Detection of potyviruses infecting Iranian common beans using broad-spectrum antibodies and universal primer pairs

Genetic diversity and evolutionary analyses of potyviruses infecting narcissus in Iran

“…Moreover, potyvirus infections of some ELISA positive samples with good quantities and qualities of RNA extracts failed to be detected by the primers used. This may be due to the presence of nucleotide variants in the genome of these isolates that are not complementary to the primer sequences, or that the potyvirus antibodies non-specifically react with other viruses (Valouzi et al 2019 ). This study also confirmed the capacity of NIb and Tu3T9M universal primers to amplify Iranian potyvirus genomes (Golnaraghi et al 2015 ; Valouzi et al 2017 , 2019 ).…”

“…This may be due to the presence of nucleotide variants in the genome of these isolates that are not complementary to the primer sequences, or that the potyvirus antibodies non-specifically react with other viruses (Valouzi et al 2019 ). This study also confirmed the capacity of NIb and Tu3T9M universal primers to amplify Iranian potyvirus genomes (Golnaraghi et al 2015 ; Valouzi et al 2017 , 2019 ). We found NYSV and NLSYV in Iranian field- and greenhouse-grown narcissus plants, respectively.…”