Detection of nosemosis in European honeybees (Apis mellifera) on honeybees farm at Kanchanaburi, Thailand

Abstract

This study was aimed to the detection of Nosema in European honeybees at Kanchanaburi Province and Identify species of Nosema by Polymerase Chain Reaction technique. The ventriculus of bees was individually checked to verify the presence of Nosema spores under light microscope. The number of spores per bee were quantify on a haemocytometer for infectivity. It was studied for three periods of the year. The first period was studied between October 2015 to January 2016, the second period from February to May 2016 and the third period from June to September 2016. The results showed that the highest infection rate in June-September 2019 was 100 and the lowest infection rate was 40 in October 2015 and January 2019. The average number of Nosema spores infection level in an individual was infectivity. The highest infectivity was 2.47x106 spores/bee in February to May 2016 and the lowest infectivity was 0.04x106 spores/bee in October 2015 to January 2016. Nosema ceranae was found in this study by Polymerase Chain Reaction technique.