Detection of Polycyclic Aromatic Hydrocarbon-DNA Adducts in Placental DNA Samples by Room-Temperature Solid-Matrix Phosphorescence

Hurtubise, Robert J.; Thompson, Allison L.; Weston, Ainsley; Manchester, David K.

doi:10.1080/00032710802209219

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2011

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“…7 Long-lived room-temperature phosphorescence has found use in studies of DNA adducts with carcinogenic polyaromatic hydrocarbons. 8 Concomitant with interest in phosphorescence, instrumentation has developed in parallel beginning with Becquerel's simple phosphoroscope and becoming increasingly sophisticated, with advances in electronics and light sources. Variations on Becquerel's mechanical device range from spinning notched wheels (chopper) 9 to slotted tin cans as well as rotating samples.…”

Section: Introductionmentioning

confidence: 99%

Laser Phosphoroscope and Applications to Room-Temperature Phosphorescence

et al. 2011

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A simple phosphoroscope with no moving parts is described. In one scan the total luminescence, the long-lived phosphorescence, and the short-lived fluorescence can be determined. A 50% duty cycle excitation from a diode laser is used to excite the sample, and from the digitized waveform the phosphorescence is extracted from the off period, the total emission from the full cycle, and the fluorescence from the on period corrected for the phosphorescence contribution. The performance of the system is demonstrated using room-temperature phosphorescence of organic dyes in boric acid glasses, a multi-emissive boron-polymer dye, and a europium chelate.

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Section: Introductionmentioning

confidence: 99%