Detection of phosphatidylserine by using liquid crystal supported on the gold-deposited waveform surfaces with the annexin V-based signal enhancement

Duong, Duong Song Thai; Jang, Chang‐Hyun

doi:10.1016/j.microc.2022.108176

Cited by 3 publications

(1 citation statement)

References 41 publications

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“…Based on the current state of research, methods for quantitative analysis of PS expression levels on the surface of cells are mainly categorized into the following approaches. The first approach involves the utilization of flow cytometry, , enzyme-linked immunosorbent assay, high-performance liquid chromatography (HPLC), , liquid mass spectrometry, , electrochemistry, , and additional techniques − for the separation and quantitative analysis of PS on cellular membranes. The second method is the fluorescence real-time imaging quantitative technique, which typically employs PS detection probes such as fluorescently labeled annexin V and zinc(II) dipyridinamine complex (ZnDPA) for signal “always on” detection. − Subsequently, the fluorescent probe P-IID was constructed by using the intramolecular fluorescent photoreceptor substitution mechanism with ZnDPA as the identification site.…”

Section: Introductionmentioning

confidence: 99%

Quantification of Phosphatidylserine Molecules on the Surface of Individual Cells Using Single-Molecule Force Spectroscopy

Li,

Zhang,

Wang

et al. 2024

Anal. Chem.

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Identification of the phosphatidylserine (PS) discrepancies occurring on the cellular membrane during apoptotic processes is of the utmost importance. However, monitoring the quantity of PS molecules in real-time at a single-cell level currently remains a challenging task. Here, we demonstrate this objective by leveraging the specific binding and reversible interaction exhibited by the zinc(II) dipyridinamine complex (ZnDPA) with PS. Lipoic acid-functionalized ZnDPA (LP-ZnDPA) was subsequently immobilized onto the surface of an atomic force microscopy cantilever to form a force probe, ALP−ZnDPA, enabling a PS-specific dynamic imaging and detection mode. By utilizing this technique, we can not only create a heat map of the expression level of PS with submicron resolution but also quantify the number of molecules present on a single cell's surface with a detection limit of 1.86 × 10 4 molecules. The feasibility of the proposed method is demonstrated through the analysis of PS expression levels in different cancer cell lines and at various stages of paclitaxel-induced apoptosis. This study represents the first application of a force probe to quantify PS molecules on the surface of individual cells, providing insight into dynamic changes in PS content during apoptosis at the molecular level and introducing a novel dimension to current detection methodologies.

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Section: Introductionmentioning

confidence: 99%

Quantification of Phosphatidylserine Molecules on the Surface of Individual Cells Using Single-Molecule Force Spectroscopy

Li,

Zhang,

Wang

et al. 2024

Anal. Chem.

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Amphiphilic ligand‐doped liquid crystal‐based detection of Hg²⁺ ions on polyimide surface with alkyl pendent groups

Majeed,

Siddiqi,

Urooj

et al. 2024

Polymer International

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In this study, amphiphilic thiosemicarbazone was used to align liquid crystal (4‐cyano‐4′‐pentylbiphenyl) in a homeotropic way on polyimide containing alkyl pendent groups (AHDPI), which was coated on a glass slide. The amphiphilic ligands 2‐(4‐(dodecyloxy)benzylid‐ene)hydrazine‐1‐carbothioamide (DT) and 2‐(1‐dodecyl‐2‐oxoindolin‐3‐ylidene)hydrazine‐1‐carbothioamide (IT) were doped with liquid crystal (LC) to develop LC‐based sensors to detect Hg2+ ions in water. The selective interaction of carbothioamide with Hg2+ ions triggered the orientation transition of LC from homeotropic to parallel alignment and gave dark to bright optical signal at the LC/aqueous interphase. Self‐immobilization of thiosemicarbazone‐based ligands on AHDPI‐coated glass slide can be used to detect Hg2+ ions with high sensitivity. The limit of detection with DT and IT was found to be 0.5 and 0.25 μmol L−1, respectively. Density functional studies were carried out to study the interaction of the thiosemicarbazone ligands with mercuric ions, resulting in highly negative binding energies of −1.55 and −2.06 eV for DT and IT with Hg2+ ions, respectively. The chemical and thermal stability (up to 268 °C) of the AHDPI coated on glass slide made it reusable at least twice for sensor fabrication. This provides a quicker and cheaper alternative to traditional methods of sensor fabrication. © 2024 Society of Chemical Industry.

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Sensitive detection of exosomes by an electrochemical aptasensor based on double nucleic acid amplifications

Zhang,

Chen,

Xie

et al. 2024

Microchemical Journal

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Detection of phosphatidylserine by using liquid crystal supported on the gold-deposited waveform surfaces with the annexin V-based signal enhancement

Cited by 3 publications

References 41 publications

Quantification of Phosphatidylserine Molecules on the Surface of Individual Cells Using Single-Molecule Force Spectroscopy

Quantification of Phosphatidylserine Molecules on the Surface of Individual Cells Using Single-Molecule Force Spectroscopy

Amphiphilic ligand‐doped liquid crystal‐based detection of Hg²⁺ ions on polyimide surface with alkyl pendent groups

Sensitive detection of exosomes by an electrochemical aptasensor based on double nucleic acid amplifications

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Detection of phosphatidylserine by using liquid crystal supported on the gold-deposited waveform surfaces with the annexin V-based signal enhancement

Cited by 3 publications

References 41 publications

Quantification of Phosphatidylserine Molecules on the Surface of Individual Cells Using Single-Molecule Force Spectroscopy

Quantification of Phosphatidylserine Molecules on the Surface of Individual Cells Using Single-Molecule Force Spectroscopy

Amphiphilic ligand‐doped liquid crystal‐based detection of Hg2+ ions on polyimide surface with alkyl pendent groups

Sensitive detection of exosomes by an electrochemical aptasensor based on double nucleic acid amplifications

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Amphiphilic ligand‐doped liquid crystal‐based detection of Hg²⁺ ions on polyimide surface with alkyl pendent groups