Detection of pathogenic Vibrio parahaemolyticus in southern Italian shellfish

Pinto, Angela Di; Ciccarese, Giuseppina; Corato, Rita De; Novello, L.; Terio, Valentina

doi:10.1016/j.foodcont.2007.10.013

Cited by 64 publications

(48 citation statements)

References 12 publications

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“…Several alternative DNA-based methods have been described for detecting V. parahaemolyticus from shellfish (Di Pinto, Ciccarese, De Corato, Novello, & Terio, 2008;Su & Liu, 2007). However, epidemiological studies require the availability of accurate culture methods for isolating V. parahaemolyticus in foods to establish effective control measures, as well as to ensure seafood safety according to Reg.…”

Section: Introductionmentioning

confidence: 99%

Comparison between thiosulphate-citrate-bile salt sucrose (TCBS) agar and CHROMagar Vibrio for isolating Vibrio parahaemolyticus

et al. 2011

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Section: Introductionmentioning

confidence: 99%

Comparison between thiosulphate-citrate-bile salt sucrose (TCBS) agar and CHROMagar Vibrio for isolating Vibrio parahaemolyticus

et al. 2011

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“…Strains that possess tdh, trh, and TTSS2-encoding genes are generally pathogenic and responsible for the vast majority of clinical cases. While such strains account for only 1 to 2% of isolates sampled from water and different marine species (4-6), they are known to have a widespread global distribution, having been reported from cases of infection in Japan, India, Thailand, Malaysia, China, Indonesia, the United Kingdom, Italy, and the East and Gulf Coasts of the United States (7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19).…”

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Genetic Relationships of Vibrio parahaemolyticus Isolates from Clinical, Human Carrier, and Environmental Sources in Thailand, Determined by Multilocus Sequence Analysis

Theethakaew

Feil

Castillo-Ramírez

et al. 2013

Appl Environ Microbiol

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e Vibrio parahaemolyticus is a seafood-borne pathogenic bacterium that is a major cause of gastroenteritis worldwide. We investigated the genetic and evolutionary relationships of 101 V. parahaemolyticus isolates originating from clinical, human carrier, and various environmental and seafood production sources in Thailand using multilocus sequence analysis. The isolates were recovered from clinical samples (n ‫؍‬ 15), healthy human carriers (n ‫؍‬ 18), various types of fresh seafood (n ‫؍‬ 18), frozen shrimp (n ‫؍‬ 16), fresh-farmed shrimp tissue (n ‫؍‬ 18), and shrimp farm water (n ‫؍‬ 16). Phylogenetic analysis revealed a high degree of genetic diversity within the V. parahaemolyticus population, although isolates recovered from clinical samples and from farmed shrimp and water samples represented distinct clusters. The tight clustering of the clinical isolates suggests that disease-causing isolates are not a random sample of the environmental reservoir, although the source of infection remains unclear. Extensive serotypic diversity occurred among isolates representing the same sequence types and recovered from the same source at the same time. These findings suggest that the O-and K-antigen-encoding loci are subject to exceptionally high rates of recombination. There was also strong evidence of interspecies horizontal gene transfer and intragenic recombination involving the recA locus in a large proportion of isolates. As the majority of the intragenic recombinational exchanges involving recA occurred among clinical and carrier isolates, it is possible that the human intestinal tract serves as a potential reservoir of donor and recipient strains that is promoting horizontal DNA transfer, driving evolutionary change, and leading to the emergence of new, potentially pathogenic strains. Vibrio parahaemolyticus is a seafood-borne pathogenic bacterium and is the leading cause of traveler's diarrhea and gastroenteritis worldwide due to the consumption of undercooked contaminated seafood, particularly shellfish. The organism possesses a number of virulence factors, including a thermostable direct hemolysin (TDH) (1), a thermostable direct hemolysinrelated hemolysin (TRH) (2), and type 3 secretion system 1 (TTSS1) and TTSS2 (3). Strains that possess tdh, trh, and TTSS2-encoding genes are generally pathogenic and responsible for the vast majority of clinical cases. While such strains account for only 1 to 2% of isolates sampled from water and different marine species (4-6), they are known to have a widespread global distribution, having been reported from cases of infection in Japan, India, Thailand, Malaysia, China, Indonesia, the United Kingdom, Italy, and the East and Gulf Coasts of the United States (7-19).The public health and commercial burdens associated with V. parahaemolyticus contamination are very high in Thailand due to the wide consumption of seafood. Although undercooked seafood has been identified as a source of V. parahaemolyticus infection (20, 21), the relative likelihood of contamination from differ...

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“…Gram-negative bacteria are the predominant amine-forming bacteria in fish, cephalopods and shellfi sh (7)(8)(9)(10)(11). Endotoxins are integral components of the outer membrane of Gram-negative bacteria, which are typically released upon bacterial lysis and during multiplication.…”

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Determination of Biogenic Amines and Endotoxin in Squid, Musky Octopus, Norway Lobster, and Mussel Stored at Room Temperature

Prester

Orct

Macan

et al. 2010

Archives of Industrial Hygiene and Toxicology

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Little research has been published on the indicators of spoilage in Mediterranean molluscan shellfi sh and crustaceans. Thus is why we studied changes in the concentrations of endotoxin and four biogenic amines (histamine, putrescine, tyramine and cadaverine) in European common squid (Loligo subulata, Lamarck, 1798), musky octopus (Eledone moschata, Lamarck, 1798), Norway lobster (Nephrops norvegicus, Linnaeus, 1758), and mussel (Mytilus galloprovincialis, Lamarck, 1819) from the Adriatic Sea stored at room temperature for 24 h. Endotoxin load in fresh squid, Norway lobster, and mussel (<1 EU mg -1 ) indicated good microbiological quality of raw samples. Biogenic amine index (as the sum of histamine, putrescine, tyramine, and cadaverine) correlated well with endotoxin load in squid (r=0.978, p<0.001) and musky octopus (r=0.874, p<0.01). A good correlation was also found between endotoxin and putrescine in Norway lobster (r=0.777, p<0.05). The highest endotoxin load was found in decomposed mussels and was associated with histamine alone. In conclusion, increase in biogenic amine levels is species-specifi c. Endotoxin analysis could be used for rapid assessment of microbiological quality of cephalopods and shellfi sh.

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Detection of pathogenic Vibrio parahaemolyticus in southern Italian shellfish

Cited by 64 publications

References 12 publications

Comparison between thiosulphate-citrate-bile salt sucrose (TCBS) agar and CHROMagar Vibrio for isolating Vibrio parahaemolyticus

Comparison between thiosulphate-citrate-bile salt sucrose (TCBS) agar and CHROMagar Vibrio for isolating Vibrio parahaemolyticus

Genetic Relationships of Vibrio parahaemolyticus Isolates from Clinical, Human Carrier, and Environmental Sources in Thailand, Determined by Multilocus Sequence Analysis

Determination of Biogenic Amines and Endotoxin in Squid, Musky Octopus, Norway Lobster, and Mussel Stored at Room Temperature

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