Detection of Proteins Using Nano Magnetic Particle Accumulation-Based Signal Amplification

İçöz, Kutay; Mzava, Omary

doi:10.3390/app6120394

Cited by 9 publications

(11 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Method A: Adsorption and covalent bonding are widely used techniques to immobilize receptors on surfaces. Adsorption is practical and has been demonstrated for various sensors . The adsorption mechanism of protein G on gold surface was analyzed by Johnson and Mutharasan ; in our work, the solution of protein G at pH of 7.4 caused an average frequency shift of 10.7 Hz due to the adsorption on the bare gold surface (Figure ).…”

Section: Resultsmentioning

confidence: 60%

“…Antibodies recognize the analyte based on affinity and this recognition is transduced to another type of signal that can be measured . Advancements in micro and nano technology revealed development of novel transducers such as cantilevers , nanowires , surface plasmon resonators (SPR) , microelectrodes and micro/nano particles such as gold , silver nanoparticles, and immunomagnetic beads . Various methods have been reported for immobilizing antibodies on transducers or micro/nano particles .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Quartz‐crystal Microbalance Measurements of CD19 Antibody Immobilization on Gold Surface and Capturing B Lymphoblast Cells: Effect of Surface Functionalization

et al. 2018

View full text Add to dashboard Cite

We have investigated different surface functionalization methods to immobilize CD19 antibody on gold surface to capture B lymphoblast cells associated with the acute lymphoblastic leukemia disease. Quartz Crystal Microbalance measurements were performed to analyze the binding kinetics of each layer and determine the optimum method, which results in higher cell capture rates. The random orientation of antibody and oriented antibody through protein G was investigated and protein G presence resulted in 15,2 Hz frequency shift for 104 cells/mL. The 3‐mercaptopropyltrimethoxysilane (MPS) and 11‐Mercaptoundecanoic acid (MUA) coatings of gold surface together with 4‐(N‐Maleimidomethyl)cyclohexane‐1‐carboxylic acid 3‐sulfo‐N‐hydroxysuccinimide ester sodium salt (Sulfo‐SMCC) and N‐Ethyl‐N’‐(3‐dimethylaminopropyl) carbodiimide hydrochloride (EDC)/N‐hydroxysulfosuccinimide (NHS) linker layers were tested on QCM for protein G and antibody binding. The results indicate that MUA, EDC/NHS, protein G, antibody CD19 is the optimum surface modification among the tested combinations. By using the optimum surface functionalization method, minimum 103 cell per mL was measured as 1.9 Hz frequency shift.

show abstract

Section: Resultsmentioning

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Quartz‐crystal Microbalance Measurements of CD19 Antibody Immobilization on Gold Surface and Capturing B Lymphoblast Cells: Effect of Surface Functionalization

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Such amplification allows the detection of 1 ng/mL NGAL concentration in 5-fold diluted FBS, while this concentration could not be resolved by observing the fluorescent intensity of single beads. Common approaches for beads’ accumulation involve the application of magnetic forces [ 29 , 30 ] or DEP forces [ 18 ]. The increase in signal that we found to be a consequence of the beads’ accumulation is in line with what has been previously reported in literature [ 29 ].…”

Section: Resultsmentioning

confidence: 99%

Rapid Multianalyte Microfluidic Homogeneous Immunoassay on Electrokinetically Driven Beads

et al. 2020

View full text Add to dashboard Cite

The simplicity of homogeneous immunoassays makes them suitable for diagnostics of acute conditions. Indeed, the absence of washing steps reduces the binding reaction duration and favors a rapid and compact device, a critical asset for patients experiencing life-threatening diseases. In order to maximize analytical performance, standard systems employed in clinical laboratories rely largely on the use of high surface-to-volume ratio suspended moieties, such as microbeads, which provide at the same time a fast and efficient collection of analytes from the sample and controlled aggregation of collected material for improved readout. Here, we introduce an integrated microfluidic system that can perform analyte detection on antibody-decorated beads and their accumulation in confined regions within 15 min. We employed the system to the concomitant analysis of clinical concentrations of Neutrophil Gelatinase-Associated Lipocalin (NGAL) and Cystatin C in serum, two acute kidney injury (AKI) biomarkers. To this end, high-aspect-ratio, three-dimensional electrodes were integrated within a microfluidic channel to impart a controlled trajectory to antibody-decorated microbeads through the application of dielectrophoretic (DEP) forces. Beads were efficiently retained against the fluid flow of reagents, granting an efficient on-chip analyte-to-bead binding. Electrokinetic forces specific to the beads’ size were generated in the same channel, leading differently decorated beads to different readout regions of the chip. Therefore, this microfluidic multianalyte immunoassay was demonstrated as a powerful tool for the rapid detection of acute life-threatening conditions.

show abstract

“…Using these features of magnetic particles, the biosensors can be functionalized to recognize specific molecular targets, and a time and cost-efficient signal amplification can be achieved. Magnetic particles are linked to other molecular labels such as antibody to improve sensitivity and provide signal amplification for the biological measurements (İçöz and Mzava, 2016; Icoz and Savran, 2010). The sensitivity and signal amplification of biosensors can be used to address some unmet clinical needs, such as discriminating benign prostatic hypertrophy from prostate cancer via a piezeoelectric/magnetic particle-based assay (Jokerst et al, 2015).…”

Section: Magnetic Particles and Their Applicationsmentioning

confidence: 99%

Design, modeling, and control of a horizontal magnetic micromanipulator

Ablay

Böyük

İçöz

2019

Transactions of the Institute of Measurement and Control

Self Cite

View full text Add to dashboard Cite

Magnetic micromanipulators with a wide range of force generating capabilities are able to manipulate micron size particles for various applications and measurements. These magnetic particles can be coated with receptors to specifically bind to target biomolecules. In this work, a horizontal magnetic micromanipulator is designed, modeled and controlled for single micron size magnetic particle manipulations. A method is presented for dynamic modeling of magnetic micromanipulators. A feedback control method is designed that allows direct linearization of the system. It is shown that the proposed controller guarantees the stability of the closed-loop system, and yields zero steady-state error in a wide range of operation conditions. We show that the micromanipulator is able to generate a wide range of piconewton (pN) scale forces on a superparamagnetic particle for single molecule separation, and biosensor developments.

show abstract

Detection of Proteins Using Nano Magnetic Particle Accumulation-Based Signal Amplification

Cited by 9 publications

References 28 publications

Quartz‐crystal Microbalance Measurements of CD19 Antibody Immobilization on Gold Surface and Capturing B Lymphoblast Cells: Effect of Surface Functionalization

Quartz‐crystal Microbalance Measurements of CD19 Antibody Immobilization on Gold Surface and Capturing B Lymphoblast Cells: Effect of Surface Functionalization

Rapid Multianalyte Microfluidic Homogeneous Immunoassay on Electrokinetically Driven Beads

Design, modeling, and control of a horizontal magnetic micromanipulator

Contact Info

Product

Resources

About