Detection of OXA‐type carbapenemases and integrons among carbapenem‐resistant <i>Acinetobactor baumannii</i> in a Teaching Hospital in China

Hu, Qiaojuan; Hu, Zhidong; Li, Jing; Tian, Bin; Hai-ru, XU; Li, Jin

doi:10.1002/jobm.201000402

Cited by 22 publications

(16 citation statements)

References 32 publications

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“…PCR detection of the extended spectrum β-lactamase genes bla TEM , bla CTX-M , bla PER , bla SHV , bla DHA , and bla CMY 22232425 in 44551 showed negative results. The observation of bla OXA-23 as the most prevalent carbapenemase gene in A. baumannii is consistent with previous findings26.…”

Section: Resultssupporting

confidence: 93%

“Roar” of blaNDM-1 and “silence” of blaOXA-58 co-exist in Acinetobacter pittii

Zhou

Chen

Meng

et al. 2015

Sci Rep

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Acinetobacter pittii 44551 was recovered from a patient with gout combined with tuberculosis and was found to harbor the carbapenemase genes blaNDM-1 and blaOXA-58 on two different plasmids pNDM-44551 and pOXA58-44551, respectively. pNDM-44551 displayed high self-transferability across multiple bacterial species, while pOXA58-44551 was likely co-transferable with pNDM-44551 into A. baumannii receipts. pNDM-44551 was a close variant of the previously characterized pNDM-BJ01, and the blaNDM-1 gene cluster was arranged sequentially as orfA, ISAba14, aphA6, ISAba125, blaNDM-1, bleMBL, ΔtrpF, dsbC, tnpR, and zeta. pOXA58-44551 was a repAci9-containing plasmid, and blaOXA-58 was embedded in a 372F-ISAba3-like-blaOXA-58-ISAba3 structure. The mobile genetic platforms of blaNDM-1 and blaOXA-58 herein showed some differences from their previously characterized variants. The production of NDM-1 in strain 44551 contributed the majority to its high resistance to carbapenems, while the blaOXA-58 stayed silent most likely due to the lack of an upstream promoter to drive its transcription. Increased surveillance of Acinetobacter co-harboring blaNDM-1 (active) and blaOXA-58 (either active or silent) is urgently needed.

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Section: Resultssupporting

confidence: 93%

“Roar” of blaNDM-1 and “silence” of blaOXA-58 co-exist in Acinetobacter pittii

Zhou

Chen

Meng

et al. 2015

Sci Rep

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“…Among A. baumannii strains, metallo-β-lactamases (MBLs) producers are widespread worldwide and plasmid mediated MBLs are responsible for carbapenem resistance (21). Recently, frequency of MBLs producing A. baumannii strains are increasing and detection of MBLs producers are important in clinical settings (22). In addition, for epidemiologic surveys, multiplex-PCR technique may be very helpful and reduce the cost of various PCR reactions.…”

Section: Discussionmentioning

confidence: 99%

Development of multiplex PCR for rapid detection of metallo-β-lactamase genes in clinical isolates of Acinetobacter baumannii

Ranjbar¹,

Zayeri²,

Mirzaie³

2020

IJM

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Background and Objectives: Acinetobacter baumannii has been known as a major pathogen causing nosocomial infec- tions. The aim of this study was to develop multiplex PCR for rapid and simultaneous detection of metallo-β-lactamase (MBL) genes in clinical isolates of A. baumannii. Materials and Methods: In this study, we used three sets of primers to amplify the MBL genes including bla , bla and bla OXA-48 . The multiplex PCR assay was optimized for rapid and simultaneous detection of MBL genes in A. bau- OXA-23 NDM mannii strains recovered from clinical samples. Results: A. baumannii strains recovered from clinical samples were subjected to the study. The multiplex PCR produced 3 OXA-48 OXA-23 bands of 501 bp for bla , 744 bp for bla observed in multiplex PCR. OXA-48 and 623 bp for bla NDM genes. In addition to, no any cross-reactivity was Conclusion: Based on obtained data, the multiplex PCR had a good specificity without any cross reactivity and it appears that the multiplex PCR is reliable assay for simultaneous detection of MBL genes in A. baumannii strains.

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“…A small number of species in these genera are human pathogens, such as certain Corynebacterium species that can cause acute disease, most notably diphtheria (Costa, Michel, Rappuoli, & Murphy, 1981). Additionally, some Acinetobacter species frequently cause bacteraemia, urinary tract infections, meningitis, infective endocarditis and infections of wounds and burns (Siegman-Igra, Bar-Yosef, Gorea, & Avram, 1993;Hu et al, 2011).…”

Section: Profile Of Bacteria Distribution In Each Samplementioning

confidence: 99%

Tick saliva microbiomes isolated from engorged and partially fed adults of Haemaphysalis flava tick females

Cheng

2017

J Applied Entomology

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The tick Haemaphysalis flava is one of the most significant blood-feeding arthropod parasites and is a vector for numerous human and animal pathogens. However, a comprehensive investigation of the microbial communities found in the saliva of this tick species is lacking. This study used 16S rRNA Illumina sequencing to characterize the compositions of microbiomes present in saliva and whole tick samples isolated from engorged and partially fed adult H. flava females. This revealed that the bacterial diversity present in tick saliva increased after a prolonged blood meal, and that the species diversity found in saliva was significantly higher than that of whole ticks. Three bacteria phyla, in particular, made up more than 80% of the microbial community across all samples-Proteobacteria, Firmicutes and Actinobacteria. Furthermore, some of the genera identified in this study had not previously been reported in ticks before, such as Facklamia, Vagococcus, Ruminococcus, Lachnospira, Bradyrhizobium, Peptostreptococcus, Jeotgalicoccus, Roseburia, Brachybacterium, Sporosarcina, u114, Megamonas and Dechloromonas. Finally, we found that many of the isolated bacteria were opportunistic pathogens, indicating a potential risk to humans and livestock exposed to H. flava.These results will contribute to fully understanding the transmission of tick-borne pathogens. K E Y W O R D Sbacteria, Haemaphysalis flava, microbiome, saliva | INTRODUCTIONHaemaphysalis flava (Acari: Ixodidae) is a prevalent hard tick species found in many countries that feeds on a wide variety of hosts, including hares, dogs, cows, horses, wild boar, deer, bears, pigs, cattle, sheep, goats, hedgehogs, pandas, cats, squirrels and golden pheasants (He, Tang, & Cheng, 2016). As a vector for numerous pathogens, including encephalitis virus (Ko et al., 2010), Borrelia burgdorferi (Moon et al., 2013), Rickettsia (Someya, Ito, Maeda, & Ikenaga, 2015), Francisella tularensis (Kim et al., 2005), Bartonella (Ozawa et al., 1982), Anaplasma and Ehrlichia (Rar et al., 2010), H. flava has emerged as a significant threat to public health.Tick saliva plays a pivotal role in pathogen transmission (Nuttall & Labuda, 2004) and studying the microbes that inhabit it can contribute to both conventional disease discovery (in which a pathogen is discovered in a host and then identified in the tick population) and reverse discovery (in which a potential pathogen is characterized in ticks and then is subsequently shown to cause disease) (Tijsse-Klasen, Koopmans, & Sprong, 2014). However, information on the microbial populations present in tick saliva remains scarce. To date, the saliva microbiomes of Amblyomma maculatum (Budachetri et al., 2014) and Rhipicephalus microplus (Xiang, Poźniak, & Cheng, 2017) are the only two that have been surveyed. The microflora of saliva isolated from H. flava has been partially analysed using PCR-denaturing gradient gel electrophoresis (PCR-DGGE), but this system is unable to reveal in detail the microbial communities of saliva (He et al...

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Detection of OXA‐type carbapenemases and integrons among carbapenem‐resistant Acinetobactor baumannii in a Teaching Hospital in China

Cited by 22 publications

References 32 publications

“Roar” of blaNDM-1 and “silence” of blaOXA-58 co-exist in Acinetobacter pittii

“Roar” of blaNDM-1 and “silence” of blaOXA-58 co-exist in Acinetobacter pittii

Development of multiplex PCR for rapid detection of metallo-β-lactamase genes in clinical isolates of Acinetobacter baumannii

Tick saliva microbiomes isolated from engorged and partially fed adults of Haemaphysalis flava tick females

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