Detection of Plant Pathogen Spores of Economic Significance on Pollen Trap Slides

Brittain, Ian; Selby, Katherine; Taylor, Moray; Mumford, R. A.

doi:10.1111/jph.12129

Cited by 4 publications

(5 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although spore traps have been largely used in epidemiology, so far, most studies have been performed at local scale (i.e., with spore traps usually at ground level, within or in the proximity of forest stands or crop fields). The few studies using air samplers set up on rooftops or towers for the detection of plant pathogens were generally performed at only a few sites and dates, and none reported the detection of forest pathogens (Brittain et al ., 2013; Banchi et al ., 2018; Migliorini et al ., 2019). Our study takes a step further by clearly positioning this approach in an epidemiological surveillance perspective using an established network of traps, such as those operated for pollen monitoring, and by providing data that demonstrate its feasibility and applicability for forest pathogens.…”

Section: Discussionmentioning

confidence: 99%

“…The first issue was to confirm that forest pathogenic fungi can be detected in aerobiological samples from pollen monitoring networks, despite the location of traps on rooftops in urban environments (providing regional sampling but with probably very low densities of spores). Although a few studies report the detection of some crop pathogens in rooftop traps (Brittain et al ., 2013; Banchi et al ., 2018; Migliorini et al ., 2019), they do not document the presence of forest pathogens. To answer this question, we used two molecular detection methods, a metabarcoding approach and specific real‐time PCR.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Combining permanent aerobiological networks and molecular analyses for large‐scale surveillance of forest fungal pathogens: A proof‐of‐concept

Aguayo¹,

Husson

Chancerel

et al. 2020

Plant Pathology

View full text Add to dashboard Cite

Forest disease management relies principally on a preventive approach in which epidemiological surveillance plays a crucial role. However, efficient and cost-effective surveillance methods are not currently available for large spatial scales. Nevertheless, aerobiological networks have been set up for several decades in many countries to monitor pollen dispersal and provide real-time assessments of allergenic risk. Here, we suggest that the same approach could be used for the surveillance of forest pathogens. Using molecular methods, we analysed samples from 12 sites of the French aerobiological network, at different dates. Both metabarcoding by high-throughput sequencing (using two markers and two different bioinformatics approaches) and real-time PCR targeting eight important forest pathogens were conducted. To validate the approach, temporal and spatial trends of spore detection were compared with field disease data. The metabarcoding approach demonstrated that many fungal plant pathogens could be found in aerobiological samples. Moreover, five of the eight targeted forest pathogens were detected by real-time PCR, with temporal and spatial trends of spore capture consistent with field data. In particular, Hymenoscyphus fraxineus was detected at high frequency in aerobiological samples in the areas where ash dieback has been present for the longest period of time, and at lower frequency in areas with more recent invasion. Spore detection of seasonal pathogens showed a temporal pattern similar to that of disease reports. Overall, our study provides a proof of concept that permanent aerobiological networks combined with molecular methods may provide a useful tool for large-scale surveillance of forest pathogens.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Combining permanent aerobiological networks and molecular analyses for large‐scale surveillance of forest fungal pathogens: A proof‐of‐concept

Aguayo¹,

Husson

Chancerel

et al. 2020

Plant Pathology

View full text Add to dashboard Cite

show abstract

“…However, due to the climate changes in the Mediterranean region, the pathogen sporulation and dispersal may be favored, leading to an increase of the disease incidence (Santini & Ghelardini 2015). As nowadays new spore trapping tools coupled with sensitive molecular methods give the opportunity to ef-ficiently collect and detect airborne fungal inoculum (Brittain et al 2013), here we propose to analyze the seasonal spore disper-sal of C. pinea in a P. radiata plantation, and its relation to local climatic conditions by using a rotating-arm spore trapping method combined with qPCR.…”

Section: Introductionmentioning

confidence: 99%

Detection and quantification of the air inoculum of Caliciopsis pinea in a plantation of Pinus radiata in Italy

Botella¹,

Bačová²,

Dvořák³

et al. 2019

iForest

View full text Add to dashboard Cite

Caliciopsis pinea has been historically described as a secondary pathogen of pines. However, it has recently been associated with severe damages on Pinus radiata in Italy. Our study focused on the description of the seasonal spore dispersal of C. pinea and its relation to meteorological conditions (temperature, leaf wetness, relative humidity and precipitations). For this experiment one infected P. radiata plantation was sampled in Tuscany (Italy). A rotating arm spore trap together with a weather station were installed to sample the aerospora for 24 h every week from May to November 2016. Exposed tapes from spore traps were directly analyzed after DNA extraction by qPCR using specific primers and TaqMan MGB probe. The study shows an irregular occurrence of the inoculum of C. pinea throughout the whole sampling period with peak levels in mid-June and early August. The statistical analysis of the DNA and climatic data clearly show the strong influence of precipitation on the spore production of this pathogen. Furthermore, the very low detection limit of the qPCR experiment shows the efficacy and suitability of rotating arm spore traps for early detection of this pathogen.

show abstract

“…For an accurate measurement of the airborne spore concentration, spore traps can be used (Hirst 1953;Aylor et al 2001;Brittain et al 2013). The measured spore concentration in the atmosphere is the result of different processes, starting with spore production, spore release or removal from the substrate and transport (Lyon et al 1984).…”

Section: Introductionmentioning

confidence: 99%

Temporal variation in airborne spore concentration of Chrysomyxa rhododendri: correlation with weather conditions and consequences for Norway spruce infection

Ganthaler

Mayr

2015

Forest Pathology

View full text Add to dashboard Cite

The fungal pathogen Chrysomyxa rhododendri undergoes a host shift between Rhododendron spp. and Picea abies and has a considerable negative impact on the latter by infecting the new sprouting needles and reducing photosynthesis, growth and seedling survival. Repeated high infection rates in the Central European Alps were reported in recent years, and although the life cycle of the pathogen is well understood, knowledge on temporal patterns of host infection, spore dispersal and influence of weather conditions is limited. We analysed the period of needle infection by a staggered application of fungicide on twigs of Norway spruce. Seasonal and diurnal patterns of spore dispersal were investigated using a Burkard volumetric spore trap, and airborne spore concentrations were correlated with several weather parameters. Needle infection occurred within 3 weeks (19 June-9 July). Basidiospores (infecting Norway spruce) were trapped from May to July, and airborne spore concentration was positively correlated with air temperature, global radiation and wind speed, and negatively with air humidity and precipitation. Aeciospores (infecting rhododendrons) appeared from August to October, and high concentrations were significantly associated with rain events. Needle infection started with bud sprouting and was limited either by decreasing spore concentrations or morphological and chemical differentiation of the needles, which prevented infection. Both spore types showed distinct periods of dispersal based on the life cycle of the pathogen, and the variability in concentration within these periods was explained by local weather conditions. The correlation analysis indicated different mechanisms of spore dispersal for both spore types and, with respect to global warming, more favourable conditions for the pathogen in future. The results can explain the high year-and site-specific variation in Chrysomyxa infection intensities of P. abies and may be helpful for developing successful strategies to control the pathogen and protect alpine spruce forests.

show abstract

Detection of Plant Pathogen Spores of Economic Significance on Pollen Trap Slides

Cited by 4 publications

References 19 publications

Combining permanent aerobiological networks and molecular analyses for large‐scale surveillance of forest fungal pathogens: A proof‐of‐concept

Combining permanent aerobiological networks and molecular analyses for large‐scale surveillance of forest fungal pathogens: A proof‐of‐concept

Detection and quantification of the air inoculum of Caliciopsis pinea in a plantation of Pinus radiata in Italy

Temporal variation in airborne spore concentration of Chrysomyxa rhododendri: correlation with weather conditions and consequences for Norway spruce infection

Contact Info

Product

Resources

About