Detection of Protein Uptake in In Vitro Cultured Astrocytes Exemplified by the Uptake of the Serine Protease, Tissue Plasminogen Activator

Bres, Ewa E.; Faissner, Andréas

doi:10.1007/978-1-4939-9068-9_14

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“…Therefore, a deletion of Lrp1 from the astrocyte membrane should reduce the clearance of tPA at active synapses. When the Lrp1-deficient astrocytes were tested the ability for tPA uptake was found strongly reduced ( Bres and Faissner, 2019a ; Bres et al, 2020 ). This led to the concept that the Emx1-Cre::Lrp1 fl/fl mutant is characterized by an elevation of NMDARs, reduced clearance of tPA and transiently compromised glutamate clearance that result in a disturbed tPA-NMDAR signaling axis.…”

Section: Introductionmentioning

confidence: 99%

Low-density lipoprotein receptor-related protein-1 (LRP1) in the glial lineage modulates neuronal excitability

Faissner

2023

Front. Netw. Physiol.

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The low-density lipoprotein related protein receptor 1 (LRP1), also known as CD91 or α-Macroglobulin-receptor, is a transmembrane receptor that interacts with more than 40 known ligands. It plays an important biological role as receptor of morphogens, extracellular matrix molecules, cytokines, proteases, protease inhibitors and pathogens. In the CNS, it has primarily been studied as a receptor and clearance agent of pathogenic factors such as Aβ-peptide and, lately, Tau protein that is relevant for tissue homeostasis and protection against neurodegenerative processes. Recently, it was found that LRP1 expresses the Lewis-X (Lex) carbohydrate motif and is expressed in the neural stem cell compartment. The removal of Lrp1 from the cortical radial glia compartment generates a strong phenotype with severe motor deficits, seizures and a reduced life span. The present review discusses approaches that have been taken to address the neurodevelopmental significance of LRP1 by creating novel, lineage-specific constitutive or conditional knockout mouse lines. Deficits in the stem cell compartment may be at the root of severe CNS pathologies.

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Section: Introductionmentioning

confidence: 99%