Detection of pathogenic entamoeba histolytica DNA in liver abscess fluid by polymerase chain reaction

Tachibana, Hiroshi; Kobayashi, Seiki; Okuzawa, Eiichi; Masuda, Gohta

doi:10.1016/0020-7519(92)90042-j

Cited by 36 publications

(22 citation statements)

References 10 publications

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“…A recent study confirms that in the majority of successfully treated ALA patients the abscess completely resolves; however, in 7.1% of patients residual lesions are detected, with the unique sonographic appearance of round or oval hypo-or isoechoic areas surrounded by the hyperchoic wall (21). The successful use of PCR methods in detection of E. histolytica DNA in patients with ALA has shown high sensitivity (100%) (52,182,214,215). VOL.…”

Section: Extraintestinal Amebiasismentioning

confidence: 85%

“…PCR for the detection of E. histolytica DNA from liver abscess samples was first employed using the gene encoding the 30-kDa antigen, and 100% sensitivity was reported (182). In another study, PCR performed on liver samples demonstrated only 33% sensitivity for the presence of E. histolytica using primers specific for 18S rDNA of E. histolytica, whereas the second pair, specific for the 30-kDa antigen gene (182), showed a sensitivity of 100% (215).…”

Section: Conventional Pcrmentioning

confidence: 99%

“…In another study, PCR performed on liver samples demonstrated only 33% sensitivity for the presence of E. histolytica using primers specific for 18S rDNA of E. histolytica, whereas the second pair, specific for the 30-kDa antigen gene (182), showed a sensitivity of 100% (215). Direct amplification for detection of E. histolytica DNA (without the extraction of DNA) from ALA pus was reported using 10 different previously published primer pairs (used for amplification of E. his- tolytica from liver and stool samples) (214).…”

Section: Conventional Pcrmentioning

confidence: 99%

“…Direct amplification for detection of E. histolytica DNA (without the extraction of DNA) from ALA pus was reported using 10 different previously published primer pairs (used for amplification of E. his- tolytica from liver and stool samples) (214). Of the 10 different primer pairs tested, two pairs, i.e., P1-P2, targeting extrachromosomal circular DNA of E. histolytica (3), and P11-P12, targeting the 30-kDa antigen gene (182), gave 100% sensitivity. Another PCR assay (hemo-PCR), based on the novel hemolysin gene HLY6 of E. histolytica, was analyzed for the liver abscess samples.…”

Section: Conventional Pcrmentioning

confidence: 99%

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Laboratory Diagnostic Techniques for Entamoeba Species

et al. 2007

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SUMMARY The genus Entamoeba contains many species, six of which (Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, Entamoeba polecki, Entamoeba coli, and Entamoeba hartmanni) reside in the human intestinal lumen. Entamoeba histolytica is the causative agent of amebiasis and is considered a leading parasitic cause of death worldwide in humans. Although recent studies highlight the recovery of E. dispar and E. moshkovskii from patients with gastrointestinal symptoms, there is still no convincing evidence of a causal link between the presence of these two species and the symptoms of the host. New approaches to the identification of E. histolytica are based on detection of E. histolytica-specific antigen and DNA in stool and other clinical samples. Several molecular diagnostic tests, including conventional and real-time PCR, have been developed for the detection and differentiation of E. histolytica, E. dispar, and E. moshkovskii in clinical samples. The purpose of this review is to discuss different methods that exist for the identification of E. histolytica, E. dispar, and E. moshkovskii which are available to the clinical diagnostic laboratory. To address the need for a specific diagnostic test for amebiasis, a substantial amount of work has been carried out over the last decade in different parts of the world. The molecular diagnostic tests are increasingly being used for both clinical and research purposes. In order to minimize undue treatment of individuals infected with other species of Entamoeba such as E. dispar and E. moshkovskii, efforts have been made for specific diagnosis of E. histolytica infection and not to treat based simply on the microscopic examination of Entamoeba species in the stool. The incorporation of many new technologies into the diagnostic laboratory will lead to a better understanding of the public health problem and measures to control the disease.

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Section: Extraintestinal Amebiasismentioning

confidence: 85%

Section: Conventional Pcrmentioning

confidence: 99%

Section: Conventional Pcrmentioning

confidence: 99%

Section: Conventional Pcrmentioning

confidence: 99%

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Laboratory Diagnostic Techniques for Entamoeba Species

et al. 2007

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“…Tachibana et al (1991) utilizaron la PCR para diferenciar E. histolytica patógena y no patóge-na 36 y usaron la PCR (1992), en el líquido del AHA, para confirmar el diagnóstico 37 . Acuña-Soto et al (1993) aplicaron PCR en muestras de materia fecal para diferenciar E. histolytica patógena de no patógena en una población rural de México 38 .…”

Section: This Article Presents a History Of Entamoeba Histolytica Spaunclassified

Historia del protozoo Entamoeba histolytica

2008

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Clinical Diagnostic Applications of the Polymerase Chain Reaction

Rettinger

Hafenrichter²,

Flye³

1993

Arch Surg

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In this review, we describe the power and sensitivity of the polymerase chain reaction and indicate areas of clinical medicine in which it is currently being applied. As this technology and automation improves and is simplified, its realm of application will expand, but its major impact will continue to be its facilitation of early, specific diagnoses in infectious disease, genetics, cancer, and transplantation. Polymerase chain reaction is currently most useful in the care of opportunistic infections in immunosuppressed hosts and in making the distinction between infection and rejection in transplant recipients. As the technique becomes more widely available and less costly, its application should minimize the need for broad-spectrum antibiotic therapy of infections, lower costs, and perhaps even shorten hospitalization. These potential beneficial effects of polymerase chain reaction will be particularly relevant in the current cost-conscious health-care environment.

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Detection of pathogenic entamoeba histolytica DNA in liver abscess fluid by polymerase chain reaction

Cited by 36 publications

References 10 publications

Laboratory Diagnostic Techniques for Entamoeba Species

Laboratory Diagnostic Techniques for Entamoeba Species

Historia del protozoo Entamoeba histolytica

Clinical Diagnostic Applications of the Polymerase Chain Reaction

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