Detection of novel ferret coronaviruses and evidence of recombination among ferret coronaviruses

Minami, Shohei; Kuroda, Yudai; Terada, Yutaka; Yonemitsu, Kenzo; Nguyen, Dung Van; Kuwata, Ryusei; Shimoda, Hiroshi; Takano, Ai; Maeda, Ken

doi:10.1007/s11262-016-1365-3

Cited by 14 publications

(19 citation statements)

References 17 publications

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“…Thus far, most of the recombination events of coronaviruses have been reported between species of the same group (Herrewegh et al, 1998;Keck et al, 1988), such as among the batassociated CoVs (Corman et al, 2014) and 229E-related CoVs (Corman et al, 2015), and the major recombination breakpoint has mainly been within the S gene. Genetic recombinant among alphacoronavirus 1 species frequently occurred, such as ferret coronaviruses (Lamers et al, 2016;Minami et al, 2016). However, few researches about genetic recombination of Betacoronavirus 1 species have been conducted.…”

Section: Discussionmentioning

confidence: 99%

Discovery of a novel canine respiratory coronavirus support genetic recombination among betacoronavirus1

Wang

Chen

et al. 2017

Virus Research

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Although canine respiratory coronavirus (CRCoV) is an important respiratory pathogen that is prevalent in many countries, only one complete genome sequence of CRCoV (South Korea strain K37) has been obtained to date. Genome-wide analyses and recombination have rarely been conducted, as small numbers of samples and limited genomic characterization have previously prevented further analyses. Herein, we report a unique CRCoV strain, denoted strain BJ232, derived from a CRCoV-positive dog with a mild respiratory infection. Phylogenetic analysis based on complete genome of all available coronaviruses consistently show that CRCoV BJ232 is most closely related to human coronavirus OC43 (HCoV-OC43) and BCoV, forming a separate clade that split off early from other Betacoronavirus 1. Based on the phylogenetic and SimPlot analysis we propose that CRCoV-K37 was derived from genetic recombination between CRCoV-BJ232 and BCoV. In detail, spike (S) gene of CRCoV-K37 clustered with CRCoV-BJ232. However orf1ab, membrane (M) and nucleocapsid (N) genes were more related to Bovine coronavirus (BCoV) than CRCoV-B232. Molecular epidemic analysis confirmed the prevalence of CRCoV-BJ232 lineage around the world for a long time. Recombinant events among Betacoronavirus 1 may have implications for CRCoV transmissibility. All these findings provide further information regarding the origin of CRCoV.

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Section: Discussionmentioning

confidence: 99%

Discovery of a novel canine respiratory coronavirus support genetic recombination among betacoronavirus1

Wang

Chen

et al. 2017

Virus Research

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“…Recombination in coronaviruses plays an important role in virus evolution and can result in the emergence of new pathotypes (Menachery, Graham, & Baric, ; Wang et al, ) as well as changing the host ranges and ecological niches (Bakkers et al, ). Thus far, recombination regions in coronaviruses have been extensively reported for the S gene (Kin et al, ; Lau et al, ; Minami et al, ), a finding also applicable to BCoV (Martínez et al, ). Recombination events in M (Herrewegh, Smeenk, Horzinek, Rottier, & Groot, ), N (Kin et al, ), RP3 (Lau et al, ) and the ORF1 gene (Chen et al, ; Kin et al, ) have also been reported.…”

Section: Introductionmentioning

confidence: 93%

Prevalence of a novel bovine coronavirus strain with a recombinant hemagglutinin/esterase gene in dairy calves in China

Keha

Xue

Shen

et al. 2019

Transbound Emerg Dis

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Bovine coronavirus (BCoV) is the causative agent of diarrhoea in newborn calves, winter dysentery in adult cattle and respiratory tract illnesses in cattle across the world. In this study, a total of 190 faecal samples from dairy calves with diarrhoea were collected from 14 farms in six Chinese provinces, and BCoV was detected in 18.95% (36/190) of the samples by reverse transcriptase polymerase chain reaction. Full‐length spike, hemagglutinin/esterase (HE), nucleocapsid and transmembrane genes were simultaneously cloned from 13 clinical samples (eight farms in four provinces), and most of the BCoV strains showed a unique evolutionary pattern based on the phylogenetic analysis of these genes. Interesting, 10 of the 13 strains were identified as HE recombinant strains, and these strains had experienced the same recombination event and carried the same recombination sites located between the esterase and lectin domain. They also shared an identical aa variant (F181V) in the R2‐loop. Moreover, 9/10 strains displayed another identical aa variant (P, S158A) in the adjacent R1‐loop of the HE gene, which differs from the other available BCoV HE sequences in the GenBank database. Our results showed that BCoV is widely circulating in dairy cattle in China, contributing to the diagnosis and control of dairy calves diarrhoea. Furthermore, a BCoV strain that carries a recombinant HE gene has spread in dairy calves in China. To the best of our knowledge, this is the first description of an HE recombination event occurring in BCoV; this is also the first description of the molecular prevalence of BCoV in China. Our findings will enhance current understanding about the genetic evolution of BCoV.

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“…Recombination in coronaviruses plays an important role in virus evolution and can result in the emergence of new pathotypes [ 7 ]. Thus far, recombination regions in coronaviruses have been extensively reported for the S gene [ 8 ]. BCoV is transmitted via the fecal-oral or respiratory route [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Coronavirus and Rotavirus Associated with Calves in Central Part of Oromia, Ethiopia

Seid¹,

Dawo

Tesfaye

et al. 2020

Veterinary Medicine International

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Background. Coronavirus and rotavirus are most commonly associated etiologies for calves’ diarrhoea, resulting in loss of productivity and economy of farmers. However, various facets of diarrheal disease caused by coronavirus and rotavirus in calves in Ethiopia are inadequately understood. A cross-sectional study was conducted with the aim of isolation and molecular characterization of coronavirus and rotavirus from calves in the central part of Oromia (Bishoftu, Sebata, Holeta, and Addis Ababa), Ethiopia, from November 2018 to May 2019. The four study areas were purposively selected and faecal samples were collected by simple random sampling for diagnosis of coronavirus and rotavirus infection by using the antigen detection enzyme-linked immunosorbent assay (Ag-ELISA) kit. In addition, this study was carried out to have insight in prevalence and associated risk factors of coronavirus and rotavirus infection in calves. Result. During the study, 83 diarrheic and 162 nondiarrheic faecal samples collected from calves less than 4 weeks of age were screened for coronavirus and rotavirus. Of the 83 diarrheic samples, 1 sample (1.2%) was positive for coronavirus antigen and 6 samples (7.2%) were found to be positive for rotavirus antigen by Ag-ELISA. All the nondiarrheic samples were negative for both coronavirus and rotavirus Ag. The overall prevalence of coronavirus and rotavirus infection in calves was estimated at 0.4% (1/245) and 2.45% (6/245), respectively. All samples (7) of ELISA test positive of both coronavirus and rotavirus were propagated in Madin-Darby bovine kidney (MDBK) cells. After 3 subsequent passages, progressive cytopathic effect (CPE), i.e., rounding, detachment, and the destruction of monolayer cell of five samples (1 sample of coronavirus and 4 samples of rotavirus) (71.4%) were observed. At the molecular stage, reverse transcriptase polymerase chain reaction (RT-PCR) technique was used to determine the presence of coronavirus and rotavirus nucleic acid by using specific primers. The 5 samples that were coronavirus and rotavirus antigen positive by ELISA and develop CPE on cell culture were also positive on RT-PCR technique. The prevalence of infection peaked at 1st and 2nd weeks of age in male calves. Conclusion. Diarrheal disease caused by coronavirus and rotavirus has a great health problem in calves that interrupts production benefits with reduced weight gain and increased mortality and its potential for zoonotic spread. So, the present findings show coronavirus and rotavirus infection in calves in Ethiopia that needs to be addressed by practising early colostrum feeding in newborn calves, using vaccine, or improving livestock management.

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Detection of novel ferret coronaviruses and evidence of recombination among ferret coronaviruses

Cited by 14 publications

References 17 publications

Discovery of a novel canine respiratory coronavirus support genetic recombination among betacoronavirus1

Discovery of a novel canine respiratory coronavirus support genetic recombination among betacoronavirus1

Prevalence of a novel bovine coronavirus strain with a recombinant hemagglutinin/esterase gene in dairy calves in China

Isolation and Characterization of Coronavirus and Rotavirus Associated with Calves in Central Part of Oromia, Ethiopia

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