Detection of norovirus (GI, GII), Sapovirus and astrovirus in fecal samples using reverse transcription single-round multiplex PCR

Aetiology, epidemiology and clinical characteristics of acute moderate-to-severe diarrhoea in children under 5 years of age hospitalized in a referral paediatric hospital in Rabat, Morocco

“…Detection and genotyping of rotavirus was performed following the procedures of Rodríguez-Díaz et al (2009). Detection of sapovirus, norovirus and astrovirus was carried out using the primers described by Yan et al (2003) with a multiplex Reverse transcriptase (RT)-PCR using the standard conditions described in a commercial kit (Superscript III one step RT-PCR; Invitrogen, Genome Biotechnologies). The presence of hepatitis A was established in a monoplex RT-PCR as described elsewhere (Sánchez et al 2002).…”

Development of RT-multiplex PCR Assay for Detection of Adenovirus and Group A and C Rotaviruses in Diarrheal Fecal Specimens from Children in China

“…Therefore, a rapid and sensitive assay is preferred to screen for the presence of these viruses in diarrheal fecal specimens. In a previous study, we developed a reverse transcription single-round multiplex polymerase chain reaction (RT-smPCR) assay for the simultaneous detection of norovirus (genogroup I, genogroup II), sapovirus and astrovirus in fecal specimens (Yan et al, 2003). Recently, we developed another RT multiplex PCR for one-step amplification of all subgenera A to F adenoviruses, and group A and C rotaviruses.…”

Protein Expression of the Human Norovirus Capsid Gene using the Baculovirus Expression System

“…A stool suspension of approximately 10% was prepared in Dulbecco's Phosphate Buffered Saline (DPBS) using the selected human norovirus positive sample (HuNoV GII/4 positive sample) and precleared by centrifugation at 13,000 rpm for 15 min at 4℃ (7,8). Genomic RNA was extracted from stool supernatant using the QIAamp Viral RNA mini kit (Qiagen), according to the manufacturer's protocol.…”