Detection of Plasmid-Mediated Mobile Colistin Resistance Gene (mcr-1) in Enterobacterales Isolates from a University Hospital

Anan, Mera Mohammed Galal; El-Seidi, Eman Ahmed; Mostafa, Mohammed; Rashed, Laila Ahmed; El-Wakil, Doaa Mahdy

doi:10.2147/idr.s318787

Cited by 10 publications

(20 citation statements)

References 40 publications

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“…However, higher than detected by El-Sokkary et al 24 study where 2.79% CRE was colistin resistant. However, our results are much lesser than that regarding Anan et al 25 study, who detect 75% of K. pneumoniae isolates were colistin resistant by BMD.…”

Section: Table (5): Antibiotic Susceptibility Of Cpkp In Relation Tocontrasting

confidence: 92%

Colistin Susceptibility among Carbapenem Resistant Klebsiella pneumoniae Isolated from Menoufia University Hospitals

Elaskary¹,

Badawy²

2023

The Egyptian Journal of Hospital Medicine

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Background: Infections by CRKp represents a major health problem. Carbapenemases have been frequently reported in CRKp. Limited antimicrobials are still effective for CRKp like tigecycline, ceftazidime/avibactam, as well, polymyxins including colistin. With the increased use of colistin, Col R k. pneumoniae carbapenemase (KpC)producing isolates are documented worldwide. Objectives: The aim of the current study is to investigate carbapenemases and to determine colistin susceptibility and mcr genes among CRKp. Material and methods: k. pneumoniae were identified by conventional methods and API20E. Carbapenem and colistin resistance were screened by disc diffusion method. Confirmatory combined mCIM/eCIM testing was used for carbapenemases detection. BMD and CBDE tests were used for colistin susceptibility detection. Multiplex PCRs were done for carbapenemases genes (blaNDM-1, blaOXA48, blaIMP blaVIM, blaKPC) and colistin resistance genes (mcr1, 2, 3, 4 and 5 genes) detection. Results: Out of CRKp, 68.9% were positive carbapenemases by mCIM/eCIM testing, 33.3% of them were MBLs and 66.7% were serine carbapenemases. the most frequent carbapenemases genes were blaKPC and blaOXA-48. About 16.3% of CRKp were colistin resistant by BMD test. There was fair agreement of CBDE test in relation to BMD for colistin susceptibility detection. The mcr1 gene was detected in 9.1% of CRKp. Conclusions: The increase detection of carbapenemases and colistin resistance among k. pneumoniae isolates obligate us for efficient use of prevention and control protocols to decrease the multidrug resistance in hospital and community environments.

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Section: Table (5): Antibiotic Susceptibility Of Cpkp In Relation Tocontrasting

confidence: 92%

Colistin Susceptibility among Carbapenem Resistant Klebsiella pneumoniae Isolated from Menoufia University Hospitals

Elaskary¹,

Badawy²

2023

The Egyptian Journal of Hospital Medicine

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“…These results are similar to studies in Egypt [23,25]. On the other hand, other studies showed that some negative isolates in PCR exhibited phenotypic colistin resistance in MIC [24,26].…”

Section: A Single E Coli Isolates Harbored the Mcr-1 Genesupporting

confidence: 90%

Colistin resistance genes inEscherichia coliisolated from patients with urinary tract infections

Al Momani,

Ata,

Maslat

2024

Preprint

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Introduction: Antimicrobial resistance is alarmingly high because it happens in humans, environment, and animal sectors from a "One Health" viewpoint. Due to the fact, that Escherichia coli (E. coli) is broadly disseminated in all sectors, the food web and the environment may have a role in carrying colistin resistance genes from animals to humans. The rise of plasmid-mediated mobile colistin resistance (MCR) genes threatens colistin efficacy, which is the last line to remedy Gram-negative infections multidrug resistance (MDR). Objectives: The current study aimed to investigate development of colistin resistance (MCR 1-5) genes between E. coli isolated from patients with urinary tract infections (UTI) in Jordan. Methods: E. coli (n=132) isolated were collected from urine specimens. E. coli isolated from human UTI patients were examined for genes resistance to colistin MCR (1-5). All isolates were investigated against 20 antimicrobials utilizing the standard disk diffusion method. For analysis of colistin resistance, the broth microdilution technique was employed. In addition, the MCR (1-5) genes were detected by multiplex PCR assay. Results: Out of 132 isolates, one isolate was colistin-resistant, having a minimum inhibitory concentration of 8 μg/mL and possessing the MCR -1 gene. A total of 132 E. coli isolates showed high resistance against penicillin, amoxicillin, cephalexin, nalidixic acid, tetracycline, and cefepime in the percentages of 100%, 79.55%, 75.76%, 62.88%, 58.33%, and 53.79%, respectively. However, resistance was lowest towards fosfomycin (6.06%), florfenicol (10.61%), and chloramphenicol (15.91%). Significant differences were observed between E. coli isolated from pediatrics and those isolated from adults. Conclusion: This is the first report on the presence of the plasmid-coded MCR-1 gene recovered from E. coli from a patient with UTIs in Jordan. That is threatening as colistin is the last line used for infections induced by MDR gram-negative bacteria. There is a crucial need for control and harsh utilization of antibiotics to control and prevent the emergence and prevalence of colistin resistance genes.

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“…Broth micro dilution (BMD) was recommended as the standard procedure. This standard technique is quite laborious, time-consuming, difficult to interpret, susceptible to mistakes, not suitable for most laboratories 8, 9 . Currently, agar dilution and colistin broth disk elution (CBDE) are acceptable methods 10 .…”

Section: Introductionmentioning

confidence: 99%

Assessment of Colistin Susceptibility among Carbapenem-Resistant Clinical Isolates

Ajlan¹,

Elmahdy

Sleem

2022

Egyptian Journal of Medical Microbiology

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Background: Colistin is the last treatment option for infections caused by carbapenem resistant Gram-negative bacilli (CRGNB). The increasing spread of chromosomally encoded and plasmid-mediated colistin resistance made colistin susceptibility assessment a necessity. Objectives: Assessment of colistin susceptibility in CRGNB by broth micro dilution method (BMD), as the standard method and colistin broth disk elution method (CBDE), as a substitute procedure with genotypic determination of plasmid mediated colistin resistance (mcr) genes. Methodology: CRGNB were collected and identified by conventional methods. Testing carbapenemase production by modified Carbapenem Inactivation Method (mCIM) and colistin susceptibility (by BMD and CBDE) were done and results were interpreted regarding CLSI ( 2022) guidelines followed by genotypic detection of mcr-1, -2, -3, -4, and -5 genes by multiplex PCR. Results: 155 out of 308 GNB (50.3%) were carbapenem resistant. Among them, 129 (83.2%) isolates were carbapenemase positive by mCIM. Colistin susceptibility testing by BMD revealed 43 out of 155 CRGNB isolates (27.7%) were colistin resistant. Sensitivity, specificity, PPV, NPV and accuracy of CBDE were 99.09%, 93.33%, 97.32% 97.67%, 97.42% respectively with almost perfect agreement with BMD. By PCR, only 3 CRGNB isolates (6.98%) carried mcr-1 while other mcr genes were not detected at all. Conclusion: Colistin resistance rate among CRGNB is concerning, causing serious and even deadly infections so prospective surveillance is essential. Broth disk elusion method is a simple, non-expensive reliable option to test colistin susceptibility.

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Detection of Plasmid-Mediated Mobile Colistin Resistance Gene (mcr-1) in Enterobacterales Isolates from a University Hospital

Cited by 10 publications

References 40 publications

Colistin Susceptibility among Carbapenem Resistant Klebsiella pneumoniae Isolated from Menoufia University Hospitals

Colistin Susceptibility among Carbapenem Resistant Klebsiella pneumoniae Isolated from Menoufia University Hospitals

Colistin resistance genes inEscherichia coliisolated from patients with urinary tract infections

Assessment of Colistin Susceptibility among Carbapenem-Resistant Clinical Isolates

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