Detection of Orf Virus from an Outbreak in Goats and Its Genetic Relation with Other Parapoxviruses

Mondal, Bimalendu; Bera, Asit Kumar; Hosamani, M.; Tembhurne, P.A.; Bandyopadhyay, S.

doi:10.1007/s11259-006-3270-z

Cited by 45 publications

(35 citation statements)

References 35 publications

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“…Higher morbidity from orf (61%) has been reported in the Central Himalayas (29.48° N, 79.65° E) [18]. However, the prevalence detected in the current study was higher than that in England, which has been calculated as 1.88% for ewes and 19.51% for lambs [19].…”

Section: Discussioncontrasting

confidence: 78%

Orf in Goats in China: Prevalence and Risk Factors

Gao¹,

Zhao²,

JianPeng³

et al. 2016

JAST-A

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Abstract:Orf is an important viral disease that affects goats and sheep and results in large economic losses. The aim of this study was to investigate the prevalence of orf and identify the potential risk factors of this disease in the main breeding areas of China. Among 1,241 blood samples collected from goats without clinical signs of orf, 433 samples (34.89%) were positive for orf virus infection, which was detected by polymerase chain reaction (PCR) targeting a partial B2L sequence of the viral genome. Moreover, a total of 874 buccal swab samples were collected, of which 64 samples (7.32%) were positive for the orf virus on the basis of PCR detection. According to logistic regression, all of the variables, including age, breed, location and farm management, had significant impacts on the prevalence of orf. Lambs under intensive management in Yunnan province were more susceptible to orf virus infection than animals in other groups. Anglo-Nubian goats were at more risk of orf positivity than other breeds, whereas Saanen dairy goats were at significantly less risk. In summary, as the first epidemiological study of orf in China, this investigation suggested that orf is a neglected disease that requires more attention in the future.

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Section: Discussioncontrasting

confidence: 78%

Orf in Goats in China: Prevalence and Risk Factors

Gao¹,

Zhao²,

JianPeng³

et al. 2016

JAST-A

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“…The genome encodes for 130 proteins classified as early, intermediate early and late proteins [7,20]. The virus has a worldwide distribution [22] with an incidence of up to 90 % [6]. Both young and adult animals are susceptible to the virus infection.…”

Section: Introductionmentioning

confidence: 99%

Isolation and phylogenetic analysis of caprine Orf virus in Malaysia

et al. 2015

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Orf virus is a DNA virus that causes contiguous ecthyma in goat and sheep. Infection of animals with this virus cause high mortality in young animals resulting in huge economic losses. In this study, we investigated an outbreak of Orf in a goat farm in Malaysia. Samples were collected from infected animals and viral isolation was done using both LT and MDCK cell lines. Molecular detection was done by conventional PCR for specific primers; B2L and F1L genes and phylogenetic analysis was done on the sequence data obtained. Cytopathic effects (CPE) were observed in both cell lines after 3 days of inoculation and were 50 % by the sixth day. PCR showed positive bands for both B2L and F1L genes and phylogenetic analysis showed that the Malaysian strain had close homology to the Chinese and Indian Orf virus isolates. This study gives more insight into the existing Orf viral strains in Malaysia and their relationship with other strains globally.

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“…Orf is a non-systemic eruptive skin disease that occurs worldwide in sheep and goats (Mondal et al, 2006). Orf virus (ORFV) is a DNA virus belongs to the genus Parapoxvirus, sub family Chordopoxvirinae of Poxviridae family.…”

Section: Introductionmentioning

confidence: 99%

Effect of Stabilization and Reconstitution on the Stability of a Novel Strain of Live Attenuated Orf Vaccine (ORFV MUK59/05)

Bora¹,

Bhanuprakash²,

Venkatesan³

et al. 2015

Asian J. of Animal and Veterinary Advances

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The study describes the effect of stabilization on the stability of an indigenous live attenuated orf vaccine with stabilizers like LS, LHT and TAA after lyophilization and also reconstitution with NSS, PBS, distilled water and 1 M MgSO 4 exposed at different temperatures under established lyophilization conditions. The stability of the vaccine was assessed both in freeze-dried and reconstituted forms at different temperatures. The results indicated that the orf vaccine lyophilized with LS stabilizer at 25 and 45°C and LHT at 37°C found superior over others in stabilizing the keeping quality of the vaccine. Intrinsic thermo-stability studies revealed the rapid deterioration of the vaccine, when compared to the vaccine stabilized with the either of the stabilizers. Among the diluents used for reconstitution of the vaccine, quality in terms of infectivity titers of the virus was well preserved in vaccine diluted with 0.85% NSS compared to other diluents like PBS, DW and 1 M MgSO 4 . The 1 M MgSO 4 found unsuitable for diluting the orf vaccine. The study suggests that the LS at 25 and 45°C and LHT at 37°C are the choice of stabilizers and 0.85% NSS is the choice of diluent for orf vaccine at all temperatures contemplated.

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Detection of Orf Virus from an Outbreak in Goats and Its Genetic Relation with Other Parapoxviruses

Abstract: Detection of orf virus from an outbreak in goats and its genetic relation with other parapoxviruses. Veterinary Research Communications, 30(5), 531-539

Cited by 45 publications

References 35 publications

Orf in Goats in China: Prevalence and Risk Factors

Orf in Goats in China: Prevalence and Risk Factors

Isolation and phylogenetic analysis of caprine Orf virus in Malaysia

Effect of Stabilization and Reconstitution on the Stability of a Novel Strain of Live Attenuated Orf Vaccine (ORFV MUK59/05)

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