Detection of PBP2a (penicillin-binding protein 2a) and mecA gene in methicillin resistant staphylococci originated from animals

Abstract

For the purpose of detecting methicillin (oxacillin) resistance in staphylococcal strains, in a number of microbiological laboratories only disc diffusion method with cefoxitin and/or oxacillin discs is used. Besides this method, it is desirable to determine MIC values for cefoxitin and/or oxacillin. After examination by disc diffusion and dilution methods, latex agglutination is used for the detection of PBP2a and PCR is used for the detection of mecA gene. Use of PCR is not possible in a large number of diagnostic laboratories and as method of choice, latex agglutination test for rapid detection of PBP2a is recommended. In this investigation, as confirmatory methods, latex agglutination and PCR were used for strains that were resistant to oxacillin and/or cefoxitin by disc diffusion and broth microdilution methods. In total, 14 strains of coagulase-negative staphylococci originating from clinical specimens of cats, dogs and chicken were examined. Among isolated strains, it was established that the dominating species was Staphylococcus haemolyticus with 11 isolated strains. Other isolated species were Staphylococcus epidermidis, Staphylococcus capitis and Staphylococcus vitulinus, each with one isolated strain. For all 14 strains, oxacillin MIC values ranged from 0.5 μg/mL to >64 μg/mL and cefoxitin MIC values ranged from 1 μg/mL to >256 μg/mL. Positive agglutination reaction by latex agglutination test was recorded in 13 out of 14 strains. The PCR assay for mecA gene was positive in 12 investigated strains. [Projekat Ministarstva nauke Republike Srbije, br. 31079

Deformaciona Merenja I Analiza Objekata Saobraćajne Infrastrukture

“…Uloga inženjerske geodezije kod izgradnje objekata je da planiranjem, organizacijom i izvršavanjem odgovarajućih geodetskih radova obezbedi prostorno lociranje i ostvarivanje geomerije izgrađenog objekta saglasno projektovanoj u granicama tolerancija građenja, radi uspešne i efikasne eksploatacije [3].…”

“…Geodetski radovi za potrebe projektovanja i izgradnje objekata izvršavaju se u sledećim fazama [3]:  Izrada programa geodetskih radova ili projektnog zadatka  Izrada projekta geodetskih radova  Realizacija projekta geodetskih radova  Izrada elaborata o realizaciji projekta geodtskih radova. Svi geodetski radovi koje je potrebno izvršiti za potrebe projektovanja i izgradnje objekata mogu se razvrstati na sledeći način [3]:…”

Investigation of Presence of Methicillin Resistant Staphylococci in Students of the Faculty of Veterinary Medicine at the University of Belgrade

“…Th e resistance to methicillin was fi rst detected in Staphylococcus aureus in Great Britain in 1961 (Ašanin et al, 2012;Ćirković et al, 2015;Kluytmans et al, 1997). Th is type of resistance emerged only two years aft er methicillin, a semisynthetic penicillin preparation resistant to an inducible enzyme -penicillinase, was introduced into clinical practice.…”