Detection of picobirnaviruses in vervet monkeys ( Chlorocebus sabaeus ): Molecular characterization of complete genomic segment-2

Gallagher, Christa A.; Navarro, Ryan; Cruz, Katalina; Aung, Meiji Soe; Ng, Agnes; Bajak, Edyta; Beierschmitt, Amy; Lawrence, Matthew S.; Dore, Kerry M.; Ketzis, Jennifer; Malik, Yashpal Singh; Kobayashi, Nobumichi; Ghosh, Souvik

doi:10.1016/j.virusres.2016.12.021

Cited by 14 publications

(23 citation statements)

References 20 publications

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“…The screening results were confirmed by sequencing the~201 bp PCR amplicons of gene segment-2 of the mongoose PBV GI strains. None of the PBV positive mongooses exhibited clinical signs of diarrhea, corroborating previous reports on asymptomatic PBV infection in wildlife [5,6,13,15]. The mongooses were trapped in both urban and wild habitats, and most likely came into contact with humans and various animal species (cats, dogs, livestock, rodents, and vervet monkeys), which may have increased their chances of exposure and PBV infection ( Figure 2).…”

Section: Detection Of Pbvs In Mongoosessupporting

confidence: 87%

“…Until recently, most studies on genetic diversity of PBVs were limited to analyses of short nt sequences (~201 bp of gene segment-2) which may not be sufficient to obtain conclusive information on the putative RdRps, or genetic make-up of PBVs [5,6,12]. However, by applying a non-specific primer-based amplification method, or next-generation sequencing, it has been possible to obtain the whole genomes, or the complete/nearly complete gene segment-2 sequences of several PBV strains from various host species, providing important insights into the genetic diversity and evolution of PBVs [1,2,10,[13][14][15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

“…Although PBVs have been detected in a wide variety of host species including wildlife [5,6,10,13,15,20], there have been no reports on PBVs from the mongoose so far. In the present study, we report for the first time detection and molecular characterization of complete/nearly complete gene segment-2 (full length minus partial 3 -untranslated region (UTR)) of PBV strains from the small Indian mongoose (Urva auropunctata) on the Caribbean island of St. Kitts.…”

Section: Introductionmentioning

confidence: 99%

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Detection and Molecular Characterization of Picobirnaviruses (PBVs) in the Mongoose: Identification of a Novel PBV Using an Alternative Genetic Code

Kleymann

Becker

Malik

et al. 2020

Viruses

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We report high rates of detection (35.36%, 29/82) of genogroup-I (GI) picobirnaviruses (PBVs) in non-diarrheic fecal samples from the small Indian mongoose (Urva auropunctata). In addition, we identified a novel PBV-like RNA-dependent RNA polymerase (RdRp) gene sequence that uses an alternative mitochondrial genetic code (that of mold or invertebrate) for translation. The complete/nearly complete gene segment-2/RdRp gene sequences of seven mongoose PBV GI strains and the novel PBV-like strain were obtained by combining a modified non-specific primer-based amplification method with conventional RT-PCRs, facilitated by the inclusion of a new primer targeting the 3′-untranslated region (UTR) of PBV gene segment-2. The mongoose PBV and PBV-like strains retained the various features that are conserved in gene segment-2/RdRps of other PBVs. However, high genetic diversity was observed among the mongoose PBVs within and between host species. This is the first report on detection of PBVs in the mongoose. Molecular characterization of the PBV and PBV-like strains from a new animal species provided important insights into the various features and complex diversity of PBV gene segment-2/putative RdRps. The presence of the prokaryotic ribosomal binding site in the mongoose PBV genomes, and analysis of the novel PBV-like RdRp gene sequence that uses an alternative mitochondrial genetic code (especially that of mold) for translation corroborated recent speculations that PBVs may actually infect prokaryotic or fungal host cells.

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Section: Detection Of Pbvs In Mongoosessupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detection and Molecular Characterization of Picobirnaviruses (PBVs) in the Mongoose: Identification of a Novel PBV Using an Alternative Genetic Code

Kleymann

Becker

Malik

et al. 2020

Viruses

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“…In this study, picobirnavirus sequences were detected from seven out of ten stool samples of green monkeys with diarrhea, suggesting a high picobirnavirus infection ratio in African green monkeys. Picobirnavirus strain PBV/Simian/KNA/08873/2015 RdRp sequence detected from an African green monkey in the Caribbean region (Gallagher et al, 2017) had 69.93% nucleotide similarity (92% coverage) and 63.27% amino acid identity (100% coverage) with that of the picobirnavirus in this study, illustrating the great diversity among picobirnaviruses in African green monkeys. The high nucleotide similarity (93%) of picobirnavirus RdRp detected in this study with that of human picobirnavirus GPBV6C2 found in a stool specimen from a patient with diarrhea indicated that African green monkey could be a reservoir of human picobirnavirus.…”

Section: Discussionmentioning

confidence: 54%

“…Oberste et al (2013) investigated three picornavirus genera (Enterovirus, Parechovirus, and Sapelovirus) in African green monkeys at the Dazark Zoo of Bangladesh, but none was detected. Gallagher et al(2017) detected simian picobirnaviruses in fecal samples from diarrhea-free African green monkeys of the Caribbean region. However, little research has reported the virome in stools of African green monkeys.…”

Section: Introductionmentioning

confidence: 93%

Virome diversity analysis reveals novel enteroviruses and a human picobirnavirus in stool samples from African green monkeys with diarrhea

Xin

Qin

et al. 2020

Infection, Genetics and Evolution

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It is important to identify viruses in animals because most infectious diseases in humans are caused by viruses of zoonotic origin. African green monkey is a widely used non-human primate model in biomedical investigations. In this study, total RNAs were extracted from stool samples of 10 African green monkeys with diarrhea. High-throughput sequencing was used to characterize viromes. PCR and Sanger sequencing were used to determine the full genome sequences. Great viral diversity was observed. The dominant viruses were enteroviruses and picobirnaviruses. Six enterovirus genomes and a picobirnavirus RNA-dependent RNA polymerase sequence were characterized. Five enteroviruses belonged to two putative new genotypes of species Enterovirus J. One enterovirus belonged to EV-A92. The picobirnavirus RNA-dependent RNA polymerase sequence had the highest nucleotide similarity (93.48%) with human picobirnavirus isolate GPBV6C2. The present study helped to identify the potential zoonotic viruses in African green monkeys. Further investigations are required to elucidate their pathogenic roles in animals and humans.

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Picobirnavirus

Kumar

Mascarenhas

Ghosh

et al. 2020

Livestock Diseases and Management

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Detection of picobirnaviruses in vervet monkeys ( Chlorocebus sabaeus ): Molecular characterization of complete genomic segment-2

Cited by 14 publications

References 20 publications

Detection and Molecular Characterization of Picobirnaviruses (PBVs) in the Mongoose: Identification of a Novel PBV Using an Alternative Genetic Code

Detection and Molecular Characterization of Picobirnaviruses (PBVs) in the Mongoose: Identification of a Novel PBV Using an Alternative Genetic Code

Virome diversity analysis reveals novel enteroviruses and a human picobirnavirus in stool samples from African green monkeys with diarrhea

Picobirnavirus

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