Detection of potato mop-top virus in soils and potato tubers using bait-plant bioassay, ELISA and RT-PCR

Arif, Muhammad; Ali, Murad; Rehman, Anayatur; Fahim, Muhammad

doi:10.1016/j.jviromet.2013.10.022

Cited by 16 publications

(11 citation statements)

References 37 publications

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“…Potato mop top virus (PMTV) cannot manage easily because no resistant potato cultivars are available, which would be completely immune to S. subterranean (Sss) (Arif et al, 2014). The potatoes once infected by viruses, they will remain infected for the remainder of their lives.…”

Section: Management Of Pmtvmentioning

confidence: 99%

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A Review Paper on Potato Mop-Top Virus (Pmtv): Occurrence, Properties and Management

Abbas

Madadi

2016

World J. Biol. Biotechnol.

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Potato mop-top virus (PMTV) is a plant pathogenic virus that affects potatoes. The virus was initially reported from Germany but now it has spread throughout Europe, Asia, South America and North America. It is responsible for spraing symptoms (brown arcs/lines, blemishes, and rings) on potato tubers and yellow chevrons or mopping (Shortened internodes) in the leaves and stems of plants grown from infected potato tubers. PMTV causes huge economic losses due to poor tuber quality. It is an important disease in the potato growing areas of the world. PMTV is tubular rod shape and has a single stranded positive sense RNA (+ssRNA) tripartite genome. RNA 1 encodes RdRp (viral RNA-dependent RNA polymerase). Coat protein (20kDa) and a larger protein (91kDa) is encoded by RNA2. RNA2 encodes larger protein (91 kDa) by read through (RT) of the amber termination codon of the coat protein. There are three conserved moldular sets of genes known as triple gene block (TGB) which are coded by RNA3. These TGBs are involved in cell to cell or long distance movement of PMTV. In nature, PMTV is vectored and transmitted by a soil born pathogen (Plasmodiophorid (Spongospora subterranean f.sp. subterranean abbreviated as ‘Sss’) that itself causes the powdery scab disease on tubers. The disease caused by PMTV and Sss are favored by cool and damp conditions. PMTV remain in spore balls of Sss for several years even if the potato is not grown in the field. There are no efficient means to manage the virus nor its vector in an infested field, therefore, preventive measures are essential. Since PMTV along with its vector is causing important disease of potato, so understanding its molecular, biological, physical properties and management strategies is very important.

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Section: Management Of Pmtvmentioning

confidence: 99%

“…RT-PCR along with the baiting methods to trapped Sss have proven effective in the detection of PMTV (Nakayama et al, 2010). For successful detection of PMTV from the soil and plant samples a combination of infectivity assays, ELISA and RT-PCR has recently been reported by a researcher (Arif et al, 2014).PMTV and Vector: S. subterraneaf. sp.…”

mentioning

confidence: 99%

A Review Paper on Potato Mop-Top Virus (Pmtv): Occurrence, Properties and Management

Abbas

Madadi

2016

World J. Biol. Biotechnol.

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“…Only a limited number of samples can be tested in one run and it is the main reason that this technique is limited to scientific labs rather than plant diagnostic labs. Rapid response and high sensitivity enable PCR a more reliable method for testing the limited number of 'mother' seed stocks/plants (Arif et al, 2014). RT-PCR was optimized for Alfalfa mosaic virus (AMV), Impatiens necrotic spot virus (INSV), Tobacco rattle virus (TRV), Tomato spotted wilt virus (TSWV), PLRV, PMTV, PVA, PVM, PVS, PVX, PVY and Potato spindle tuber viroid (PSTVd).…”

Section: Immunosorbent Electron Microscopy (Isem)mentioning

confidence: 99%

Different Techniques for Diagnostic of Potato Viruses: A Brief Review

Qamar

Batool

Aurangzeb

et al. 2016

World J. Biol. Biotechnol.

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Potato is ranked fourth among the food crops and fifth for human consumption. It provides more yield as compared to cereals and gives more calories. Fungal, viral, thyroid, bacteria, nematode, phytoplasmas and abiotic factors play a pivotal role in yield reduction of potato crop. 38 different potato viruses naturally infect potato crops and PVA, PVM, PVS, PVX, PVY, PLRV and PMTV are reported in three consecutive potato crop of Pakistan. Increasing incidence of PVX and PVY in main potato growing areas of is getting an alarming position and PLRV has caused significant yield losses. The present review article demonstrates different techniques for identification and detection of these viruses..

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“…The plants were uprooted after eight weeks for virus testing through back inoculation of sap from roots and leaves to indicator plants (Chenopodium quinoa, Chenopodium amaranticolor, B. vulgaris cv Kewiterma, Spinacea oleracea, Tetragonia expansa and Nicotiana benthamiana). Both root and leaves samples of baited B. vulgaris were also tested by double antibody sandwich-enzyme-linked immunosorbent assay (DAS-ELISA) (Koenig et al, 1997;Arif et al, 2014) and by reverse transcription polymerase chain reaction (RT-PCR) (EPPO, 2004)…”

Section: Soil-bait Testmentioning

confidence: 99%

“…DAS-ELISA was done in root and leaf samples of bait plants for the detection of BNYVV as described by Koenig et al (1997) and Arif et al (2014). The tests were performed in polystyrene micro-plates (NUNC, Immunoplate II, Thermal Scientific, USA) following manufacturer's instructions.…”

Section: Serological Detection Of Bnyvv In Bait Plant Through Das-elisamentioning

confidence: 99%

Detection of beet necrotic yellow vein virus in Pakistan using bait-plant bioassay, ELISA and RT-PCR

Arif¹,

Khan²,

Shafi³

et al. 2015

Afr. J. Biotechnol.

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The Northwestern plains of Pakistan are the major sugar beet producing region in the country, providing an important alternative to sugar cane for sugar production when sugar cane is absent in the fields. We surveyed this region for four consecutive years and found that Beet necrotic yellow vein virus (BNYVV) is prevalent in at least five of these districts (Peshawar, Charsadda, Nowshera, Mardan and Swabi). An increase in virus incidence was observed in 2012 as compared to previous years (2009 to 2011) in all the sugar beet growing districts surveyed. The identity of the virus was confirmed using bait bioassay, enzyme-linked immunosorbent assay (ELISA), reverse transcription-polymerase chain reaction (RT-PCR) and infectivity assay in roots and leaves of bait plants and sugar beet commercial cultivars. The results indicate that the virus was detected in at least 17 (out of 20) locations and all the four sugar beet cultivars commercially grown in the region were found susceptible to the virus. Our results indicate that bait plant bioassay, ELISA, RT-PCR and infectivity assay can efficiently detect BNYVV in roots and leaves of baited plants, field samples and sugar beet cultivars commercially grown in the region. This is the first report of BNYVV in Pakistan using both conventional and molecular techniques.

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Detection of potato mop-top virus in soils and potato tubers using bait-plant bioassay, ELISA and RT-PCR

Cited by 16 publications

References 37 publications

A Review Paper on Potato Mop-Top Virus (Pmtv): Occurrence, Properties and Management

A Review Paper on Potato Mop-Top Virus (Pmtv): Occurrence, Properties and Management

Different Techniques for Diagnostic of Potato Viruses: A Brief Review

Detection of beet necrotic yellow vein virus in Pakistan using bait-plant bioassay, ELISA and RT-PCR

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