Detection of Point Prevalence and Assemblages of <i>Giardia</i> spp. in Dairy Calves and Sika Deer, Northeast China

Ma, Ning; Wang, Haoxian; Tao, Wei-Fu; Xue, Nian-Yu; Bai, Jin-Yang; Zhao, Quan; Jiang, Jing; Lyu, Chuang

doi:10.1089/vbz.2020.2757

Cited by 4 publications

(2 citation statements)

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“…In addition, the overall prevalence in the preweaned calves was significantly lower than that in the other age groups and that in postweaned calves was significantly higher than that in the other age groups in all the samples, which does not reflect the decrease in G. duodenalis infection rates with increasing age as reported in the literature 42 . The high prevalence of G. duodenalis in postweaned calves has also been reported in China 4 , 27 , 29 , Norway 57 , Germany 58 , USA 59 , 60 , and Canada 61 . However, there are also several studies reporting a relatively high prevalence of G. duodenalis infection in preweaned calves 24 , 28 , 31 , 36 , 37 , 39 – 43 .…”

Section: Discussionmentioning

confidence: 67%

“…In China, the prevalence of G. duodenalis in this study was only lower than the prevalence of G . duodenalis in cattle in Shanghai (60.1%, 492/818) 32 , Sichuan (41.2%, 126/306) 34 , and Guangdong (74.2%, 288/388) 38 and higher than the prevalence data reported in Xinjiang (13.4%, 69/514) 4 ; Ningxia (4.38%, 74/1688) 37 and (2.12%, 29/1366) 24 ; Heilongjiang (5.2%, 42/814) 25 , 26 , (4.98%, 16/321) 27 and (15.4%, 8/52) 28 ; Jilin (6.63%, 25/377) 28 ; Liaoning (8.4%, 19/226) 28 ; Hubei ( 22.7%, 77/339) 29 ; Shandong (13.04%, 9/69, PCR) and (18.84%, 13/69, rapid kit) 30 ; Shaanxi (18.87%, 70/371) 31 ; Sichuan (9.4%, 26/278) 33 ; Beijing (1.70%, 14/822) 35 ; Gansu (1.0%, 14/1414) 36 and (2.63%, 33/1257) 37 ; Guangdong (2.2%, 31/1440) 39 ; Hebei and Tianjin (4.7%, 49/1040) 40 ; Henan (7.2%, 128/1777) 41 ; Jiangsu (20.6%, 281/1366) 42 ; Jiangxi (9.2%, 52/566) 43 ; Qinghai (10%, 39/389) 45 ; Tibet (3.8%, 17/442) 46 ; Yunnan (10.49%, 41/ 391) 47 ; Taiwan (19.87%, 31/156) 48 and that reported in the only survey on G. duodenalis in Inner Mongolia (9.3%, 10/108) 44 . However, it is difficult to compare prevalence data because they are influenced by a range of factors, including study design, diagnostic method, geographical conditions, total number of samples, age of animals, and sampling season 4 .…”

Section: Discussionmentioning

confidence: 69%

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Prevalence and molecular characterization of Giardia duodenalis in dairy cattle in Central Inner Mongolia, Northern China

Zhao,

Zhang,

Han

et al. 2023

Sci Rep

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Giardia duodenalis is a gastrointestinal protozoan ubiquitous in nature. It is a confirmed zoonotic pathogen, and cattle are considered a source of giardiasis outbreaks in humans. This study aimed to evaluate the prevalence and multilocus genotype (MLG) of G. duodenalis in dairy cattle in Central Inner Mongolia. This study was based on the small subunit ribosomal RNA (SSU rRNA), glutamate dehydrogenase (gdh), triosephosphate isomerase (tpi), and beta-giardin (bg) genes of G. duodenalis. DNA extraction, polymerase chain reaction (PCR), and sequence analysis were performed on 505 dairy cattle fecal samples collected in 2021 from six sampling sites and four age groups in Central Inner Mongolia to determine the prevalence and MLG distribution of G. duodenalis. The PCR results of SSU rRNA revealed that the overall prevalence of G. duodenalis was 29.5% (149/505) and that the overall prevalence of the diarrhea and nondiarrhea samples was 31.5% (46/146) and 28.5% (103/359), respectively; the difference was not significant (p > 0.05). SSU rRNA sequence analysis revealed that G. duodenalis assemblage E (91.1%, 133/146) was primarily detected and that assemblage A (8.9%, 13/146) was detected in 13 samples. The G. duodenalis—positive samples were PCR amplified and sequenced for gdh, tpi, and bg, from which 38, 47, and 70 amplified sequences were obtained, respectively. A combination of G. duodenalis assemblages A and E were detected in seven samples. Multilocus genotyping yielded 25 different assemblage E MLGs, which formed six subgroups. To the best of our knowledge, this is the first report regarding G. duodenalis infection in dairy cattle in Inner Mongolia, China. This study revealed that Inner Mongolian cattle pose a risk of giardiasis transmission to humans and that the distribution of local cattle G. duodenalis assemblage E MLGs is diverse. The findings of this study can bridge the knowledge gap in the molecular epidemiological investigation of giardiasis in Central Inner Mongolia.

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