure A and Azure B) with Calf-thymus DNA

Kumar¹,

Kaushik²

2016

J Drug Metab Toxicol

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The double helical structure of DNA offers various binding sites for the interaction of ligands or proteins. Interactions using minor groove, major groove, and through intercalation are the major types of binding mechanisms of DNA-ligand interactions. The lowering in the absorption intensity along with bathochromic shift is the indication of intercalation binding mode of the dye into the base pairs of the DNA. In this study, the interaction of phenothiazine dyes with calf-thymus DNA (ctDNA) in physiological buffer (pH 7.4) was studied using UV-visible, fluorescence, circular dichroism (CD), and UV-thermal denaturation spectroscopy. The binding constants were calculated at different temperatures with the help of fluorescence spectroscopy. CD signals signify that B-form of DNA might become more compact, upon binding of the dyes. Also, induced circular dichroism is observed which confirms the dye-DNA complex formation. Stabilization of DNA double helix upon binding with dyes was confirmed by the increase in T m of ctDNA. Based on thermal melting profiles, it was found that thionine acetate is most promising in stabilizing the DNA double helix, in comparison to other two dyes. Also, binding constants calculated by fluorescence is in accordance with the thermal melting analysis. These results are indicative of the intercalation binding mode between dyes and the DNA. The binding affinity of the dyes to DNA is found to be in order as thionine acetate > azure A > azure B. Such preliminary studies facilitate our understanding about various types of DNAligand interactions and provide clues for designing new and more effective drugs.

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“…Na adulteração do teor de gordura, têm sido empregados gordura animal e óleos vegetais, com a adição de surfactantes aniônicos que são utilizados na emulsificação [61,62].…”

Section: Introductionunclassified

Desenvolvimento de procedimentos analíticos explorando microextração líquido-líquido em fluxo para a determinação de espécies de interesse em águas e leite

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Detection of non-dairy fat in milk based on quantitative assay of anionic detergent using azure A dye

Cited by 27 publications

References 8 publications

A rapid paper chromatographic method for detection of anionic detergent in milk

A rapid paper chromatographic method for detection of anionic detergent in milk

Spectroscopic Studies of the Binding Interactions of Phenothiazininum Dyes (Thionine Acetate, Azure A and Azure B) with Calf-thymus DNA

Desenvolvimento de procedimentos analíticos explorando microextração líquido-líquido em fluxo para a determinação de espécies de interesse em águas e leite

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