Detection of Pathogenic Microorganisms by Microfluidics Based Analytical Methods

Zhang, Dongxue; Bi, Hongyan; Liu, Baohong; Qiao, Liang

doi:10.1021/acs.analchem.8b00399

Cited by 127 publications

(81 citation statements)

References 73 publications

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“…The epidemic Covid-19 that broke up at the end of 2019 is a rather convincing example of how fast these diseases can spread by aerosol. Therefore, rapidly and effectively detecting them is very important and crucial for disease control and human health protection ( Zhang, Bi, Liu, & Qiao, 2018 ). To monitor these pathogenic airborne microorganisms ( Hong, Kang, Lee, Kim, & Jung, 2015 ), the first key step is to sample bioaerosols.…”

Section: Introductionmentioning

confidence: 99%

Inertial migration of aerosol particles in three-dimensional microfluidic channels

2021

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Section: Introductionmentioning

confidence: 99%

Inertial migration of aerosol particles in three-dimensional microfluidic channels

2021

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“…Culture-based assays continue to be the gold standard for bacteria identification [3]. However, this methodology is time-consuming taking several days in some cases [4]. Moreover, highly skilled labor and a specific laboratory setting is required, which can limit their use in some places where proper facilities are not available.…”

Section: Introductionmentioning

confidence: 99%

“…In the last decades, several immunological and molecular methods have been developed for pathogen diagnostics with a high level of sensitivity and accuracy. The most popular methods include immuno-based biosensors, enzyme-linked immunosorbent assay (ELISA) and molecular methods such as polymerase chain reaction (PCR), mass spectrometry (MS) and more recently, loop-mediated isothermal amplification (LAMP) and next generation sequencing (NGS) [4]. Though highly sensitive, these methods involve complex, time-consuming sample preparation steps, sophisticated laboratory equipment and highly skilled labor.…”

Section: Introductionmentioning

confidence: 99%

New solutions to capture and enrich bacteria from complex samples

Sande

Çaykara

Silva

et al. 2020

Med Microbiol Immunol

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Current solutions to diagnose bacterial infections though reliable are often time-consuming, laborious and need a specific laboratory setting. There is an unmet need for bedside accurate diagnosis of infectious diseases with a short turnaround time. Moreover, low-cost diagnostics will greatly benefit regions with poor resources. Immunoassays and molecular techniques have been used to develop highly sensitive diagnosis solutions but retaining many of the abovementioned limitations. The detection of bacteria in a biological sample can be enhanced by a previous step of capture and enrichment. This will ease the following process enabling a more sensitive detection and increasing the possibility of a conclusive identification in the downstream diagnosis. This review explores the latest developments regarding the initial steps of capture and enrichment of bacteria from complex samples with the ultimate goal of designing low cost and reliable diagnostics for bacterial infections. Some solutions use specific ligands tethered to magnetic constructs for separation under magnetic fields, microfluidic platforms and engineered nano-patterned surfaces to trap bacteria. Bulk acoustics, advection and nano-filters comprise some of the most innovative solutions for bacteria enrichment.

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“…Culture-based methods as the mainstream of phenotyping have the advantages of cost-effectiveness and simplicity, and remain the gold standard for bacterial monitoring and identification. However, it requires days for culture-based methods to provide conclusive results, which greatly hampers their applications in water quality monitoring [ 3 ]. Molecular analyses including conventional polymerase chain reaction (PCR)–based methods, immunology-based methods, etc, however, require lengthy processes of sample pretreatment (eg, concentration, cell lysis, purification), expensive equipment, and trained personnel in centralized laboratory facilities.…”

mentioning

confidence: 99%

Rapid Detection Methods for Bacterial Pathogens in Ambient Waters at the Point of Sample Collection: A Brief Review

Zhu

et al. 2020

Clinical Infectious Diseases

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The world is currently facing a serious health burden of waterborne diseases, including diarrhea, gastrointestinal diseases, and systemic illnesses. The control of these infectious diseases ultimately depends on the access to safe drinking water, properly managed sanitation, and hygiene practices. Therefore, ultrasensitive, rapid, and specific monitoring platforms for bacterial pathogens in ambient waters at the point of sample collection are urgently needed. We conducted a literature review on state-of-the-art research of rapid in-field aquatic bacteria detection methods, including cell-based methods, nucleic acid amplification detection methods, and biosensors. The detection performance, the advantages, and the disadvantages of the technologies are critically discussed. We envision that promising monitoring approaches should be automated, real-time, and target-multiplexed, thus allowing comprehensive evaluation of exposure risks attributable to waterborne pathogens and even emerging microbial contaminants such as antibiotic resistance genes, which leads to better protection of public health.

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Detection of Pathogenic Microorganisms by Microfluidics Based Analytical Methods

Cited by 127 publications

References 73 publications

Inertial migration of aerosol particles in three-dimensional microfluidic channels

Inertial migration of aerosol particles in three-dimensional microfluidic channels

New solutions to capture and enrich bacteria from complex samples

Rapid Detection Methods for Bacterial Pathogens in Ambient Waters at the Point of Sample Collection: A Brief Review

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