Detection of novel QTLs for foxglove aphid resistance in soybean

Abstract

The Raso2 , novel QTL for Korea biotype foxglove aphid resistance in soybean from PI 366121 was identified on chromosome 7 using GoldenGate SNP microarray. Foxglove aphid, Aulacorthum solani (Kaltenbach), is a hemipteran insect that infects a wide variety of plants worldwide and causes serious yield losses in crops. The objective of this study was to identify the putative QTL for foxglove aphid resistance in wild soybean, PI 366121, (Glycine soja Sieb. and Zucc.). One hundred and forty-one F4-derived F8 recombinant inbred lines developed from a cross of susceptible Williams 82 and PI 366121 were used. The phenotyping of antibiosis and antixenosis resistance was done through choice and no-choice tests with total plant damage and primary infestation leaf damage; a genome-wide molecular linkage map was constructed with 504 single-nucleotide polymorphism markers utilizing a GoldenGate assay. Using inclusive composite interval mapping analysis for foxglove aphid resistance, one major candidate QTL on chromosome 7 and three minor QTL regions on chromosomes 3, 6 and 18 were identified. The major QTL on chromosome 7 showed both antixenosis and antibiosis resistance responses. However, the minor QTLs showed only antixenosis resistance response. The major QTL mapped to a different chromosome than the previously identified foxglove aphid resistance QTL, Raso1, from the cultivar Adams. Also, the responses to the Korea biotype foxglove aphid were different for Raso1, and the gene from PI 366121 against the Korea biotype foxglove aphid was different. Thus, the foxglove aphid resistance gene from PI 366121 was determined to be an independent gene from Raso1 and was designated as Raso2. This result could be useful in breeding for new foxglove aphid-resistant soybean cultivars.

Mapping quantitative trait loci controlling soybean seed starch content in an interspecific cross of ‘Williams 82’ (Glycine max) and ‘PI 366121’ (Glycine soja)

“…A total of 501 polymorphic SNP markers (Lee et al. ) and two SSR markers were used to construct the genetic linkage map. The map spanned a total length of 2555.78 cM with an average of 5.08 cM distance between the markers.…”

“…A mapping population consisting of 169 F 4:7 RILs developed through single seed descent method from a cross between cultivars ‘Williams 82’ and ‘PI 366121’ was utilized in the present study (Lee et al. ). The generation of RILs for the years 2013, 2014 and 2015 was F 4:7, F 4:8 and F 4:9, respectively.…”

“…) using the GoldenGate assay (Lee et al. ). This genotype information was utilized for linkage analysis.…”

“…, Lee et al. ), which can be utilized to map chromosomal loci controlling important traits (Mohan et al. , Xu et al.…”

Identificação de QTLs em soja associados à resistência aos percevejos e a caracteres agronômicos utilizando a abordagem de mapeamento multivariado

“…Hwang (2010) Chapman et al 2003;Chung et al 2003;Nichols et al 2006;Bachlava et al 2009;Kuroda et al 2013;Hu et al 2014;Qi et al 2014a). Os QTLs identificados no GL I, por Hu et al 2014 (Mansur et al 1993;Lark et al 1994;Lark et al 1995;Mansur et al 1996;Orf et al 1999;Specht et al 2001;Wang et al 2004;Zhang et al 2004;Hwang 2010 (Lee et al 1996;Kim et al 2012), no GL C2 (Specht et al 2001;Wang et al 2004;Zhang et al 2004;Reinprecht et al 2006;Gai et al 2007;Palomeque et al 2009b;Liu et al 2011;Rossi et al 2013;Lee et al 2015), no GL D2 (Kim et al 2012), no GL I Chapman et al 2003), no GL J (Sun et al 2006) e no GL M (Specht et al 2001;Wang et al 2004;Zhang et al 2004;Pathan et al 2013;Yao et al 2015 (Mansur et al 1996;Zhang et al 2004;Sun et al 2006;Josie et al 2007;Palomeque et al 2009b;Hwang 2010;…”