Detection of proteins and bacteria using an array of feedback capacitance sensors

Mehta, Manav; Hanumanthaiah, Chandra Sekar; Betala, Pravin A.; Zhang, Hong; Roh, Sae-Weon; Buttner, William J.; Penrose, W.R.; Stetter, Joseph R.; Pérez-Luna, Víctor H.

doi:10.1016/j.bios.2007.08.011

Cited by 10 publications

(5 citation statements)

References 36 publications

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“…2, the proposed sensing scheme consists of the following components: a single current-source (Ibias), differential pairs of working electrodes (El and E2), a common voltage (Vcom) for the DC potential level shift, and a switch controller with four phases ((/JJ , (h, (/J3, and (M· The sensor replaces the double current-sources with a combination of the single current-source and the switch operation. Therefore, the single current-source clearly overcomes the mismatch between the charging and discharging current flows, unlike the bidirectional current excitation used in earlier studies [9][10][11]. The differential architecture in this device detects the relative difference in the property between a bare probe-functionalized working electrode (El) and a hybridized working electrode (E2)' Each working electrodes (EI and E2) has a size of 1.S mm x 1.7 mm.…”

Section: Operating Principlementioning

confidence: 96%

“…Gr,water is 81, whereas Gr,mo/eco/e is 2 or 3). This paper focuses on the capacitance measurement technology using the current excitation [9][10][11]. The current charges or discharges the capacitor at the electrolyte-electrode interface while changing the electrode voltage.…”

Section: Introductionmentioning

confidence: 99%

“…This method can estimate the capacitance with a low complexity compared with other capacitive sensors [4,[12][13][14]. However, there are several issues that degrade the sensor sensitivity, such as the DC drift in the electrode potential that is caused by a mismatch between the excitation currents [9], the offset in the extracted capacitance of interest due to the electrode voltage charged initially [9,10], and the weakness against common-mode noises, such as power-supply noise or substrate coupling noise [8][9][10][11]. These issues may cause large standard deviations and poor reproducibility of the measured signal, thereby limiting the performance of the sensor.…”

Section: Introductionmentioning

confidence: 99%

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An alternating current-driven control for biomolecule detection

Lee

2015

2015 15th International Conference on Control, Automation and Systems (ICCAS)

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A biosensor with an alternating current-driven control is realized for label-free detection of biomolecule. This sensor precisely measures the capacitive changes in the electrode-electrolyte interface after hybridization with complementary DNA targets. Conventional capacitive biosensors generally use the excitation method with double current-sources. However these sensors have limitations due to performance-based issues of the DC potential drift and offsets, as well as weakness against common-mode noises. In this paper, the proposed device includes differential electrode pairs that are located in a single current-source line with sensing circuits, which results in the elimination of the effect due to the current mismatch. The device also shows an improved signal-to-noise ratio. Additionally, the individual control of the switch controller in this device offers robust and reproducible measurement capabilities independent of the duty asymmetry and pre-charged initial offset voltage. The device is a hybrid sensor that has both working electrodes and readout circuitry in one-chip using a CMOS process. The prototype successfully demonstrated the specific detection of oligonucleotide sequences. The device consumes 2.34mW from a 3.3 V supply in a 0.35 I-lm CMOS process.

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Section: Operating Principlementioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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An alternating current-driven control for biomolecule detection

Lee

2015

2015 15th International Conference on Control, Automation and Systems (ICCAS)

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“…Recently, bioaerosols detection chips based on microelectro-mechanical systems (MEMS) have been studied for the development of small, portable, inexpensive and rapid detectors (Janssen, IJzendoorn, & Prins, 2008;Mehta et al, 2007). However, these detection chips require bioaerosols to be concentrated in the range of 4-13 #/cm 3 for the sampling of airborne particles (Mehta et al, 2007;Owen, Al-Kaysi, Bardeen, & Cheng, 2007).…”

Section: Introductionmentioning

confidence: 99%

New bio-aerosol collector using a micromachined virtual impactor

Park

Kim

Park

et al. 2009

Journal of Aerosol Science

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“…[1][2][3] Numerous methods for the detection of microbes have been developed and used in the field of environmental sanitation. [4][5][6][7][8][9] These detection and identification methods employ interactions with specific peptides, sugar chains, and proteins on the surface of cells. The binding of a siderophore to its receptor protein in the cell membrane is an example of such an interaction.…”

Section: Introductionmentioning

confidence: 99%

Adsorption and detection of Escherichia coli using an Au substrate modified with a catecholate-type artificial siderophore–Fe3+ complex

et al. 2013

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A catecholate-type artificial siderophore with a terminal-NH2 group (1) and its Fe(3+) complex (2) were prepared. Siderophore 1 was characterized by (1)H NMR, FT-IR, and ESI-TOF MS spectroscopy. The corresponding Fe(3+) complex 2 was obtained by reaction of 1 with Fe(acac)3. The absorption band at 500 nm (ε = 4670 M(-1) cm(-1) at pH 7.0) of the electronic absorption spectrum of 2 is assignable as the LMCT (O(catecholate) → Fe(3+)) absorption band. This band indicates the formation of the Fe(3+) complex of 1. The biological activity of 2 with respect to Escherichia coli was clearly confirmed by observing that it permeates into the cell membrane. The self-assembled monolayer of 2 on an Au substrate, 2/Au, was prepared and its preparation was confirmed by FT-IR reflection-absorption spectroscopy (IR-RAS) and cyclic voltammetry (CV). Furthermore, a quartz crystal microbalance (QCM) chip modified with 2 effectively adsorbed E. coli. M. flavescens, an organism which is incapable of synthesizing siderophores and must therefore use exogenous hydroxamate-type siderophores for growth, did not adsorb on 2/Au. In contrast, E. coli did not adsorb on the hydroxamate-type artificial siderophore-Fe(3+) complex (3)-modified Au substrate, 3/Au. These results provide preliminary evidence that microbes recognized Fe(3+) ion-bound siderophores on the surface. The detection limit of 2/Au was ∼10(4) CFU mL(-1).

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Detection of proteins and bacteria using an array of feedback capacitance sensors

Cited by 10 publications

References 36 publications

An alternating current-driven control for biomolecule detection

An alternating current-driven control for biomolecule detection

New bio-aerosol collector using a micromachined virtual impactor

Adsorption and detection of Escherichia coli using an Au substrate modified with a catecholate-type artificial siderophore–Fe3+ complex

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