Detection of Polymyxa graminis in a barley crop in Australia

Thompson, John; Clewett, T. G.; Jennings, Raeleen; Sheedy, J. G.; Owen, Kirsty; Persley, D. M.

doi:10.1007/s13313-010-0015-9

Cited by 11 publications

(10 citation statements)

References 37 publications

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“…In this study two new hosts (triticale and rye) were identified for ribotype I and this is the first identification of type I and II P. graminis sequences from the USA. Previously known hosts for ribotype II were wheat, barley, oats and rye and it had been reported in the UK, France, Belgium, Canada, Germany and Australia (Ward and Adams, ; Subr et al ., ; Vaïanopoulos et al ., ; Thompson et al ., ). This work demonstrated that it is also present in the USA and that it can infect triticale.…”

Section: Resultsmentioning

confidence: 97%

Ribosomal DNA analyses reveal greater sequence variation in Polymyxa species than previously thought and indicate the possibility of new ribotype‐host‐virus associations

Smith

Adams

Ward

2013

Environ Microbiol Rep

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Polymyxa species transmit viruses to many important crops. They are poorly understood obligate parasites occupying a distinct position in the Tree of Life. To better understand the potential for spread of Polymyxa-vectored diseases, ribosomal DNA was analysed from isolates covering a wide range of geographical locations, virus associations and hosts. Internal transcribed spacer 2 structure analysis indicated that Polymyxa graminis isolates could represent many species and there was more sequence variation within the known subgroups (ribotypes) than previously described. In cereal crops and soils from temperate climates Polymyxa isolates were usually ribotype I or II, but their host specificities or preferences were unclear. For the first time, there was evidence that ribotype I (in addition to ribotype II) could transmit SBWMV/SBCMV. Different ribotypes often occurred together in the same soil or plant. New hosts were identified for particular ribotypes, including the first detection of the sugar beet-infecting Polymyxa betae, in wheat. Unexpectedly, ribotype III-like sequences, usually restricted to crops in the tropics, were found in wheat from the USA. P. betae isolates showed limited variation (≤ 2%) and the recent change in susceptibility of sugar beet varieties to BNYVV in the USA is unlikely to be due to changes in P. betae.

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Section: Resultsmentioning

confidence: 97%

Ribosomal DNA analyses reveal greater sequence variation in Polymyxa species than previously thought and indicate the possibility of new ribotype‐host‐virus associations

Smith

Adams

Ward

2013

Environ Microbiol Rep

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“…In addition, eriophyid mites spread members of Rymovirus and Tritimovirus ( Abacarus Keifer , Aceria Keifer) [39,40,41] and whiteflies ( Bemicia Quaintance et Baker) spread members of Ipomovirus [42,43,44]. Only members of Bymovirus are transmitted by plasmodiophoroid fungus ( Polymyxa graminis Ledingham, Plasmodiophoridae ) [45,46,47,48]. It has been established that potyviruses are generally transmitted by non-circulative manner, which means that virus articles do not cross the vector cell membranes.…”

Section: Introductionmentioning

confidence: 99%

Sharka: The Past, The Present and The Future

Sochor

Babula

Adam

et al. 2012

Viruses

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Members the Potyviridae family belong to a group of plant viruses that are causing devastating plant diseases with a significant impact on agronomy and economics. Plum pox virus (PPV), as a causative agent of sharka disease, is widely discussed. The understanding of the molecular biology of potyviruses including PPV and the function of individual proteins as products of genome expression are quite necessary for the proposal the new antiviral strategies. This review brings to view the members of Potyviridae family with respect to plum pox virus. The genome of potyviruses is discussed with respect to protein products of its expression and their function. Plum pox virus distribution, genome organization, transmission and biochemical changes in infected plants are introduced. In addition, techniques used in PPV detection are accentuated and discussed, especially with respect to new modern techniques of nucleic acids isolation, based on the nanotechnological approach. Finally, perspectives on the future of possibilities for nanotechnology application in PPV determination/identification are outlined.

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“…tepida according to polymerase chain reaction (PCR) results (33). tepida according to polymerase chain reaction (PCR) results (33).…”

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confidence: 99%

Polymyxa graminis Isolates from Australia: Identification in Wheat Roots and Soil, Molecular Characterization, and Wide Genetic Diversity

2014

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A ustralia; H. Luo, C rop Protection Branch, D epartm ent o f A griculture and Food W est ern A ustralia, Perth, WA 6983, A ustralia; and R. A. C. Jones, Crop Protection Branch, D epartm ent of A griculture and Food Western A ustralia, Abstract Cox, B. A., Luo, H., and Jones, R. A. C. 2014. Polymyxa graminis isolates from Australia: Identification in wheat roots and soil, molecular charac terization, and wide genetic diversity. Plant Dis. 98:1567-1575.Polymyxa graminis is an obligate parasite of roots and an important vector of viruses that damage cereal crops in different parts of the world. In 2011 and 2012, P. graminis was identified infecting 11 wheat root samples from three widely dispersed locations in southwest Aus tralia. Its presence was detected by polymerase chain reaction (PCR) and confirmed by DNA sequencing of the transcribed regions of its ribosomal RNA genes (rDNA) and observing sporosori of character istic morphology and size in stained wheat roots. Also, when soil sam ples were collected from two locations where P. graminis was found and wheat bait plants grown in them, P. graminis was detected in their roots by PCR. Ribosomal DNA sequences of six southwest Australian isolates were obtained from wheat roots, and one northeast Australian isolate from barley roots. When these seven P. graminis sequences were compared with others from GenBank by phylogenetic analysis, three southwest Australian isolates were classified as P graminis f. sp. temperata (ribotypes la and lb), and three as f. sp. tepida (ribotypes Ila and lib). P graminis f. sp. temperata and tepida both occur in Corresponding author: R. A. C. Jones,

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Detection of Polymyxa graminis in a barley crop in Australia

Cited by 11 publications

References 37 publications

Ribosomal DNA analyses reveal greater sequence variation in Polymyxa species than previously thought and indicate the possibility of new ribotype‐host‐virus associations

Ribosomal DNA analyses reveal greater sequence variation in Polymyxa species than previously thought and indicate the possibility of new ribotype‐host‐virus associations

Sharka: The Past, The Present and The Future

Polymyxa graminis Isolates from Australia: Identification in Wheat Roots and Soil, Molecular Characterization, and Wide Genetic Diversity

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