Detection of quantitative trait loci for resistance/susceptibility to pseudorabies virus in swine

Reiner, Gerald; Melchinger, Elke; Kramarova, Marcela; Pfaff, E.; Büttner, M; Saalmüller, Armin; Geldermann, H.

doi:10.1099/0022-1317-83-1-167

Cited by 55 publications

(51 citation statements)

References 23 publications

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“…The muscle load of Sarcocystis miescheriana bradyzoites after oral exposure was found to be 20 times higher in Pietrain, compared with Meishan pigs. 60 Compared with Meishan pigs, Large White pigs were found to be more susceptible to infection with pseudorabies virus 59 or K88 + Escherichia coli. 15 Compared with Duroc and Meishan pigs, Hampshire pigs developed significantly less severe macroscopic lung lesions after experimental PRRSV inoculation.…”

Section: Discussionmentioning

confidence: 99%

“…Differences in genetic susceptibility of populations or breeds to parasites, bacteria, and viruses have been reported. 9,10,30,68 Evidence exists for pig breed-dependent differences in disease resistance for a variety of pathogens including pseudorabies virus, 59 PRRSV, 27 Escherichia coli, 15,49 and Sarcocystis miescheriana. 60 Pigs of all breeds seem to be susceptible to PCV2 infection, and PMWS has been observed within a large variety of purebred and crossbred pigs submitted to a US diagnostic laboratory (P.G.…”

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Evidence of Breed-dependent Differences in Susceptibility to Porcine Circovirus Type-2-associated Disease and Lesions

et al. 2006

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Abstract. Porcine circovirus type 2 (PCV2) has been confirmed as the primary cause of postweaning multisystemic wasting syndrome (PMWS). However, in the field, PMWS is seen only in a small percentage of pigs infected with PCV2. The overall objective of the study reported here was to determine whether host genetic differences in the susceptibility to PCV2-associated disease exist among selected breeds of pigs. This study included Duroc (n 5 23), Landrace (n 5 19), and Large White (n 5 21) pigs. The pigs were infected intranasally and intramuscularly at 5-7 weeks of age with PCV2. A portion of the pigs (31/63; 30.2%) had low passively acquired PCV2 antibodies at the time of infection. There were no differences in mean weight gain, rectal temperature, or respiratory score. Clinical disease compatible with PMWS was observed only in the Landrace pigs. Most of the PCV2-infected pigs had enlarged lymph nodes, and individual Duroc and Landrace pigs had mottled tan lungs. PCV2-associated lymphoid depletion and granulomatous inflammation were observed in pigs of all breeds. Three of 19 Landrace pigs and none of the Duroc or Large White pigs developed severe lymphoid lesions associated with large amounts of intralesional PCV2 antigen typical of PMWS. Compared with seronegative Landrace pigs, Landrace pigs that had low maternal antibodies at the time of PCV2 inoculation had significantly (P , 0.05) less-severe PCV2-associated lesions. The results suggest a predisposition of the Landrace pigs of this study to PCV2-induced disease and lesions, and that low levels of passively acquired antibodies are protective.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Evidence of Breed-dependent Differences in Susceptibility to Porcine Circovirus Type-2-associated Disease and Lesions

et al. 2006

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“…However, controlled genetic studies on susceptibility to specific diseases are difficult to conduct due to size and cost limitations resulting in restrictions on pigs per line and breed. It is well known that genetic components in disease resistance exist in pigs such as reported with porcine reproductive and respiratory syndrome virus (Halbur et al, 1998;Petry et al, 2005Petry et al, , 2007Vincent et al, 2006), pseudorabies virus (Reiner et al, 2002), Salmonella sp. (Wigley, 2004), and Escherichia coli (Duchet-Suchaux et al, 1991;Michaels et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Difference in severity of porcine circovirus type two-induced pathological lesions between Landrace and Pietrain pigs1

Opriessnig

Patterson

Madson

et al. 2009

Journal of Animal Science

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Anecdotal information from the field suggests that there are host genetic differences in susceptibility to porcine circovirus type 2 (PCV2) associated disease among Landrace and Pietrain breeds. The objective of this study was to determine if a difference exists in PCV2 susceptibility between Landrace and Pi-etrain pigs under experimental conditions. Thirty-nine Landrace pigs and 39 Pietrain pigs were blocked by breed, sire, dam, and litter and randomly divided into the following 4 groups: Landrace noninoculated negative control (Landrace-NEG; n = 13), Pietrain noninoculated negative control (Pietrain-NEG; n = 13), Landrace-PCV2 (n = 26; Landrace), and Pietrain-PCV2 (n = 26; Pietrain). After waning of passively acquired anti-PCV2 antibodies, Landrace-PCV2 and Pietrain-PCV2 groups were inoculated with PCV2 isolate ISU-40895. The Landrace-NEG and Pietrain-NEG groups were housed in a separate room, remained noninoculated, and served as negative controls. All pigs in all groups were necropsied at 21 d post PCV2-inoculation. Onset of seroconversion and concentrations of anti-PCV2-IgM, anti-PCV2-IgG, and anti-PCV2 neutralizing antibodies were similar in Landrace-PCV2 and Pietrain-PCV2 groups. Furthermore, the amount of PCV2 DNA and cytokine concentrations in serum and plasma samples were not different between the 2 PCV2-inoculated groups. The severity of PCV2-associated microscopic lesions was different between Landrace and Pietrain pigs; Landrace-PCV2 pigs had significantly (P < 0.05) more severe lymphoid lesions than the Pietrain-PCV2 pigs. Although the pigs originated from the same farm where their dams were commingled, passively acquired anti-PCV2-antibodies waned in Pietrain pigs by approximately 12 wk of age, whereas the majority of the Landrace pigs remained PCV2 seropositive until 18 wk of age and beyond. The results from this study indicate that a genetic difference exists between these 2 breeds of pigs in susceptibility to PCV2-associated lesions. ABSTRACT: Anecdotal information from the field suggests that there are host genetic differences in susceptibility to porcine circovirus type 2 (PCV2) associated disease among Landrace and Pietrain breeds. The objective of this study was to determine if a difference exists in PCV2 susceptibility between Landrace and Pietrain pigs under experimental conditions. Thirty-nine Landrace pigs and 39 Pietrain pigs were blocked by breed, sire, dam, and litter and randomly divided into the following 4 groups: Landrace noninoculated negative control (Landrace-NEG; n = 13), Pietrain noninoculated negative control (Pietrain-NEG; n = 13), Landrace-PCV2 (n = 26; Landrace), and Pietrain-PCV2 (n = 26; Pietrain). After waning of passively acquired anti-PCV2 antibodies, Landrace-PCV2 and Pietrain-PCV2 groups were inoculated with PCV2 isolate ISU-40895. The Landrace-NEG and Pietrain-NEG groups were housed in a separate room, remained noninoculated, and served as negative controls. All pigs in all groups were necropsied at 21 d post PCV2-inoculation. Onset of seroconversion and ...

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“…At least two of the quantitative trait loci are linked to the interferon-gamma locus and at the interferon receptor locus (Reiner et al, 2002). Genetic differences in the immune response of pigs to antigens or vaccines have been reported for vaccination with pseudorabies vaccine (Rothschild et al, 1984).…”

Section: Discussionmentioning

confidence: 99%

Association analysis between pseudorabies antibody and five single-nucleotide polymorphisms in pigs

Zhang

Jafer

Yuan

et al. 2009

Animal

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Pseudorabies has become endemic and represents a widespread problem for pig production in the world, causing great economic losses associated with reproductive failure and neonatal mortality in the pig industry. Most diseases are the results of mutations of functional genes. Single-nucleotide polymorphisms (SNPs) from the coding regions of the mediators of pro-inflammatory responses or other candidate genes in pigs could indicate their potential involvement in susceptibility or resistance to PrV (pseudorabies virus) infection. There have been no previous association studies with candidate host genes that may influence PrV phenotypic traits. In order to perform association studies to identify genes contributing to PrV phenotypes, the genotypes of five SNPs from four genes ( IL10, CXCL12, BAT2 and EHMT2) were determined for 178 sow samples using a high throughput microarray-based methodology. PrV antibodies were tested by enzyme-linked immunosorbent assay (ELISA) to determine whether there was an association between antibody levels and particular genotypes. The association between SNP genotypes and the PrV antibody levels were analysed using the Duncan method of one-way ANOVA procedure using the SAS (Statistical Analysis Systems) software package. The results showed that the glycoprotein E-ELISA antibody level of pigs with genotypes 11(AA) and 12(AG) was significantly higher than in pigs with genotype 22(GG) ( P , 0.05) of SNP in the gene EHMT2-SNP2. The SNP of EHMT2 may be an effective potential tool to identify susceptible and resistant animals when used in conjunction with traditional selection methods.

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Detection of quantitative trait loci for resistance/susceptibility to pseudorabies virus in swine

Cited by 55 publications

References 23 publications

Evidence of Breed-dependent Differences in Susceptibility to Porcine Circovirus Type-2-associated Disease and Lesions

Evidence of Breed-dependent Differences in Susceptibility to Porcine Circovirus Type-2-associated Disease and Lesions

Difference in severity of porcine circovirus type two-induced pathological lesions between Landrace and Pietrain pigs1

Association analysis between pseudorabies antibody and five single-nucleotide polymorphisms in pigs

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