Detection of parathyroid hormone using an electrochemical impedance biosensor based on PAMAM dendrimers

Abstract

This paper presents a novel hormone-based impedimetric biosensor to determine parathyroid hormone (PTH) level in serum for diagnosis and monitoring treatment of hyperparathyroidism, hypoparathyroidism and thyroid cancer. The interaction between PTH and the biosensor was investigated by an electrochemical method. The biosensor was based on the gold electrode modified by 12-mercapto dodecanoic (12MDDA). Antiparathyroid hormone (anti-PTH) was covalently immobilized on to poly amidoamine dendrimer (PAMAM) which was bound to a 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide/N-hydroxysuccinimide (EDC/NHS) couple, self-assembled monolayer structure from one of the other NH2 sites. The immobilization of anti-PTH was monitored by electrochemical impedance spectroscopy, cyclic voltammetry and scanning electron microscope techniques. After the optimization studies of immobilization materials such as 12MDDA, EDC-NHS, PAMAM, and glutaraldehyde, the performance of the biosensor was investigated in terms of linearity, sensitivity, repeatability, and reproducibility. PTH was detected within a linear range of 10-60 fg/mL. Finally the described biosensor was used to monitor PTH levels in artificial serum samples.

Voltammetric Determination of Isoniazid Drug in Various Matrix by Using CuOx Decorated MW‐CNT Modified Glassy Carbon Electrode

“…Artificial human serum was prepared as described Özcan et al. by using bovine serum albumin (BSA) instead of human serum albumin and contained 5 mM CaCl 2 ,4.5 mM KCl, 1.6 mM MgCl 2 , 4.7 mM d(+) glucose, 0.1 % bovine serum albumin, 2.5 mM urea, and 145 mM NaCl .…”

Point-of-care diagnostics for niche applications

“…In an attempt to develop improved PTH tests for the point-of-care in the operating theater, there have been some recent efforts to develop new diagnostic devices (Dittmer et al, 2008, Özcan and Sezgintürk, 2015, Sayikli ŞimŞek et al, 2015). Perhaps most notably, Philips has developed an intraoperative PTH assay based on their handheld magnotech technology (Bruls et al, 2009, Jarrige et al, 2011).…”

Minimization of Interferences in Flow Injection Amperometric Glucose Biosensor Based on Oxidation of Enzymatically‐produced H2O2

“…To test the applicability of the proposed biosensor, glucose levels in an artificial serum sample and two real samples (dextrose serum and glucose tolerance test drink solutions) were determined using the standard addition method in the FIA system. The artificial serum sample was prepared according to the literature by including 4.77 mM glucose, 0.2 mM AA, 0.2 mM DA and 1.0 mM UA. Before injection of these samples, the artificial serum (4.77 mM glucose), dextrose serum (decelerated glucose concentration: 252.5 mM) and glucose tolerance test drink solutions (decelerated glucose concentration: 1514 mM) samples were diluted 10, 100 and 2000 times with the carrier stream (pH 6.0 BRBS containing 1.0 M KCl), respectively.…”