Detection of Pathogens in Water Using Micro and Nano-Technology

Zuccheri, Giampaolo; Asproulis, Nikolaos

doi:10.2166/9781780401096

Cited by 4 publications

(3 citation statements)

References 393 publications

(540 reference statements)

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“…As the technique employed a label-free sensor system commercially available, an early warning for the contamination could be feasible via real-time monitoring. 48,49 Conclusions Aspects of the innate immune system of animal cells (e.g., the RAW264.7 cell line) were used as a biosensor for the real-time monitoring of bacterial contamination so that sample testing could be carried out in an automated manner without handling. 46 Furthermore, the non-targeted detection concept may also be used to screen foods containing bacteria exceeding a certain threshold number.…”

Section: Establishment Of Experimental Biosensor Modelsmentioning

confidence: 99%

“…Such ability for the bacterial analysis may substantially simplify the food quality control process without using an additional sample pretreatment. Because the infectious stimulation is initiated by the interaction of PAMPs and pattern recognition receptors, 50 the concept could be applied in principle to the analyses of non-targeted microbial species such as bacteria, viruses, and fungi, which may contaminate, 48,49,51 for example, foods, the environment, or even human blood. For example, the laboratory pasteurization count of milk and its fermented products 47 may be readily conducted as a management practice using the same sensing technique.…”

Section: Establishment Of Experimental Biosensor Modelsmentioning

confidence: 99%

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An innate immune system-mimicking, real-time biosensing of infectious bacteria

Seo

Jeon

Kim

et al. 2015

Analyst

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An animal cell-based biosensor was investigated to monitor bacterial contamination in an unattended manner by mimicking the innate immune response. The cells (RAW 264.7 cell line) were first attached onto the solid surfaces of a 96-well microtiter plate and co-incubated in the culture medium with a sample that might contain bacterial contaminants. As Toll-like receptors were present on the cell membrane surfaces, they acted as a sentinel by binding to pathogen-associated molecular patterns (PAMPs) of any contaminant. Such biological recognition initiates signal transmission along various pathways to produce different proinflammatory mediators, one of which, tumor necrosis factor-α (TNF-α) was measured using an immunosensor. To demonstrate automated bacterium monitoring, a capture antibody specific for TNF-α was immobilized on an optical fiber sensor tip and then used to measure complex formation in a label-free sensor system (e.g., Octet Red). The sensor response time depended significantly on the degree of agitation of the culture medium, controlling the biological recognition and further autocrine/paracrine signaling by cytokines. The response, particularly under non-agitated conditions, was also influenced by the medium volume, revealing a local gradient change of the cytokine concentration and also acidity, caused by bacterial growth near the bottom surfaces. A biosensor system retaining 50 μL medium and not employing agitation could be used for the early detection of bacterial contamination. This novel biosensing model was applied to the real-time monitoring of different bacteria, Shigella sonnei, Staphylococcus aureus, and Listeria monocytogenes. They (<100 CFU mL(-1)) could be detected automatically within the working time. Such analysis was carried out without any manual handling regardless of the bacterial species, suggesting the concept of non-targeted bacterial real-time monitoring. This technique was further applied to real sample testing (e.g., with milk) to exemplify, for example, the food quality control process without using any additional sample pretreatment such as magnetic concentration.

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Section: Establishment Of Experimental Biosensor Modelsmentioning

confidence: 99%

Section: Establishment Of Experimental Biosensor Modelsmentioning

confidence: 99%

An innate immune system-mimicking, real-time biosensing of infectious bacteria

Seo

Jeon

Kim

et al. 2015

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“…One could easily imagine how serious the situation could be in the presence of dangerous pathogens resistant to treatment. Cryptosporidium for instance has already been detected in water despite the absence of these indicators, 3,5 and is routinely tested for in UK waters. The development of new approaches is thus a growing and necessary research area leading to several new national, European and international projects.…”

Section: Introductionmentioning

confidence: 99%

Angry pathogens, how to get rid of them: introducing microfluidics for waterborne pathogen separation to children

Jimenez

Bridle

2015

Lab Chip

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The purpose of this paper is to present a new approach for introducing to a non-scientific audience a major public health issue: access to safe drinking water. Access to safe drinking water is a privilege in developed countries and an urgent need in the third world, which implies always more efficient and reliable engineering tools to be developed. As a major global challenge it is important to make children aware of this problem for understanding (i) what safe drinking water is, (ii) how ingenious techniques are developed for this purpose and (iii) the role of microfluidics in this area. This paper focuses on different microfluidic-based techniques to separate and detect pathogens in drinking water that have been adapted to be performed by a young audience in a simplified, recreational and interactive way.

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The Risk Assesment and Impact of Hygienic Conditions on the Development of Legionella Spp. In Water Systems of Public and Tourist Facilities

Bešić

Obradović

Dautbegović³

et al. 2017

Int. J. Res. Granthaalayah

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A large number of different diseases are associated with the consumption of hygienic defective water and the use of water for maintaining personal hygiene. Recreational waters, water in systems intended for cooling and heating of public buildings are also significant. Among these diseases, the most significant is legionellosis, the disease which has become more and more important in the whole world in recent years. The aim of this paper was to evaluate the connection of hygienic conditions in public buildings with the appearance of genus Legionella in drinking and bathing water, as well as water from outside and inside fountains, pools and water from cooling and heating systems for public buildings. Obtained results are also used to evaluate the risk of developing legionellosis in the population of Bosnia and Herzegovina and among foreign tourists. The presence of Legionella spp. was analysed in 238 samples of water taken from faucets in rooms of buildings intended for people's accommodation, in open and closed fountains, pools, cooling and water systems. Hygienic conditions in the facilities were assessed using survey questionnaire and complemented with legionella findings. This study showed the direct correlation between hygienic conditions in public and tourist facilities with the presence of Legionella spp. in the waters taken in these objects.

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Detection of Pathogens in Water Using Micro and Nano-Technology

Cited by 4 publications

References 393 publications

An innate immune system-mimicking, real-time biosensing of infectious bacteria

An innate immune system-mimicking, real-time biosensing of infectious bacteria

Angry pathogens, how to get rid of them: introducing microfluidics for waterborne pathogen separation to children

The Risk Assesment and Impact of Hygienic Conditions on the Development of Legionella Spp. In Water Systems of Public and Tourist Facilities

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