Detection of polycyclic aromatic hydrocarbons in skin oil obtained from roofing workers

Wolff, Mary S.; Taffe, Bonita G.; Boesch, Robert; Selikoff, Irving J.

doi:10.1016/0045-6535(82)90204-1

Cited by 11 publications

(6 citation statements)

References 7 publications

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“…Fluoranthene is a product of incomplete combustion and has been identified in air, water, and charbroiled food (23). Due to its high hydrophobicity, fluoranthene is likely to absorb to hydrophobic materials, for example poly(dimethylsiloxane) (PDMS) (24, 25), polyacrylate (26), or octadecyl‐modified silica disk (C18 disks) (27), and fluoranthene was detected in the lipid collected from forehead skin of roofing workers (28). In the μCCA system, the majority of fluoranthene was found in the fat chamber (Figure 4).…”

Section: Discussionmentioning

confidence: 99%

Incorporation of 3T3‐L1 Cells To Mimic Bioaccumulation in a Microscale Cell Culture Analog Device for Toxicity Studies

Viravaidya

Shuler

2004

Biotechnology Progress

175

114

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Deficiencies in the early ADMET (absorption, distribution, metabolism, elimination, and toxicity) information on drug candidates extract a significant economic penalty on pharmaceutical firms. We have developed a microscale cell culture analog (microCCA) device that can potentially provide better, faster, and more efficient prediction of human and animal responses to a wide range of chemicals. The system described in this paper is a simple four-chamber microCCA ("lung"-"liver"-"fat"-"other tissue") designed on the basis of a physiologically based pharmacokinetics (PBPK) model of a rat. Cultures of L2, HepG2/C3A, and differentiated 3T3-L1 adipocytes were selected to mimic the key functions of the lung, liver, and fat compartments, respectively. Here, we have demonstrated the application of the microCCA system to study bioaccumulation, distribution, and toxicity of selected compounds. Results from the bioaccumulation study reveal that hydrophobic compounds such as fluoranthene preferentially accumulated in the fat chamber. Only a small amount of fluoranthene was observed in the liver and lung chambers. In addition, the presence of the differentiated 3T3-L1 adipocytes in the microCCA device significantly reduced naphthalene and naphthoquinone-induced glutathione (GSH) depletion. These findings suggest the potential utilization of the microCCA system to assess ADMET characteristics of the compound of interest prior to animal or human trials.

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Section: Discussionmentioning

confidence: 99%

Incorporation of 3T3‐L1 Cells To Mimic Bioaccumulation in a Microscale Cell Culture Analog Device for Toxicity Studies

Viravaidya

Shuler

2004

Biotechnology Progress

175

114

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“…The hexane was allowed to evaporate in the hood, and the residue was weighed. Weighings were performed with a Cahn 16 microbalance, accurate to ±0.1 jug. The remaining extract (0.5 mL) was chromatographed on Florisil (1.8 g in a disposable 5-mL pipette, topped with 0.5 cm of sodium sulfate, activated overnight at 130 °C).…”

Section: Methodsmentioning

confidence: 99%

Analysis of skin lipids for halogenated hydrocarbons

Wolff¹

1984

Anal. Chem.

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“…Absorption through the lungs has traditionally been considered the most important route of exposure; however, in many workplaces, absorption through skin may also be an important route of exposure. This is demonstrated in therapeutic as well as occupational exposure cases (9)(10)(11)(12). VanRooij et al (13) have demonstrated as high as 74 ng/cm2 of pyrene and 55 ng/cm2 of B[a]P on the skin of cokery workers.…”

Section: Introductionmentioning

confidence: 96%

Exposure to PAH compounds among cokery workers in the oil shale industry.

Kuljukka

Vaaranrinta

Veidebaum

et al. 1996

Environ Health Perspect

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were 5.7 pg/M3 and 8.1 pg/M3, respectively. Based on skin wipe sample analyses, the skin contamination was also obvious. The mean value of benzo[alpyrene on the samples collected after the shift was 1.2 ng/cm2. In control samples, benzo[alpyrene was not found. The mean value of urinary 1-hydroxypyrene concentration was 6.0 nmol/mmol creatinine for the exposed workers and 0.5 nmol/mmol creatinine for the controls. This study showed the usefulness of 1 -hydroxypyrene as an indicator of internal dose of polynuclear aromatic hydrocarbons. We concluded that the cokery workers at the Kohtla-J3rve plant are exposed to high concentrations of polynuclear aromatic compounds. Environ Health Perspect 104(Suppl 3): 539-541 (1996)

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Detection of polycyclic aromatic hydrocarbons in skin oil obtained from roofing workers

Cited by 11 publications

References 7 publications

Incorporation of 3T3‐L1 Cells To Mimic Bioaccumulation in a Microscale Cell Culture Analog Device for Toxicity Studies

Incorporation of 3T3‐L1 Cells To Mimic Bioaccumulation in a Microscale Cell Culture Analog Device for Toxicity Studies

Analysis of skin lipids for halogenated hydrocarbons

Exposure to PAH compounds among cokery workers in the oil shale industry.

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