Detection of potential osteoarthritis biomarkers using surface enhanced Raman spectroscopy in the near-infrared

Mandair, Gurjit S.; Dehring, Karen A.; Roessler, Blake J.; Morris, Michael D.

doi:10.1117/12.644790

Cited by 4 publications

(3 citation statements)

References 17 publications

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“…As an important component of SF, HA has attracted the attention of researchers. Mandair et al [ 116 ] found that SERS could greatly enhance the signal intensity of HA, such that the minimum detectable concentration could be as low as 0.5 mg/mL. At the same time, effective protein removal techniques, including the trichloroacetic acid precipitation method, centrifugal method, and droplet deposition method, can effectively remove the influence of protein on Raman spectrum to observe HA more clearly.…”

Section: Raman Spectroscopymentioning

confidence: 99%

Vibrational Spectroscopy in Assessment of Early Osteoarthritis—A Narrative Review

Chen

Zhao

et al. 2021

IJMS

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Osteoarthritis (OA) is a degenerative disease, and there is currently no effective medicine to cure it. Early prevention and treatment can effectively reduce the pain of OA patients and save costs. Therefore, it is necessary to diagnose OA at an early stage. There are various diagnostic methods for OA, but the methods applied to early diagnosis are limited. Ordinary optical diagnosis is confined to the surface, while laboratory tests, such as rheumatoid factor inspection and physical arthritis checks, are too trivial or time-consuming. Evidently, there is an urgent need to develop a rapid nondestructive detection method for the early diagnosis of OA. Vibrational spectroscopy is a rapid and nondestructive technique that has attracted much attention. In this review, near-infrared (NIR), infrared, (IR) and Raman spectroscopy were introduced to show their potential in early OA diagnosis. The basic principles were discussed first, and then the research progress to date was discussed, as well as its limitations and the direction of development. Finally, all methods were compared, and vibrational spectroscopy was demonstrated that it could be used as a promising tool for early OA diagnosis. This review provides theoretical support for the application and development of vibrational spectroscopy technology in OA diagnosis, providing a new strategy for the nondestructive and rapid diagnosis of arthritis and promoting the development and clinical application of a component-based molecular spectrum detection technology.

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Section: Raman Spectroscopymentioning

confidence: 99%

Vibrational Spectroscopy in Assessment of Early Osteoarthritis—A Narrative Review

Chen

Zhao

et al. 2021

IJMS

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“…Quantitative chemical methods described in the literature to measure HA concentration include normal phase, reverse-phase ion-pair, and anion-exchange chromatography utilizing ultraviolet absorption and chemiluminescence, 17 high-performance liquid chromatography (HPLC) with fluorometric detection, 17 and surface-enhanced Raman spectroscopy. 18 , 19 Qualitative methods to image HA in tissues or on surfaces are primarily microscopy-based, notably optical and confocal microscopies. 20 – 22 Because HA is a multinegatively charged biopolymer, it binds cationic dyes, among them alcian blue (AB) 22 , 23 and Stains-All (E9379; Sigma-Aldrich Co, St Louis, MO, USA).…”

Section: Introductionmentioning

confidence: 99%

Visualization of a hyaluronan network on the surface of silicone-hydrogel materials

Wyglądacz¹,

Hook²

2016

OPTH

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Biotrue multipurpose solution (MPS) is a bioinspired disinfecting and conditioning solution that includes hyaluronic acid (HA) as a natural wetting agent. Previous studies demonstrated that HA sorbed from Biotrue MPS on both conventional and silicone hydrogel (SiHy) contact lens materials; an in vitro simulated-wear test validated the presence of HA on the lens surfaces for as long as 20 hours. In this study, the morphology and distribution of HA sorbed from both Biotrue and pure HA solution on SiHy contact lens surfaces was examined. Atomic force microscopy imaging was used to illustrate the topography of fresh SiHy contact lens materials before and after incubation with 0.1% (w/v) HA solution. The distribution, as well as fine details of the HA network, were resolved by first staining HA with Gram’s safranin, then imaging with confocal laser-scanning microscopy and differential interference-contrast microscopy. In this approach, SiHy materials take up the dye (safranin) nonspecifically, such that the resultant safranin–HA complex appears dim against the fluorescent lens background. Balafilcon A was chosen as the representative of glassy SiHy lenses that require postpolymerization plasma treatment to increase wettability. Senofilcon A and samfilcon A were chosen as representatives of SiHy materials fabricated with an internal wetting agent. A confluent and dim HA–safranin network was observed adhered to balafilcon A, senofilcon A, and samfilcon A lens surfaces incubated with either 0.1% (w/v) HA solution or Biotrue MPS. Therefore, the conditioning function provided by Biotrue MPS may be in part explained by the presence of the HA humectant layer that readily sorbs on the various types of SiHy contact lens materials.

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“…32,33 We demonstrated that SERS could detect HA drops at clinically relevant concentrations (0.125-3 mg/ml) that were undetected when spotted onto fused silica or non-enhancing gold surfaces. 34,35 Early experiments indicated that both drop shape and SERS spectra taken of the droplet were dependent upon HA concentration. 34 In this study, we examined the combined effects of drop thickness and shape, and polymer conformation on SERS spectra of hyaluronic acid drops dried onto a SERS-active surface.…”

Section: Introductionmentioning

confidence: 99%

Effect of Conformation and Drop Properties on Surface-Enhanced Raman Spectroscopy of Dried Biopolymer Drops

et al. 2008

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Biofluids are complex solutions consisting of small ions and large biopolymers such as DNA, proteins, or proteoglycans. Biopolymers affect fluid properties but their effect on drop deposition has not been examined. Hyaluronic acid (HA), an important component in synovial fluid, was chosen as a model biopolymer, and examined using surface-enhanced Raman spectroscopy (SERS). Nanoliter volumes of HA solutions were dried onto a patterned SERS substrate and spectra were collected from the dried hyaluronic acid drops with a near-infrared Raman microscope. Characteristic hyaluronic acid bands were examined. Capillary viscometry measured properties of HA solutions, and entanglement behavior was also modeled using scaling theory principles. Viscosity measurements were incorporated into models of suspended particle droplets to account for the effect of inter-chain attraction on droplet formation. Microscope images were used to evaluate the shape of the dried drop. Relative drop thickness was estimated from concentric rings found at drop edges using established models of light interference by thin films. We found SERS spectra were sensitive not only to polymer conformation, but also to type of deposition (ring versus uniform), and the thickness of the resulting deposition. These data suggest an approach to elucidate the effects of biopolymers and dehydrated biofluids on SERS analysis.

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Detection of potential osteoarthritis biomarkers using surface enhanced Raman spectroscopy in the near-infrared

Cited by 4 publications

References 17 publications

Vibrational Spectroscopy in Assessment of Early Osteoarthritis—A Narrative Review

Vibrational Spectroscopy in Assessment of Early Osteoarthritis—A Narrative Review

Visualization of a hyaluronan network on the surface of silicone-hydrogel materials

Effect of Conformation and Drop Properties on Surface-Enhanced Raman Spectroscopy of Dried Biopolymer Drops

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