Detection of oxidative stress in seminal plasma and fractionated sperm from subfertile male patients

Nakamura, Hitomi; Kimura, Tadashi; Nakajima, Atsuko; Shimoya, Koichiro; Takemura, Masahiko; Hashimoto, Kazumasa; Isaka, Shigeyuki; Azuma, Chihiro; Koyama, Masayasu; Murata, Yuji

doi:10.1016/s0301-2115(02)00194-x

Cited by 43 publications

(32 citation statements)

References 16 publications

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“…Our results are agreed with some previous studies that show increasing of lipid peroxidation by measuring MDA in sperm and seminal plasma in males with asthenozoospermia, asthenoteratozoospermia and oligoasthenoteratozoospemia (13,15). Similar to MDA (11, 16), 8-Isoprostane also showed an indirect correlation with sperm motility.…”

Section: Discussionsupporting

confidence: 93%

“…The toxicity of generated fatty acid peroxides are important causes of sperm malfunction (3)(4)(5)(6)(7)(8)(9). Available data on the impact of oxidative stress on sperm are based on the measurement of seminal plasma and sperm malondialdehyde (MDA) by the thiobarbituric acid-reacting substance (TBARS) assay (10)(11)(12)(13)(14)(15)(16)(17).…”

Section: Introductionmentioning

confidence: 99%

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Seminal plasma levels of free 8-isoprostane and its relationship with sperm quality parameters

Khosrowbeygi

Zarghami

2008

Indian J Clin Biochem

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There is growing evidence that damage to spermatozoa by reactive oxygen species play a key role in male infertility. The aim of this study was to assess seminal plasma free 8-Isoprostane levels in men with asthenozoospermia, asthenoteratozoospermia and oligoasthenoteratozoospermia compared to normozoospermic males and its correlation with seminal parameters. The case group consisted of men with asthenozoospermia (n=15), asthenoteratozoospermia (n=16) and oligoasthenoteratozoospermia (n=15). The control group consisted of 16 males with normozoospermia. After Purification of Free 8-Isoprostane by affinity column, its concentration was measured by enzyme immunoassay method. Free 8-Isoprostane evaluation showed significantly greater values in the total case group (n=46) versus control group (18.23±3.56 vs 2.6±0.38 ng/ml). In each case group free 8-Isoprostane also showed a significant increasing compared to normozoospermic males. Free 8-Isoprostane showed an inversely significant correlation with sperm motility and sperm morphology. Lipid peroxidation could have significant role in etiology of sperm abnormalities. Measurement of 8-Isoprostane can be used as a specific biomarker for assessing lipid peoxidation in sperm.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Seminal plasma levels of free 8-isoprostane and its relationship with sperm quality parameters

Khosrowbeygi

Zarghami

2008

Indian J Clin Biochem

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“…Increased ROS production is inversely correlated with sperm function [76]. Several studies have shown an association between sperm motility and leukocyte Mahmoud et al [7] T/LH ↓ Young: 5.5 nmol/U Older: 2.5 nmol/U contamination in ejaculate, ROS production, lipid peroxidation, and sperm oxidative DNA damage [72,77,78]. Some studies have indicated higher levels of dsDNA breaks and higher DNA fragmentation index (DFI) in older men [79][80][81][82] (Table 4).…”

Section: Free Radical Theory Of Aging and Apoptosismentioning

confidence: 99%

Effects of aging on the male reproductive system

Güneş

Hekim

Arslan

et al. 2016

J Assist Reprod Genet

197

118

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The study aims to discuss the effects of aging on the male reproductive system. A systematic review was performed using PubMed from 1980 to 2014. Aging is a natural process comprising of irreversible changes due to a myriad of endogenous and environmental factors at the level of all organs and systems. In modern life, as more couples choose to postpone having a child due to various socioeconomic reasons, research for understanding the effects of aging on the reproductive system has gained an increased importance. Paternal aging also causes genetic and epigenetic changes in spermatozoa, which impair male reproductive functions through their adverse effects on sperm quality and count as, well as, on sexual organs and the hypothalamic-pituitarygonadal axis. Hormone production, spermatogenesis, and testes undergo changes as a man ages. These small changes lead to decrease in both the quality and quantity of spermatozoa. The offspring of older fathers show high prevalence of genetic abnormalities, childhood cancers, and several neuropsychiatric disorders. In addition, the latest advances in assisted reproductive techniques give older men a chance to have a child even with poor semen parameters. Further studies should investigate the onset of gonadal senesce and its effects on aging men.

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“…This can be measured in sperm or seminal plasma by HPLC [125], enzyme-linked immunosorbent assay [126] or directly within sperm using immunofluorescence (Kao et al [124]). De Iuliis et al correlated 8OHdG levels with the degree of sperm DNA damage.…”

Section: Measurement Of 8-ohdgmentioning

confidence: 99%

Supraphysiological Free Radical Levels and their Pathogenesis in Male Infertility

Dinesh¹

2012

Reprod Sys Sexual Disorders

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Oxidative stress is an important aetiological factor which leads to sperm DNA damage and infertility. It damages all biomolecules and both mitochondrial and nuclear DNA and adversely affects sperm membrane fluidity and motility. This acts like a biological safeguard however use of such sperm for ART/ICSI can lead to pre and post implantation losses, major and minor congenital malformations and even childhood cancer. Thus it is important to know the causes of oxidative stress and how the levels of free radicals be maintained at physiological levels when they are beneficial for normal function. It is also important to develop techniques to identity cases with high free radical levels and also adopt certain life style measures which can minimise oxidative stress and improve male reproductive health.

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Detection of oxidative stress in seminal plasma and fractionated sperm from subfertile male patients

Cited by 43 publications

References 16 publications

Seminal plasma levels of free 8-isoprostane and its relationship with sperm quality parameters

Seminal plasma levels of free 8-isoprostane and its relationship with sperm quality parameters

Effects of aging on the male reproductive system

Supraphysiological Free Radical Levels and their Pathogenesis in Male Infertility

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