Detection of protein alterations in male breast cancer using two dimensional gel electrophoresis and mass spectrometry: The involvement of several pathways in tumorigenesis

Chahed, Karim; Kabbage, Maria; Hamrita, Bechr; Guillier, Christelle; Trimèche, Mounir; Remadi, Sami; Ehret‐Sabatier, Laurence; Chouchane, Lotfi

doi:10.1016/j.cca.2007.10.018

Cited by 25 publications

(17 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of the 26 cancer up‐regulated proteins identified in our study, only calreticulin (Franzén et al., 1997; Bini et al., 1997), lactate dehydrogenase (Franzén et al., 1997), GST‐pi (Franzén et al., 1997), thioredoxin (Bini et al., 1997), PDI (Bini et al., 1997; Chahed et al., 2008), TPI (Zhang et al., 2005), EF‐1‐beta (Deng et al., 2006), and galectin 1 (Chahed et al., 2008) have been previously described as deregulated in breast cancer, albeit in a very limited number of tumours tissue samples. All 26 markers have been identified in conditioned media of three breast cell lines: MCF‐10A (a basal B subtype with intact p53), BT474 (a luminal subtype) and MDA‐MB‐468 (a basal A‐like subtype) (Kulasingam and Diamandis, 2007), confirming these proteins as bona fide externalised proteins and emphasizing the validity of the approach we took.…”

Section: Discussionmentioning

confidence: 81%

Up‐regulated Proteins in the Fluid Bathing the Tumour Cell Microenvironment as Potential Serological Markers for Early Detection of Cancer of the Breast

et al. 2009

View full text Add to dashboard Cite

Breast cancer is by far the most common diagnosed form of cancer and the leading cause of cancer death in women today. Clinically useful biomarkers for early detection of breast cancer could lead to a significant reduction in mortality. Here we describe a detailed analysis using gel-based proteomics in combination with mass spectrometry and immunohistochemistry (IHC) of the tumour interstitial fluids (TIF) and normal interstitial fluids (NIF) collected from 69 prospective breast cancer patients. The goal of this study was to identify abundant cancer up-regulated proteins that are externalised by cells in the tumour microenvironment of most if not all these lesions. To this end, we applied a phased biomarker discovery research strategy to the analysis of these samples rather than comparing all samples among each other, with inherent inter and intra-sample variability problems. To this end, we chose to use samples derived from a single tumour/benign tissue pair (patient 46, triple negative tumour), for which we had well-matched samples in terms of epithelial cell numbers, to generate the initial dataset. In this first phase we found 110 proteins that were up-regulated by a factor of 2 or more in the TIF, some of which were confirmed by IHC. In the second phase, we carried out a systematic computer assisted analysis of the 2D gels of the remaining 68 TIF samples in order to identify TIF 46 up-regulated proteins that were deregulated in 90% or more of all the available TIFs, thus representing common breast cancer markers. This second phase singled out a set of 26 breast cancer markers, most of which were also identified by a complementary analysis using LC-MS/MS. The expression of calreticulin, cellular retinoic acid-binding protein II, chloride intracellular channel protein 1, EF-1-beta, galectin 1, peroxiredoxin-2, platelet-derived endothelial cell growth factor, protein disulfide isomerase and ubiquitin carboxyl-terminal hydrolase 5 were further validated using a tissue microarray containing 70 malignant breast carcinomas of various grades of atypia. A significant number of these proteins have already been detected in the blood/plasma/secretome by others. The next steps, which include biomarker prioritization based on the hierarchal evaluation of these markers, antibody and antigen development, assay development, analytical validation, and preliminary testing in the blood of healthy and breast cancer patients, are discussed.

show abstract

Section: Discussionmentioning

confidence: 81%

Up‐regulated Proteins in the Fluid Bathing the Tumour Cell Microenvironment as Potential Serological Markers for Early Detection of Cancer of the Breast

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Using such methods, proteins such as peroxiredoxin 3 (Ai et al, 2006; Chen et al, 2010b; Guo et al, 2007; Li et al, 2008; Lu et al, 2010; Qiao et al, 2012; Song et al, 2009; Wang, 2007; Yue et al, 2007) and osteopontin have been identified as potential markers of HCC (Abu El Makarem et al, 2011; Chen et al, 2010a; Chen et al, 2011; Kim et al, 2006; Lin et al, 2011; Phillips et al, 2012; Qin and Tang, 2004; Shang et al, 2012; Tang et al, 2004; Ye et al, 2003; Zekri et al, 2011). While these markers have been shown to be elevated in other cancers, they may still have great value in the early detection of liver cancer (Chahed et al, 2008; Lehtonen et al, 2004; McAllister et al, 2008; Mirza et al, 2008; Park et al, 2008; Park et al, 2007; Reiniger et al, 2007; Schremmer et al, 2007; Tigrani and Weydert, 2007; Tuck et al, 2007; Whiteaker et al, 2007). However, large multi-center studies using peroxiredoxin 3 and osteopontin have either not been done or have not been successful enough at detecting HCC in the background of cirrhosis to alter clinical care.…”

Section: Proteomic Identification Of Biomarkers Of Liver Cancermentioning

confidence: 99%

Glycosylation and Liver Cancer

Mehta

Herrera

Block

2015

Advances in Cancer Research

119

View full text Add to dashboard Cite

Liver cancer is the 5th most common cancer, but the 2nd leading cause of cancer death, in the world, with more than 700,000 fatalities annually. The major etiology of liver cancer is infection with an hepatotropic virus such as hepatitis B virus (HBV) or hepatitis C virus infection (HCV). While chronic viral infection remains the main cause of liver disease and risk of HCC, rates of non –viral associated HCC are occurring at an alarmingly increasing rate. Like many cancers, survival rates are closely associated with time of detection. If HCC is caught early, survival rates can be as high as 50%. Regrettably, most cases of HCC are caught late where survival rates can be as low as 2–7%. Thus, there has been great interest in discovering serum biomarkers that could be used to identify those with HCC. To this end, many groups have examined the N-linked glycans to identify changes that occur with HCC. As the liver secretes the vast majority of proteins into the serum, this has often been a starting point for study. In serum, alterations in core fucosylation, outer-arm fucosylation, increased sialylation and glycan branching have been observed in patients with HCC. Similar findings have been found directly in HCC tissue suggesting that these glycan changes may play a role in tumor formation and development.

show abstract

“…Therefore, any changes in the protein composition of virally infected cells could be detected easily using this technique. In fact, this technique was used lately for the detection and identification of protein alterations in cancer cells,9, 10 human viruses,11–13 and microorganisms 14…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic detection and identification of infected cells with herpes viruses

2008

View full text Add to dashboard Cite

Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and Fourier transform infrared (FTIR) microspectroscopy were previously applied for the identification of various biological samples. In the present study, normal cells in culture and cells infected with herpes simplex virus type 2 (HSV-2) or varicella-zoster virus (VZV) were analyzed by MALDI-TOF and FTIR microscopy. Specific spectral biomarkers for rapid and reliable monitoring and identification of infected cells and probably for the discrimination between these viruses were searched. The results show consistent spectral peaks in all examined normal uninfected human fibroblast cells both in MALDI-T0F and FTIR microscopy. In HSV-2- or VZV-infected cells, two unique peaks for each appeared at m/z 5397 and 5813 or at m/z 3501 and 4951, respectively, in MALDI-TOF spectra. In addition, several peaks that appeared in control uninfected cells at the region m/z 13,000-20,000 disappeared completely in all examined infected samples. When these infected cells were examined by FTIR microscopy, a band at 859 cm(-1) in control uninfected cells was significantly shifted to 854 cm(-1) in both HSV2- and VZV-infected cells. In addition, phosphate levels were considerably increased in all infected cells compared to normal uninfected cells. These parameters could be used as a basis for developing a spectral method for the detection and identification of cells infected with herpes viruses.

show abstract

Detection of protein alterations in male breast cancer using two dimensional gel electrophoresis and mass spectrometry: The involvement of several pathways in tumorigenesis

Cited by 25 publications

References 27 publications

Up‐regulated Proteins in the Fluid Bathing the Tumour Cell Microenvironment as Potential Serological Markers for Early Detection of Cancer of the Breast

Up‐regulated Proteins in the Fluid Bathing the Tumour Cell Microenvironment as Potential Serological Markers for Early Detection of Cancer of the Breast

Glycosylation and Liver Cancer

Spectroscopic detection and identification of infected cells with herpes viruses

Contact Info

Product

Resources

About