2014
DOI: 10.3325/cmj.2014.55.259
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Detection of novel auto-antigens in patients with recurrent miscarriage: description of an approach and preliminary findings

Abstract: AimTo develop and test a protocol for isolation of potential auto-antigens from chorionic tissue that may be linked to recurrent miscarriage (RM).MethodsThe strategy included: 1) isolation of IgGs tightly bound to chorionic tissue of RM patients by protein G chromatography; 2) construction of affinity columns using the chorionic antibodies for isolation of auto-antigens; 3) enrichment of auto-antigens from detergent extracted solution of chorionic proteins by affinity chromatography; 4) separation by dodecyl s… Show more

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Cited by 9 publications
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