Detection of Occult Micrometastases in Non-Small Cell Lung Carcinoma by Reverse Transcriptase-Polymerase Chain Reaction

Salerno, Christopher T.; Frizelle, Sandra; Niehans, Gloria A.; Ho, Sam; Jakkula, Molathi; Kratzke, Robert A.; Maddaus, Michael A.

doi:10.1378/chest.113.6.1526

Cited by 69 publications

(49 citation statements)

References 38 publications

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“…In 1 study, in which MUC1 was used as a marker, LN micrometastases were found in 6 of 10 patients. 44 In another study, RT-PCR for carcinoembryonic antigen was positive in 25% of LNs from patients who had histologic Stage I NSCLC. No mortality or recurrence data were provided by those investigators.…”

Section: Discussionmentioning

confidence: 94%

Sentinel lymph node mapping and molecular staging in nonsmall cell lung carcinoma

Pulte

Crawford

et al. 2005

Cancer

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An overview on all hydrogen‐bonded helix types, which can be expected in the novel class of β/δ‐hybrid peptides, is provided on the basis of ab initio molecular orbital theory. The results show a considerable potential of different folding patterns, which makes this novel class of peptide foldamers interesting for peptide and protein design. Thus, this study may stimulate the synthesis of such hybrid peptides. The large number of helix types found for β/δ‐hybrid peptides together with the many helices experimentally found and theoretically predicted in the other classes of peptide and hybrid peptide foldamers provide a good basis to establish general correspondence rules for their mutual exchange in peptide sequences. This is done by introduction of the concept of pattern and shape correspondence. © 2010 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 94: 279–291, 2010. This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com

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Section: Discussionmentioning

confidence: 94%

Sentinel lymph node mapping and molecular staging in nonsmall cell lung carcinoma

Pulte

Crawford

et al. 2005

Cancer

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“…Nevertheless, HLAIC expression in LNs was significantly higher than in tumor samples, which can be explained by an increased level of expression of HLAIC in lymphocytes, especially in patients with lung cancer. 32 We tested MUC1 mRNA detection by RT-PCR, as it has been reported by other authors to be sensitive for the detection of occult micrometastases in mediastinal LNs from resected NSCLCs: MUC1 mRNA was detected by nested RT-PCR in 37.5% 16 and 9.5% 33 of pN0 mediastinal LNs. However, when evaluating this marker on tumor samples in our study, 10 tumors (23%) were negative for MUC1.…”

Section: Discussionmentioning

confidence: 99%

“…Methods based on reverse transcription-polymerase chain reaction (RT-PCR) allow analysis of the entire LN and have been demonstrated to be more sensitive than immunohistochemistry. [15][16][17] However, previous studies using molecular markers in lung cancers have been suspected to have a low specificity 18,19 or be restricted to tumors with mutant DNA. 17 Moreover, as NSCLCs present heterogeneous gene expression, the use of a single marker has been suspected to lead to false negative results.…”

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confidence: 99%

“…21 The 3 markers were mRNAs from cytokeratin 19 (CK19), cytokeratin 7 (CK7) and mucin type 1 (MUC1), as CK19 22,23 and CK7 23 have been demonstrated to be components of the cancer cell cytoskeleton in NSCLC and MUC1 is a cell surface glycoprotein expressed in lung tissue. Although cross-reaction with noncarcinomatous cells has been previously reported, 24,25 MUC1 has been shown to be expressed in most NSCLCs (approximately 80-100%) 16 and is used for the detection of occult cancer cells. The aim of this study was therefore to test the accuracy of each of these markers as well as their combined use.…”

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confidence: 99%

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Real‐time RT‐PCR detection of CK19, CK7 and MUC1 mRNA for diagnosis of lymph node micrometastases in non small cell lung carcinoma

Saintigny

Coulon

Kambouchner

et al. 2005

Intl Journal of Cancer

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Metastatic lymph nodes (LNs) are the major prognostic factor in resected non small cell lung carcinoma (NSCLC). However, almost 50% of pN0 patients relapse, suggesting metastatic cells undetected by current staging procedures. A combination of markers [cytokeratins 19 and 7 (CK19, CK7) and mucin type 1 (MUC1) mRNAs] was therefore evaluated by real-time RT-PCR in order to detect occult cancer cells. Forty-three NSCLC tumor samples, 4 micrometastatic, 6 metastatic and 84 histologically negative mediastinal LNs from 19 patients with NSCLC were evaluated as well as blood mononuclear cells from 29 healthy volunteers and 17 benign LNs. When tested on cell lines, RT-PCR was particularly efficient for evaluation of CK19, CK7 and MUC1 mRNA expression. All tumor samples were positive for at least 1 marker and 74% of samples were positive for all 3 markers. CK7 and CK19 mRNA were not detected in benign LN and blood cells from healthy donors in contrast with MUC1 mRNA. Only CK7 and CK19 mRNA were therefore used for evaluation of mediastinal LNs: the 6 histologically metastatic and the 4 micrometastatic LNs were positive for at least one marker. Among the 84 histologically negative LNs, 6 (7%) were positive for at least one marker, potentially changing the stage of 2 out of 19 patients. In conclusion, in our feasibility study, parallel molecular detection of CK19 and CK7 mRNA can be considered a specific diagnostic tool for the assessment of microscopic lymphatic spread. Its prognostic impact remains to be evaluated in a prospective study. ' 2005 Wiley-Liss, Inc.

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“…More recently, it has been suggested that the RT-PCR method is more rapid and sensitive. 4,21,29,30 PCR methods have been reported for the detection of CEA, 21 CK19, 31 preprogrowth hormone receptor 20 and MUC1 32 from blood samples of lung cancer patients.…”

mentioning

confidence: 99%

Application of differential display to identify genes for lung cancer detection in peripheral blood

Matsunaga

Hangai

Aso

et al. 2002

Intl Journal of Cancer

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Detection of Occult Micrometastases in Non-Small Cell Lung Carcinoma by Reverse Transcriptase-Polymerase Chain Reaction

Cited by 69 publications

References 38 publications

Sentinel lymph node mapping and molecular staging in nonsmall cell lung carcinoma

Sentinel lymph node mapping and molecular staging in nonsmall cell lung carcinoma

Real‐time RT‐PCR detection of CK19, CK7 and MUC1 mRNA for diagnosis of lymph node micrometastases in non small cell lung carcinoma

Application of differential display to identify genes for lung cancer detection in peripheral blood

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