Detection of protein from detergent solutions by probe electrospray ionization mass spectrometry (PESI‐MS)

Mandal, Mridul Kanti; Chen, Lee Chuin; Yu, Zhan; Nonami, Hiroshi; Erra‐Balsells, Rosa; Hiraoka, Kenzo

doi:10.1002/jms.1977

Cited by 46 publications

(45 citation statements)

References 41 publications

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“…Some noncapillary tips such as stainless steel needle [6,19], paper [13,[20][21][22][23], and wooden tips [14] enabled direct analysis of raw samples. Hiraoka and co-workers reported the sequential and exhaustive ionization of analytes on solid metal probe [24].…”

Section: E Lectrospray Ionization (Esi) Is a Powerful Massmentioning

confidence: 99%

Analytical Properties of Solid-substrate Electrospray Ionization Mass Spectrometry

Yao

2012

J. Am. Soc. Mass Spectrom.

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Abstract. Conventional electrospray ionization mass spectrometry (ESI-MS) uses a capillary for sample loading and ionization. Along with the development of ambient ionization techniques, ESI-MS using noncapillary emitters has attracted more interest in recent years. Following our recent report on ESI-MS using wooden tips (Anal. Chem. 83, 8201-8207 (2011)), the technique was further investigated and extended in this study. Our results revealed that the wooden tips could serve as a chromatographic column for separation of sample components. Sequential and exhaustive ionization was observed for proteins and salts on wooden tips with salts ionized sooner and proteins later. Nonconductive materials that contain microchannels/pores could be used as tips for ESI-MS analysis with sample solutions loaded to the sharp-ends only, since rapid diffusion of sample solutions by capillary action would enable the tips to become conductive. Tips of inert materials such as bamboo, fabrics, and sponge could be used for sample loading and ionization, while samples such as tissue, mushroom, and bone could form tips to induce ionization for direct analysis with application of a high voltage.

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Section: E Lectrospray Ionization (Esi) Is a Powerful Massmentioning

confidence: 99%

Analytical Properties of Solid-substrate Electrospray Ionization Mass Spectrometry

Yao

2012

J. Am. Soc. Mass Spectrom.

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“…20,43 In this situation, it becomes necessary for us to perform such a study in order to accurately assess the performance of our FFZE device toward enabling direct ESI-MS analysis of cationic analytes prepared in SDS matrices. To this end, human angiotensin I samples prepared in a solvent containing 25% methanol (v/v) in deionized water and various amounts of SDS were analyzed using the ESI-MS technique.…”

Section: Resultsmentioning

confidence: 99%

Electrophoretic Extraction of Low Molecular Weight Cationic Analytes from Sodium Dodecyl Sulfate Containing Sample Matrices for Their Direct Electrospray Ionization Mass Spectrometry

2015

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While the use of sodium dodecyl sulfate (SDS) in separation buffers allows efficient analysis of complex mixtures, its presence in the sample matrix is known to severely interfere with the mass-spectrometric characterization of analyte molecules. In this article, we report a microfluidic device that addresses this analytical challenge by enabling inline electrospray ionization mass spectrometry (ESI-MS) of low molecular weight cationic samples prepared in SDS containing matrices. The functionality of this device relies on the continuous extraction of analyte molecules into an SDS-free solvent stream based on the free-flow zone electrophoresis (FFZE) technique prior to their ESI-MS analysis. The reported extraction was accomplished in our current work in a glass channel with microelectrodes fabricated along its sidewalls to realize the desired electric field. Our experiments show that a key challenge to successfully operating such a device is to suppress the electroosmotically driven fluid circulations generated in its extraction channel that otherwise tend to vigorously mix the liquid streams flowing through this duct. A new coating medium, N-(2-triethoxysilylpropyl) formamide, recently demonstrated by our laboratory to nearly eliminate electroosmotic flow in glass microchannels was employed to address this issue. Applying this surface modifier, we were able to efficiently extract two different peptides, human angiotensin I and MRFA, individually from an SDS containing matrix using the FFZE method and detect them at concentrations down to 3.7 and 6.3 µg/mL, respectively, in samples containing as much as 10 mM SDS. Notice that in addition to greatly reducing the amount of SDS entering the MS instrument, the reported approach allows rapid solvent exchange for facilitating efficient analyte ionization desired in ESI-MS analysis.

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“…One important highlight of PESI technique is that PESI is more tolerant to salts and detergents than capillarybased conventional ESI. 41,42) In addition, Hu et al demonstrated that commonly available aluminium foils could also be used to assist the generation of electrospray. 43) All the abovementioned techniques reiterate the fact that metal substrates are quite e ective in assisting for generation of electrospray in an open air setup.…”

Section: Esi Emitter Solid Metal Substratesmentioning

confidence: 99%

Electrospray Modifications for Advancing Mass Spectrometric Analysis

Meher

Chen

2017

Mass Spectrometry

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Generation of analyte ions in gas phase is a primary requirement for mass spectrometric analysis. One of the ionization techniques that can be used to generate gas phase ions is electrospray ionization (ESI). ESI is a so ionization method that can be used to analyze analytes ranging from small organics to large biomolecules. Numerous ionization techniques derived from ESI have been reported in the past two decades.ese ion sources are aimed to achieve simplicity and ease of operation. Many of these ionization methods allow the exibility for elimination or minimization of sample preparation steps prior to mass spectrometric analysis. Such ion sources have opened up new possibilities for taking scienti c challenges, which might be limited by the conventional ESI technique. us, the number of ESI variants continues to increase. is review provides an overview of ionization techniques based on the use of electrospray reported in recent years. Also, a brief discussion on the instrumentation, underlying processes, and selected applications is also presented.

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Detection of protein from detergent solutions by probe electrospray ionization mass spectrometry (PESI‐MS)

Cited by 46 publications

References 41 publications

Analytical Properties of Solid-substrate Electrospray Ionization Mass Spectrometry

Analytical Properties of Solid-substrate Electrospray Ionization Mass Spectrometry

Electrophoretic Extraction of Low Molecular Weight Cationic Analytes from Sodium Dodecyl Sulfate Containing Sample Matrices for Their Direct Electrospray Ionization Mass Spectrometry

Electrospray Modifications for Advancing Mass Spectrometric Analysis

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