1997
DOI: 10.1016/s0168-1605(97)00069-x
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Detection of pathogenic Yersinia enterocolitica in milk and pork using a DIG-labelled probe targeted against the yst gene

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Cited by 18 publications
(7 citation statements)
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“…Thus, several investigations have been undertaken to develop rapid and reliable methods for detection of pathogenic Yersinia strains from clinical, food, and environmental samples. By using DNA colony hybridization assays, pathogenic Y. enterocolitica strains are detected using gene probes targeting the virulence plasmid (70, 100) or virulencerelated DNA sequences in the chromosome (37,55).…”
Section: Colony Hybridization Methodsmentioning
confidence: 99%
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“…Thus, several investigations have been undertaken to develop rapid and reliable methods for detection of pathogenic Yersinia strains from clinical, food, and environmental samples. By using DNA colony hybridization assays, pathogenic Y. enterocolitica strains are detected using gene probes targeting the virulence plasmid (70, 100) or virulencerelated DNA sequences in the chromosome (37,55).…”
Section: Colony Hybridization Methodsmentioning
confidence: 99%
“…The regulatory virF gene plays a major role in the coordinated thermal induction of virulence determinants (21), while the yadA gene encodes a multifaceted outer membrane protein, YadA, which is an essential virulence factor of Y. enterocolitica (38). Goverde et al (55) designed a colony hybridization method using probes targeting the chromosomal ail and inv genes, and Durisin et al (37) designed a method involving the yst gene. ail codes for the membrane-associated protein Ail, which promotes invasion into eukaryotic cells and confers serum resistance (16,102).…”
Section: Colony Hybridization Methodsmentioning
confidence: 99%
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“…In addition, the probes are reusable, easily stored, and stripped from the membranes. Previous researchers have used DIG-labeled probes for the detection of Staphylococcus aureus (33) and Yersinia enterocolitica in foods (16). Similarly, randomly primed DIG labeling of a probe specific for C. jejuni subsp.…”
Section: Discussionmentioning
confidence: 99%
“…; Iushchenko et al, 1982;Pavlov, 1985;Ding et al, 1986;Schiemann, 1987;De Centorbi et al, 1989;Hamama et al, 1992;Durisin et al, 1997) A wide range of animals: domestic (cat and dog), stock farm (chinchilla, mink, pig, rabbit, cow, goose, horse, sheep, and buffalo), zoo (monkey), wild (raccoon, fox, snail, frog, beaver, deer, ocelot, crab, flies, and fleas), birds (robin), shellfish (oyster), and many species of small rodents (McClure et al, 1971;Wauters et al, 1971;Alonso and Bercovier, 1975;Toma and Deidrick, 1975;Wauters and Janssens, 1976;Kapperud, 1977;Servan et al, 1979;Iushchenko et al, 1982;Gurleva et al, 1985;Kato et al, 1985;Walker and Grimes, 1985;Shayegani et al, 1986;Weber et al, 1987;Cox et al, 1990;Escudero et al, 1991;Weynants et al, 1996;Zheng and Xie, 1996;Funk et al, 1998;Letellier et al, 1999;Naktin and Beavis, 1999) Most of the strains isolated from the environment belong to the nonpathogenic biotype 1A, with the exception of pathogenic strains of biotype 1B, which are frequently isolated from water.…”
Section: Yersinia Enterocoliticamentioning
confidence: 99%