Detection of pseudorabies virus DNA in the inner ear of intranasally infected BALB/c mice with nucleic acid hybridization in situ

Falser, N; Bandtlow, I.; Haus, M.; Wolf, Hans

doi:10.1128/jvi.57.1.335-339.1986

Cited by 15 publications

(3 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent published work and our own observations, indicate that frozen sections, fixed with acetic acidalchohol. preserve nucleic acids at the expense of cellular morphology (35). The converse appears to be true for paraformaldehyde-fixed, paraffin-embedded sections.…”

Section: In Situ Hybridizationmentioning

confidence: 98%

Tumor‐associated carbohydrate antigens

Dabelsteen

Clausen

1987

J Oral Pathology Medicine

View full text Add to dashboard Cite

Section: In Situ Hybridizationmentioning

confidence: 98%

Tumor‐associated carbohydrate antigens

Dabelsteen

Clausen

1987

J Oral Pathology Medicine

View full text Add to dashboard Cite

“…A preliminary protocol of the procedures has been described elsewhere (Gu et al, 1983;Falser et al, 1985). After an adequate cytopathic effect had been attained, neonatal hamster kidney cell culture suspensions, infected with pseudorabies virus, were spread onto slides.…”

Section: Selection Of Fixation Proceduresmentioning

confidence: 99%

“…(a) Virus inoculum: for the infection we used pseudorabies virus ATCC VR-135 strain Aujeszky from infected rabbit brain in 50% glycerol. Tissue culture methods, endpoint titration, etc., are described in detail elsewhere (Falser et al, 1985).…”

Section: Animal Experimentsmentioning

confidence: 99%

Demonstration of pseudorabies virus DNA in the mouse inner ear by an in situ nucleic acid hybridization technique in plastic embedded bony material

Falser

Bandtlow

Haus³

et al. 1986

Journal of Microscopy

View full text Add to dashboard Cite

SUMMARYThis investigation is concerned with the possibility of identifying viral DNA using the in situ DNA hybridization method in methylmethacrylate-embedded material. As an experimental model we chose viral labyrinthitis produced by intranasal infection of the mouse with pseudorabies virus. Fixation and embedding methods specially adapted to this procedure and bony histology preparation technique (specimens by grinding or micromilling) made it possible to identify viral DNA directly morphologically and virologically in the inner ear. Quantitative microphotometric analyses of trans-sagittal sections of the entire skull after in situ DNA hybridization are presented and discussed here as an explicit method of investigating the path of distribution of viral DNA in the brain and the inner ear.

show abstract

Transneuronal pathways to the vestibulocerebellum

et al. 1996

View full text Add to dashboard Cite

The alpha-herpes virus (pseudorabies, PRV) was used to observe central nervous system (CNS) pathways associated with the vestibulocerebellar system. Retrograde transneuronal migration of alpha-herpes virions from specific lobules of the gerbil and rat vestibulo-cerebellar cortex was detected immunohistochemically. Using a time series analysis, progression of infection along polyneuronal cerebellar afferent pathways was examined. Pressure injections of > 20 nanoliters of a 10(8) plaque forming units (pfu) per ml solution of virus were sufficient to initiate an infectious locus which resulted in labeled neurons in the inferior olivary subnuclei, vestibular nuclei, and their afferent cell groups in a progressive temporal fashion and in growing complexity with increasing incubation time. We show that climbing fibers and some other cerebellar afferent fibers transported the virus retrogradely from the cerebellum within 24 hours. One to three days after cerebellar infection discrete cell groups were labeled and appropriate laterality within crossed projections was preserved. Subsequent nuclei labeled with PRV after infection of the flocculus/paraflocculus, or nodulus/uvula, included the following: vestibular (e.g., z) and inferior olivary nuclei (e.g., dorsal cap), accessory oculomotor (e.g., Darkschewitsch n.) and accessory optic related nuclei, (e.g., the nucleus of the optic tract, and the medial terminal nucleus); noradrenergic, raphe, and reticular cell groups (e.g., locus coeruleus, dorsal raphe, raphe pontis, and the lateral reticular tract); other vestibulocerebellum sites, the periaqueductal gray, substantia nigra, hippocampus, thalamus and hypothalamus, amygdala, septal nuclei, and the frontal, cingulate, entorhinal, perirhinal, and insular cortices. However, there were differences in the resulting labeling between infection in either region. Double-labeling experiments revealed that vestibular efferent neurons are located adjacent to, but are not included among, flocculus-projecting supragenual neurons. PRV transport from the vestibular labyrinth and cervical muscles also resulted in CNS infections. Virus propagation in situ provides specific connectivity information based on the functional transport across synapses. The findings support and extend anatomical data regarding vestibulo-olivo-cerebellar pathways.

show abstract

Detection of pseudorabies virus DNA in the inner ear of intranasally infected BALB/c mice with nucleic acid hybridization in situ

Cited by 15 publications

References 18 publications

Tumor‐associated carbohydrate antigens

Tumor‐associated carbohydrate antigens

Demonstration of pseudorabies virus DNA in the mouse inner ear by an in situ nucleic acid hybridization technique in plastic embedded bony material

Transneuronal pathways to the vestibulocerebellum

Contact Info

Product

Resources

About